Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS11120

Experiment: R2A with Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS11110 and RR42_RS11115 are separated by 28 nucleotidesRR42_RS11115 and RR42_RS11120 overlap by 11 nucleotidesRR42_RS11120 and RR42_RS11125 are separated by 230 nucleotides RR42_RS11110: RR42_RS11110 - serine dehydratase, at 2,373,021 to 2,373,983 _RS11110 RR42_RS11115: RR42_RS11115 - ornithine cyclodeaminase, at 2,374,012 to 2,374,983 _RS11115 RR42_RS11120: RR42_RS11120 - DNA-binding protein, at 2,374,973 to 2,375,614 _RS11120 RR42_RS11125: RR42_RS11125 - glycerophosphodiester phosphodiesterase, at 2,375,845 to 2,377,218 _RS11125 Position (kb) 2374 2375 2376Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2374.387 kb on + strand, within RR42_RS11115at 2374.388 kb on - strand, within RR42_RS11115at 2374.388 kb on - strand, within RR42_RS11115at 2374.388 kb on - strand, within RR42_RS11115at 2374.497 kb on - strand, within RR42_RS11115at 2374.497 kb on - strand, within RR42_RS11115at 2374.879 kb on - strand, within RR42_RS11115at 2374.879 kb on - strand, within RR42_RS11115at 2374.983 kb on + strandat 2374.984 kb on - strandat 2374.987 kb on + strandat 2374.987 kb on + strandat 2374.988 kb on - strandat 2374.988 kb on - strandat 2374.988 kb on - strandat 2374.988 kb on - strandat 2374.988 kb on - strandat 2374.989 kb on + strandat 2374.989 kb on + strandat 2374.990 kb on - strandat 2375.293 kb on - strand, within RR42_RS11120at 2375.364 kb on + strand, within RR42_RS11120at 2375.365 kb on - strand, within RR42_RS11120at 2375.386 kb on - strand, within RR42_RS11120at 2375.603 kb on - strandat 2375.603 kb on - strandat 2375.704 kb on + strandat 2375.705 kb on - strandat 2375.705 kb on - strandat 2375.705 kb on - strandat 2375.843 kb on - strandat 2375.843 kb on - strandat 2375.989 kb on + strand, within RR42_RS11125at 2375.990 kb on - strand, within RR42_RS11125at 2375.990 kb on - strand, within RR42_RS11125at 2375.992 kb on - strand, within RR42_RS11125at 2375.992 kb on - strand, within RR42_RS11125at 2376.250 kb on + strand, within RR42_RS11125at 2376.250 kb on + strand, within RR42_RS11125at 2376.251 kb on - strand, within RR42_RS11125at 2376.251 kb on - strand, within RR42_RS11125at 2376.253 kb on + strand, within RR42_RS11125at 2376.361 kb on + strand, within RR42_RS11125at 2376.362 kb on - strand, within RR42_RS11125at 2376.362 kb on - strand, within RR42_RS11125

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM
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2,374,387 + RR42_RS11115 0.39 -1.0
2,374,388 - RR42_RS11115 0.39 -0.2
2,374,388 - RR42_RS11115 0.39 -0.8
2,374,388 - RR42_RS11115 0.39 -0.0
2,374,497 - RR42_RS11115 0.50 +0.1
2,374,497 - RR42_RS11115 0.50 -0.8
2,374,879 - RR42_RS11115 0.89 +0.2
2,374,879 - RR42_RS11115 0.89 -0.4
2,374,983 + -0.2
2,374,984 - -0.1
2,374,987 + +0.2
2,374,987 + +0.3
2,374,988 - -0.5
2,374,988 - -1.6
2,374,988 - +0.2
2,374,988 - -2.9
2,374,988 - +0.3
2,374,989 + -0.8
2,374,989 + -2.1
2,374,990 - -0.7
2,375,293 - RR42_RS11120 0.50 -1.9
2,375,364 + RR42_RS11120 0.61 -3.1
2,375,365 - RR42_RS11120 0.61 -1.0
2,375,386 - RR42_RS11120 0.64 -2.4
2,375,603 - -4.8
2,375,603 - -3.0
2,375,704 + -1.0
2,375,705 - -1.1
2,375,705 - +0.5
2,375,705 - -0.7
2,375,843 - +0.8
2,375,843 - -0.7
2,375,989 + RR42_RS11125 0.10 +1.8
2,375,990 - RR42_RS11125 0.11 -0.9
2,375,990 - RR42_RS11125 0.11 +0.1
2,375,992 - RR42_RS11125 0.11 -0.2
2,375,992 - RR42_RS11125 0.11 -2.5
2,376,250 + RR42_RS11125 0.29 -0.4
2,376,250 + RR42_RS11125 0.29 +1.8
2,376,251 - RR42_RS11125 0.30 -1.2
2,376,251 - RR42_RS11125 0.30 +1.2
2,376,253 + RR42_RS11125 0.30 +0.5
2,376,361 + RR42_RS11125 0.38 -0.9
2,376,362 - RR42_RS11125 0.38 +0.5
2,376,362 - RR42_RS11125 0.38 +0.4

Or see this region's nucleotide sequence