Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10995 and RR42_RS11000 are separated by 116 nucleotides RR42_RS11000 and RR42_RS11005 are separated by 316 nucleotides
RR42_RS10995: RR42_RS10995 - hypothetical protein, at 2,347,942 to 2,348,928
_RS10995
RR42_RS11000: RR42_RS11000 - alpha/beta hydrolase, at 2,349,045 to 2,350,010
_RS11000
RR42_RS11005: RR42_RS11005 - 4-hydroxybutyrate dehydrogenase, at 2,350,327 to 2,351,475
_RS11005
Position (kb)
2349
2350
2351 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2348.075 kb on + strand, within RR42_RS10995 at 2348.075 kb on + strand, within RR42_RS10995 at 2348.279 kb on + strand, within RR42_RS10995 at 2348.279 kb on + strand, within RR42_RS10995 at 2348.280 kb on - strand, within RR42_RS10995 at 2348.360 kb on + strand, within RR42_RS10995 at 2348.447 kb on + strand, within RR42_RS10995 at 2348.447 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.561 kb on + strand, within RR42_RS10995 at 2348.562 kb on - strand, within RR42_RS10995 at 2348.604 kb on - strand, within RR42_RS10995 at 2348.646 kb on - strand, within RR42_RS10995 at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.956 kb on - strand at 2348.956 kb on - strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.962 kb on - strand at 2348.962 kb on - strand at 2348.969 kb on + strand at 2348.975 kb on + strand at 2348.976 kb on - strand at 2349.151 kb on + strand, within RR42_RS11000 at 2349.152 kb on - strand, within RR42_RS11000 at 2349.152 kb on - strand, within RR42_RS11000 at 2349.230 kb on - strand, within RR42_RS11000 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.382 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.646 kb on + strand, within RR42_RS11000 at 2349.749 kb on - strand, within RR42_RS11000 at 2349.796 kb on + strand, within RR42_RS11000 at 2349.797 kb on - strand, within RR42_RS11000 at 2350.175 kb on + strand at 2350.176 kb on - strand at 2350.206 kb on - strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.324 kb on + strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.325 kb on - strand at 2350.509 kb on - strand, within RR42_RS11005 at 2350.672 kb on + strand, within RR42_RS11005 at 2350.672 kb on + strand, within RR42_RS11005 at 2350.673 kb on - strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2350.844 kb on + strand, within RR42_RS11005 at 2351.001 kb on + strand, within RR42_RS11005 at 2351.002 kb on - strand, within RR42_RS11005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,348,075 + RR42_RS10995 0.13 +1.8 2,348,075 + RR42_RS10995 0.13 +1.1 2,348,279 + RR42_RS10995 0.34 -1.2 2,348,279 + RR42_RS10995 0.34 +0.3 2,348,280 - RR42_RS10995 0.34 -0.6 2,348,360 + RR42_RS10995 0.42 -0.2 2,348,447 + RR42_RS10995 0.51 -0.9 2,348,447 + RR42_RS10995 0.51 -0.8 2,348,486 + RR42_RS10995 0.55 +0.4 2,348,486 + RR42_RS10995 0.55 +0.9 2,348,486 + RR42_RS10995 0.55 -0.7 2,348,487 - RR42_RS10995 0.55 -0.2 2,348,487 - RR42_RS10995 0.55 +0.9 2,348,487 - RR42_RS10995 0.55 -1.9 2,348,487 - RR42_RS10995 0.55 -1.2 2,348,487 - RR42_RS10995 0.55 -0.6 2,348,487 - RR42_RS10995 0.55 -0.6 2,348,487 - RR42_RS10995 0.55 +0.6 2,348,487 - RR42_RS10995 0.55 -0.7 2,348,561 + RR42_RS10995 0.63 -1.3 2,348,562 - RR42_RS10995 0.63 -0.6 2,348,604 - RR42_RS10995 0.67 +0.6 2,348,646 - RR42_RS10995 0.71 -0.8 2,348,955 + -0.7 2,348,955 + -1.8 2,348,955 + +1.3 2,348,955 + -0.7 2,348,955 + -1.1 2,348,955 + -2.3 2,348,956 - -0.5 2,348,956 - +1.2 2,348,961 + -0.2 2,348,961 + -0.6 2,348,961 + -0.8 2,348,961 + -0.1 2,348,961 + -0.1 2,348,961 + -0.3 2,348,961 + +0.2 2,348,962 - -0.2 2,348,962 - -2.1 2,348,969 + +1.8 2,348,975 + +1.2 2,348,976 - +0.5 2,349,151 + RR42_RS11000 0.11 +0.5 2,349,152 - RR42_RS11000 0.11 +1.2 2,349,152 - RR42_RS11000 0.11 +1.4 2,349,230 - RR42_RS11000 0.19 -0.9 2,349,341 - RR42_RS11000 0.31 +0.9 2,349,341 - RR42_RS11000 0.31 -0.2 2,349,382 + RR42_RS11000 0.35 -0.7 2,349,439 + RR42_RS11000 0.41 -0.9 2,349,439 + RR42_RS11000 0.41 +0.2 2,349,439 + RR42_RS11000 0.41 -0.1 2,349,440 - RR42_RS11000 0.41 -1.7 2,349,440 - RR42_RS11000 0.41 +1.8 2,349,440 - RR42_RS11000 0.41 -1.1 2,349,440 - RR42_RS11000 0.41 -1.5 2,349,646 + RR42_RS11000 0.62 -1.6 2,349,749 - RR42_RS11000 0.73 -0.7 2,349,796 + RR42_RS11000 0.78 -1.2 2,349,797 - RR42_RS11000 0.78 +1.3 2,350,175 + +0.2 2,350,176 - +0.1 2,350,206 - -2.9 2,350,324 + +0.0 2,350,324 + -0.6 2,350,324 + -1.8 2,350,324 + -0.8 2,350,324 + +0.4 2,350,324 + +0.5 2,350,325 - -0.4 2,350,325 - -0.1 2,350,325 - -0.8 2,350,325 - -0.2 2,350,325 - -0.6 2,350,325 - +0.4 2,350,325 - +0.2 2,350,509 - RR42_RS11005 0.16 -0.2 2,350,672 + RR42_RS11005 0.30 +0.8 2,350,672 + RR42_RS11005 0.30 +1.1 2,350,673 - RR42_RS11005 0.30 +2.3 2,350,844 + RR42_RS11005 0.45 -0.2 2,350,844 + RR42_RS11005 0.45 -0.2 2,350,844 + RR42_RS11005 0.45 +1.5 2,350,844 + RR42_RS11005 0.45 -1.8 2,351,001 + RR42_RS11005 0.59 -0.8 2,351,002 - RR42_RS11005 0.59 -0.3
Or see this region's nucleotide sequence