Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14800

Experiment: R2A with Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14795 and RR42_RS14800 are separated by 150 nucleotidesRR42_RS14800 and RR42_RS14805 are separated by 49 nucleotides RR42_RS14795: RR42_RS14795 - quercetin 2,3-dioxygenase, at 3,187,378 to 3,188,076 _RS14795 RR42_RS14800: RR42_RS14800 - LysR family transcriptional regulator, at 3,188,227 to 3,189,150 _RS14800 RR42_RS14805: RR42_RS14805 - glutamyl-Q tRNA(Asp) ligase, at 3,189,200 to 3,190,141 _RS14805 Position (kb) 3188 3189 3190Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3187.280 kb on + strandat 3187.281 kb on - strandat 3187.284 kb on + strandat 3187.285 kb on - strandat 3187.297 kb on + strandat 3187.297 kb on + strandat 3187.298 kb on - strandat 3187.298 kb on - strandat 3187.302 kb on + strandat 3187.303 kb on - strandat 3187.518 kb on - strand, within RR42_RS14795at 3187.518 kb on - strand, within RR42_RS14795at 3187.518 kb on - strand, within RR42_RS14795at 3187.580 kb on + strand, within RR42_RS14795at 3187.767 kb on - strand, within RR42_RS14795at 3187.877 kb on + strand, within RR42_RS14795at 3187.877 kb on + strand, within RR42_RS14795at 3187.877 kb on + strand, within RR42_RS14795at 3187.877 kb on + strand, within RR42_RS14795at 3187.877 kb on + strand, within RR42_RS14795at 3187.878 kb on - strand, within RR42_RS14795at 3187.878 kb on - strand, within RR42_RS14795at 3187.878 kb on - strand, within RR42_RS14795at 3187.988 kb on + strand, within RR42_RS14795at 3187.988 kb on + strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.989 kb on - strand, within RR42_RS14795at 3187.992 kb on - strand, within RR42_RS14795at 3188.010 kb on + strandat 3188.010 kb on + strandat 3188.010 kb on + strandat 3188.011 kb on - strandat 3188.011 kb on - strandat 3188.011 kb on - strandat 3188.011 kb on - strandat 3188.011 kb on - strandat 3188.011 kb on - strandat 3188.015 kb on + strandat 3188.042 kb on + strandat 3188.043 kb on - strandat 3188.120 kb on + strandat 3188.121 kb on - strandat 3188.180 kb on - strandat 3188.200 kb on - strandat 3188.332 kb on + strand, within RR42_RS14800at 3188.332 kb on + strand, within RR42_RS14800at 3188.332 kb on + strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.333 kb on - strand, within RR42_RS14800at 3188.572 kb on + strand, within RR42_RS14800at 3188.573 kb on - strand, within RR42_RS14800at 3188.573 kb on - strand, within RR42_RS14800at 3188.678 kb on - strand, within RR42_RS14800at 3188.678 kb on - strand, within RR42_RS14800at 3188.829 kb on + strand, within RR42_RS14800at 3189.149 kb on - strandat 3189.149 kb on - strandat 3189.149 kb on - strandat 3189.206 kb on - strandat 3189.230 kb on + strandat 3189.230 kb on + strandat 3189.468 kb on - strand, within RR42_RS14805at 3189.510 kb on - strand, within RR42_RS14805at 3189.510 kb on - strand, within RR42_RS14805at 3189.765 kb on - strand, within RR42_RS14805at 3189.893 kb on + strand, within RR42_RS14805

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM
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3,187,280 + -0.2
3,187,281 - -0.3
3,187,284 + -0.8
3,187,285 - +0.5
3,187,297 + -3.4
3,187,297 + +1.1
3,187,298 - -2.2
3,187,298 - -1.3
3,187,302 + -0.6
3,187,303 - -1.9
3,187,518 - RR42_RS14795 0.20 +1.1
3,187,518 - RR42_RS14795 0.20 -0.9
3,187,518 - RR42_RS14795 0.20 +0.1
3,187,580 + RR42_RS14795 0.29 +0.2
3,187,767 - RR42_RS14795 0.56 +0.2
3,187,877 + RR42_RS14795 0.71 -0.1
3,187,877 + RR42_RS14795 0.71 -0.1
3,187,877 + RR42_RS14795 0.71 -0.3
3,187,877 + RR42_RS14795 0.71 -0.6
3,187,877 + RR42_RS14795 0.71 -0.7
3,187,878 - RR42_RS14795 0.72 -1.4
3,187,878 - RR42_RS14795 0.72 -1.1
3,187,878 - RR42_RS14795 0.72 -0.9
3,187,988 + RR42_RS14795 0.87 -0.1
3,187,988 + RR42_RS14795 0.87 -0.4
3,187,989 - RR42_RS14795 0.87 +1.7
3,187,989 - RR42_RS14795 0.87 -0.2
3,187,989 - RR42_RS14795 0.87 -1.3
3,187,989 - RR42_RS14795 0.87 -0.9
3,187,989 - RR42_RS14795 0.87 +0.3
3,187,989 - RR42_RS14795 0.87 +0.1
3,187,989 - RR42_RS14795 0.87 -0.4
3,187,992 - RR42_RS14795 0.88 -0.2
3,188,010 + -0.5
3,188,010 + +0.1
3,188,010 + +0.9
3,188,011 - -0.0
3,188,011 - +0.1
3,188,011 - -0.2
3,188,011 - +0.1
3,188,011 - -1.8
3,188,011 - +0.2
3,188,015 + +1.8
3,188,042 + -1.7
3,188,043 - +0.0
3,188,120 + -2.1
3,188,121 - -0.9
3,188,180 - -1.4
3,188,200 - -2.9
3,188,332 + RR42_RS14800 0.11 -2.1
3,188,332 + RR42_RS14800 0.11 -2.2
3,188,332 + RR42_RS14800 0.11 -0.9
3,188,333 - RR42_RS14800 0.11 -3.0
3,188,333 - RR42_RS14800 0.11 -2.0
3,188,333 - RR42_RS14800 0.11 -1.4
3,188,333 - RR42_RS14800 0.11 -0.2
3,188,333 - RR42_RS14800 0.11 -1.4
3,188,333 - RR42_RS14800 0.11 -1.6
3,188,333 - RR42_RS14800 0.11 -2.3
3,188,333 - RR42_RS14800 0.11 -2.7
3,188,333 - RR42_RS14800 0.11 -2.4
3,188,333 - RR42_RS14800 0.11 -2.0
3,188,333 - RR42_RS14800 0.11 -3.3
3,188,572 + RR42_RS14800 0.37 +0.1
3,188,573 - RR42_RS14800 0.37 +2.1
3,188,573 - RR42_RS14800 0.37 +0.1
3,188,678 - RR42_RS14800 0.49 -1.4
3,188,678 - RR42_RS14800 0.49 -2.2
3,188,829 + RR42_RS14800 0.65 -0.9
3,189,149 - -0.5
3,189,149 - -1.6
3,189,149 - +0.8
3,189,206 - -1.3
3,189,230 + +1.6
3,189,230 + -0.5
3,189,468 - RR42_RS14805 0.28 +0.7
3,189,510 - RR42_RS14805 0.33 -0.9
3,189,510 - RR42_RS14805 0.33 -0.2
3,189,765 - RR42_RS14805 0.60 -0.2
3,189,893 + RR42_RS14805 0.74 -0.0

Or see this region's nucleotide sequence