Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15515 and RR42_RS15520 are separated by 38 nucleotides RR42_RS15520 and RR42_RS15525 are separated by 186 nucleotides RR42_RS15525 and RR42_RS15530 overlap by 4 nucleotides
RR42_RS15515: RR42_RS15515 - hypothetical protein, at 3,349,352 to 3,354,169
_RS15515
RR42_RS15520: RR42_RS15520 - hypothetical protein, at 3,354,208 to 3,354,639
_RS15520
RR42_RS15525: RR42_RS15525 - hypothetical protein, at 3,354,826 to 3,355,266
_RS15525
RR42_RS15530: RR42_RS15530 - membrane protein, at 3,355,263 to 3,356,195
_RS15530
Position (kb)
3354
3355 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3353.237 kb on + strand, within RR42_RS15515 at 3353.237 kb on + strand, within RR42_RS15515 at 3353.238 kb on - strand, within RR42_RS15515 at 3353.510 kb on + strand, within RR42_RS15515 at 3353.564 kb on - strand, within RR42_RS15515 at 3353.672 kb on + strand, within RR42_RS15515 at 3353.673 kb on - strand, within RR42_RS15515 at 3353.684 kb on + strand, within RR42_RS15515 at 3353.685 kb on - strand, within RR42_RS15515 at 3353.685 kb on - strand, within RR42_RS15515 at 3353.714 kb on + strand at 3353.715 kb on - strand at 3353.741 kb on + strand at 3353.742 kb on - strand at 3353.792 kb on + strand at 3353.792 kb on + strand at 3353.818 kb on + strand at 3353.818 kb on + strand at 3353.819 kb on - strand at 3353.819 kb on - strand at 3353.934 kb on + strand at 3353.935 kb on - strand at 3354.028 kb on + strand at 3354.028 kb on + strand at 3354.029 kb on - strand at 3354.180 kb on + strand at 3354.180 kb on + strand at 3354.181 kb on - strand at 3354.181 kb on - strand at 3354.201 kb on + strand at 3354.218 kb on + strand at 3354.219 kb on - strand at 3354.219 kb on - strand at 3354.219 kb on - strand at 3354.249 kb on + strand at 3354.325 kb on + strand, within RR42_RS15520 at 3354.325 kb on + strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.326 kb on - strand, within RR42_RS15520 at 3354.397 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.415 kb on + strand, within RR42_RS15520 at 3354.481 kb on + strand, within RR42_RS15520 at 3354.482 kb on - strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.526 kb on + strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.527 kb on - strand, within RR42_RS15520 at 3354.715 kb on + strand at 3354.716 kb on - strand at 3354.756 kb on - strand at 3354.790 kb on + strand at 3354.998 kb on - strand, within RR42_RS15525 at 3355.378 kb on - strand, within RR42_RS15530 at 3355.458 kb on - strand, within RR42_RS15530 at 3355.556 kb on + strand, within RR42_RS15530 at 3355.557 kb on - strand, within RR42_RS15530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 3,353,237 + RR42_RS15515 0.81 +1.2 3,353,237 + RR42_RS15515 0.81 +0.3 3,353,238 - RR42_RS15515 0.81 -1.3 3,353,510 + RR42_RS15515 0.86 +1.3 3,353,564 - RR42_RS15515 0.87 -0.5 3,353,672 + RR42_RS15515 0.90 -1.4 3,353,673 - RR42_RS15515 0.90 -0.2 3,353,684 + RR42_RS15515 0.90 +1.6 3,353,685 - RR42_RS15515 0.90 +0.1 3,353,685 - RR42_RS15515 0.90 +0.4 3,353,714 + -0.5 3,353,715 - -1.0 3,353,741 + -0.0 3,353,742 - -0.3 3,353,792 + -0.1 3,353,792 + +0.6 3,353,818 + +1.9 3,353,818 + +1.8 3,353,819 - +0.1 3,353,819 - -0.5 3,353,934 + +0.7 3,353,935 - +1.2 3,354,028 + +1.0 3,354,028 + +0.8 3,354,029 - +1.1 3,354,180 + +1.2 3,354,180 + +1.7 3,354,181 - -0.9 3,354,181 - +1.1 3,354,201 + -0.2 3,354,218 + +0.3 3,354,219 - -0.6 3,354,219 - -0.1 3,354,219 - +1.7 3,354,249 + +0.7 3,354,325 + RR42_RS15520 0.27 -0.6 3,354,325 + RR42_RS15520 0.27 -0.6 3,354,326 - RR42_RS15520 0.27 +1.0 3,354,326 - RR42_RS15520 0.27 +1.1 3,354,397 + RR42_RS15520 0.44 -1.9 3,354,415 + RR42_RS15520 0.48 -0.4 3,354,415 + RR42_RS15520 0.48 +1.3 3,354,481 + RR42_RS15520 0.63 -1.7 3,354,482 - RR42_RS15520 0.63 +0.4 3,354,526 + RR42_RS15520 0.74 +0.1 3,354,526 + RR42_RS15520 0.74 -0.7 3,354,527 - RR42_RS15520 0.74 +0.3 3,354,527 - RR42_RS15520 0.74 -0.6 3,354,527 - RR42_RS15520 0.74 +0.4 3,354,715 + +0.3 3,354,716 - -1.1 3,354,756 - -0.5 3,354,790 + -0.7 3,354,998 - RR42_RS15525 0.39 -1.3 3,355,378 - RR42_RS15530 0.12 -1.0 3,355,458 - RR42_RS15530 0.21 +1.0 3,355,556 + RR42_RS15530 0.31 +0.6 3,355,557 - RR42_RS15530 0.32 -1.3
Or see this region's nucleotide sequence