Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14795 and RR42_RS14800 are separated by 150 nucleotides RR42_RS14800 and RR42_RS14805 are separated by 49 nucleotides
RR42_RS14795: RR42_RS14795 - quercetin 2,3-dioxygenase, at 3,187,378 to 3,188,076
_RS14795
RR42_RS14800: RR42_RS14800 - LysR family transcriptional regulator, at 3,188,227 to 3,189,150
_RS14800
RR42_RS14805: RR42_RS14805 - glutamyl-Q tRNA(Asp) ligase, at 3,189,200 to 3,190,141
_RS14805
Position (kb)
3188
3189
3190 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3187.280 kb on + strand at 3187.281 kb on - strand at 3187.284 kb on + strand at 3187.285 kb on - strand at 3187.297 kb on + strand at 3187.297 kb on + strand at 3187.298 kb on - strand at 3187.298 kb on - strand at 3187.302 kb on + strand at 3187.303 kb on - strand at 3187.518 kb on - strand, within RR42_RS14795 at 3187.518 kb on - strand, within RR42_RS14795 at 3187.518 kb on - strand, within RR42_RS14795 at 3187.580 kb on + strand, within RR42_RS14795 at 3187.767 kb on - strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.877 kb on + strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.878 kb on - strand, within RR42_RS14795 at 3187.988 kb on + strand, within RR42_RS14795 at 3187.988 kb on + strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.989 kb on - strand, within RR42_RS14795 at 3187.992 kb on - strand, within RR42_RS14795 at 3188.010 kb on + strand at 3188.010 kb on + strand at 3188.010 kb on + strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.011 kb on - strand at 3188.015 kb on + strand at 3188.042 kb on + strand at 3188.043 kb on - strand at 3188.120 kb on + strand at 3188.121 kb on - strand at 3188.180 kb on - strand at 3188.200 kb on - strand at 3188.332 kb on + strand, within RR42_RS14800 at 3188.332 kb on + strand, within RR42_RS14800 at 3188.332 kb on + strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.333 kb on - strand, within RR42_RS14800 at 3188.572 kb on + strand, within RR42_RS14800 at 3188.573 kb on - strand, within RR42_RS14800 at 3188.573 kb on - strand, within RR42_RS14800 at 3188.678 kb on - strand, within RR42_RS14800 at 3188.678 kb on - strand, within RR42_RS14800 at 3188.829 kb on + strand, within RR42_RS14800 at 3189.149 kb on - strand at 3189.149 kb on - strand at 3189.149 kb on - strand at 3189.206 kb on - strand at 3189.230 kb on + strand at 3189.230 kb on + strand at 3189.468 kb on - strand, within RR42_RS14805 at 3189.510 kb on - strand, within RR42_RS14805 at 3189.510 kb on - strand, within RR42_RS14805 at 3189.765 kb on - strand, within RR42_RS14805 at 3189.893 kb on + strand, within RR42_RS14805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 3,187,280 + -0.2 3,187,281 - -0.3 3,187,284 + -0.8 3,187,285 - +0.5 3,187,297 + -3.4 3,187,297 + +1.1 3,187,298 - -2.2 3,187,298 - -1.3 3,187,302 + -0.6 3,187,303 - -1.9 3,187,518 - RR42_RS14795 0.20 +1.1 3,187,518 - RR42_RS14795 0.20 -0.9 3,187,518 - RR42_RS14795 0.20 +0.1 3,187,580 + RR42_RS14795 0.29 +0.2 3,187,767 - RR42_RS14795 0.56 +0.2 3,187,877 + RR42_RS14795 0.71 -0.1 3,187,877 + RR42_RS14795 0.71 -0.1 3,187,877 + RR42_RS14795 0.71 -0.3 3,187,877 + RR42_RS14795 0.71 -0.6 3,187,877 + RR42_RS14795 0.71 -0.7 3,187,878 - RR42_RS14795 0.72 -1.4 3,187,878 - RR42_RS14795 0.72 -1.1 3,187,878 - RR42_RS14795 0.72 -0.9 3,187,988 + RR42_RS14795 0.87 -0.1 3,187,988 + RR42_RS14795 0.87 -0.4 3,187,989 - RR42_RS14795 0.87 +1.7 3,187,989 - RR42_RS14795 0.87 -0.2 3,187,989 - RR42_RS14795 0.87 -1.3 3,187,989 - RR42_RS14795 0.87 -0.9 3,187,989 - RR42_RS14795 0.87 +0.3 3,187,989 - RR42_RS14795 0.87 +0.1 3,187,989 - RR42_RS14795 0.87 -0.4 3,187,992 - RR42_RS14795 0.88 -0.2 3,188,010 + -0.5 3,188,010 + +0.1 3,188,010 + +0.9 3,188,011 - -0.0 3,188,011 - +0.1 3,188,011 - -0.2 3,188,011 - +0.1 3,188,011 - -1.8 3,188,011 - +0.2 3,188,015 + +1.8 3,188,042 + -1.7 3,188,043 - +0.0 3,188,120 + -2.1 3,188,121 - -0.9 3,188,180 - -1.4 3,188,200 - -2.9 3,188,332 + RR42_RS14800 0.11 -2.1 3,188,332 + RR42_RS14800 0.11 -2.2 3,188,332 + RR42_RS14800 0.11 -0.9 3,188,333 - RR42_RS14800 0.11 -3.0 3,188,333 - RR42_RS14800 0.11 -2.0 3,188,333 - RR42_RS14800 0.11 -1.4 3,188,333 - RR42_RS14800 0.11 -0.2 3,188,333 - RR42_RS14800 0.11 -1.4 3,188,333 - RR42_RS14800 0.11 -1.6 3,188,333 - RR42_RS14800 0.11 -2.3 3,188,333 - RR42_RS14800 0.11 -2.7 3,188,333 - RR42_RS14800 0.11 -2.4 3,188,333 - RR42_RS14800 0.11 -2.0 3,188,333 - RR42_RS14800 0.11 -3.3 3,188,572 + RR42_RS14800 0.37 +0.1 3,188,573 - RR42_RS14800 0.37 +2.1 3,188,573 - RR42_RS14800 0.37 +0.1 3,188,678 - RR42_RS14800 0.49 -1.4 3,188,678 - RR42_RS14800 0.49 -2.2 3,188,829 + RR42_RS14800 0.65 -0.9 3,189,149 - -0.5 3,189,149 - -1.6 3,189,149 - +0.8 3,189,206 - -1.3 3,189,230 + +1.6 3,189,230 + -0.5 3,189,468 - RR42_RS14805 0.28 +0.7 3,189,510 - RR42_RS14805 0.33 -0.9 3,189,510 - RR42_RS14805 0.33 -0.2 3,189,765 - RR42_RS14805 0.60 -0.2 3,189,893 + RR42_RS14805 0.74 -0.0
Or see this region's nucleotide sequence