Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS14600

Experiment: R2A with Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS14595 and RR42_RS14600 are separated by 156 nucleotidesRR42_RS14600 and RR42_RS14605 are separated by 35 nucleotides RR42_RS14595: RR42_RS14595 - 2-aminoadipate aminotransferase, at 3,139,744 to 3,140,931 _RS14595 RR42_RS14600: RR42_RS14600 - glutathione S-transferase, at 3,141,088 to 3,141,732 _RS14600 RR42_RS14605: RR42_RS14605 - threonine transporter, at 3,141,768 to 3,142,421 _RS14605 Position (kb) 3141 3142Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3140.101 kb on + strand, within RR42_RS14595at 3140.102 kb on - strand, within RR42_RS14595at 3140.225 kb on - strand, within RR42_RS14595at 3140.353 kb on + strand, within RR42_RS14595at 3140.354 kb on - strand, within RR42_RS14595at 3140.407 kb on + strand, within RR42_RS14595at 3140.407 kb on + strand, within RR42_RS14595at 3140.408 kb on - strand, within RR42_RS14595at 3140.408 kb on - strand, within RR42_RS14595at 3140.521 kb on - strand, within RR42_RS14595at 3140.548 kb on + strand, within RR42_RS14595at 3140.548 kb on + strand, within RR42_RS14595at 3140.548 kb on + strand, within RR42_RS14595at 3140.767 kb on + strand, within RR42_RS14595at 3140.767 kb on + strand, within RR42_RS14595at 3140.767 kb on + strand, within RR42_RS14595at 3140.767 kb on + strand, within RR42_RS14595at 3140.768 kb on - strand, within RR42_RS14595at 3140.768 kb on - strand, within RR42_RS14595at 3140.962 kb on + strandat 3140.963 kb on - strandat 3140.963 kb on - strandat 3141.006 kb on + strandat 3141.096 kb on + strandat 3141.217 kb on + strand, within RR42_RS14600at 3141.217 kb on + strand, within RR42_RS14600at 3141.217 kb on + strand, within RR42_RS14600at 3141.217 kb on + strand, within RR42_RS14600at 3141.217 kb on + strand, within RR42_RS14600at 3141.218 kb on - strand, within RR42_RS14600at 3141.218 kb on - strand, within RR42_RS14600at 3141.218 kb on - strand, within RR42_RS14600at 3141.218 kb on - strand, within RR42_RS14600at 3141.296 kb on + strand, within RR42_RS14600at 3141.296 kb on + strand, within RR42_RS14600at 3141.296 kb on + strand, within RR42_RS14600at 3141.297 kb on - strand, within RR42_RS14600at 3141.297 kb on - strand, within RR42_RS14600at 3141.297 kb on - strand, within RR42_RS14600at 3141.326 kb on - strand, within RR42_RS14600at 3141.745 kb on + strandat 3141.745 kb on + strandat 3141.745 kb on + strandat 3141.746 kb on - strandat 3141.746 kb on - strandat 3141.746 kb on - strandat 3141.770 kb on - strandat 3141.907 kb on + strand, within RR42_RS14605at 3141.907 kb on + strand, within RR42_RS14605at 3141.907 kb on + strand, within RR42_RS14605at 3141.907 kb on + strand, within RR42_RS14605at 3141.908 kb on - strand, within RR42_RS14605at 3141.908 kb on - strand, within RR42_RS14605at 3141.908 kb on - strand, within RR42_RS14605at 3142.153 kb on + strand, within RR42_RS14605at 3142.153 kb on + strand, within RR42_RS14605at 3142.154 kb on - strand, within RR42_RS14605at 3142.162 kb on + strand, within RR42_RS14605at 3142.177 kb on + strand, within RR42_RS14605at 3142.178 kb on - strand, within RR42_RS14605at 3142.330 kb on + strand, within RR42_RS14605at 3142.330 kb on + strand, within RR42_RS14605at 3142.330 kb on + strand, within RR42_RS14605at 3142.331 kb on - strand, within RR42_RS14605at 3142.331 kb on - strand, within RR42_RS14605at 3142.331 kb on - strand, within RR42_RS14605at 3142.331 kb on - strand, within RR42_RS14605at 3142.331 kb on - strand, within RR42_RS14605at 3142.476 kb on + strandat 3142.476 kb on + strandat 3142.540 kb on + strandat 3142.540 kb on + strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.541 kb on - strandat 3142.567 kb on + strandat 3142.567 kb on + strandat 3142.568 kb on - strandat 3142.657 kb on + strandat 3142.685 kb on - strandat 3142.728 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM
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3,140,101 + RR42_RS14595 0.30 -1.2
3,140,102 - RR42_RS14595 0.30 -0.3
3,140,225 - RR42_RS14595 0.40 +1.1
3,140,353 + RR42_RS14595 0.51 -0.8
3,140,354 - RR42_RS14595 0.51 +0.3
3,140,407 + RR42_RS14595 0.56 +1.2
3,140,407 + RR42_RS14595 0.56 +0.8
3,140,408 - RR42_RS14595 0.56 +0.0
3,140,408 - RR42_RS14595 0.56 -0.5
3,140,521 - RR42_RS14595 0.65 +0.0
3,140,548 + RR42_RS14595 0.68 -0.6
3,140,548 + RR42_RS14595 0.68 +0.5
3,140,548 + RR42_RS14595 0.68 +3.0
3,140,767 + RR42_RS14595 0.86 +0.3
3,140,767 + RR42_RS14595 0.86 +2.0
3,140,767 + RR42_RS14595 0.86 +2.3
3,140,767 + RR42_RS14595 0.86 -2.2
3,140,768 - RR42_RS14595 0.86 -1.2
3,140,768 - RR42_RS14595 0.86 -0.1
3,140,962 + +0.2
3,140,963 - -2.7
3,140,963 - +0.4
3,141,006 + +0.4
3,141,096 + -0.3
3,141,217 + RR42_RS14600 0.20 -0.7
3,141,217 + RR42_RS14600 0.20 -2.4
3,141,217 + RR42_RS14600 0.20 -1.1
3,141,217 + RR42_RS14600 0.20 +0.5
3,141,217 + RR42_RS14600 0.20 -0.9
3,141,218 - RR42_RS14600 0.20 -1.6
3,141,218 - RR42_RS14600 0.20 -0.8
3,141,218 - RR42_RS14600 0.20 -1.1
3,141,218 - RR42_RS14600 0.20 -0.6
3,141,296 + RR42_RS14600 0.32 -0.5
3,141,296 + RR42_RS14600 0.32 -0.7
3,141,296 + RR42_RS14600 0.32 +1.1
3,141,297 - RR42_RS14600 0.32 -2.7
3,141,297 - RR42_RS14600 0.32 +1.9
3,141,297 - RR42_RS14600 0.32 +0.7
3,141,326 - RR42_RS14600 0.37 -1.3
3,141,745 + +0.4
3,141,745 + -0.7
3,141,745 + +1.4
3,141,746 - +0.7
3,141,746 - -0.1
3,141,746 - -1.0
3,141,770 - -2.8
3,141,907 + RR42_RS14605 0.21 +1.1
3,141,907 + RR42_RS14605 0.21 +0.7
3,141,907 + RR42_RS14605 0.21 -0.8
3,141,907 + RR42_RS14605 0.21 -0.0
3,141,908 - RR42_RS14605 0.21 +0.5
3,141,908 - RR42_RS14605 0.21 +0.0
3,141,908 - RR42_RS14605 0.21 +0.4
3,142,153 + RR42_RS14605 0.59 +1.0
3,142,153 + RR42_RS14605 0.59 +0.1
3,142,154 - RR42_RS14605 0.59 +0.2
3,142,162 + RR42_RS14605 0.60 +0.7
3,142,177 + RR42_RS14605 0.63 +0.0
3,142,178 - RR42_RS14605 0.63 +0.2
3,142,330 + RR42_RS14605 0.86 -0.8
3,142,330 + RR42_RS14605 0.86 +0.2
3,142,330 + RR42_RS14605 0.86 +1.1
3,142,331 - RR42_RS14605 0.86 -1.6
3,142,331 - RR42_RS14605 0.86 -0.6
3,142,331 - RR42_RS14605 0.86 +2.0
3,142,331 - RR42_RS14605 0.86 -0.3
3,142,331 - RR42_RS14605 0.86 +1.0
3,142,476 + +1.0
3,142,476 + +0.3
3,142,540 + -1.0
3,142,540 + -0.7
3,142,541 - -1.4
3,142,541 - -0.4
3,142,541 - -0.6
3,142,541 - -0.4
3,142,541 - +0.0
3,142,541 - -0.1
3,142,541 - -0.2
3,142,567 + -0.6
3,142,567 + -0.2
3,142,568 - +0.4
3,142,657 + -0.2
3,142,685 - -0.2
3,142,728 - +0.6

Or see this region's nucleotide sequence