Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09425 and RR42_RS09430 are separated by 102 nucleotides RR42_RS09430 and RR42_RS09435 are separated by 272 nucleotides RR42_RS09435 and RR42_RS09440 are separated by 91 nucleotides
RR42_RS09425: RR42_RS09425 - carotenoid oxygenase, at 1,999,526 to 2,000,914
_RS09425
RR42_RS09430: RR42_RS09430 - hypothetical protein, at 2,001,017 to 2,002,525
_RS09430
RR42_RS09435: RR42_RS09435 - DNA glycosylase, at 2,002,798 to 2,003,307
_RS09435
RR42_RS09440: RR42_RS09440 - C4-dicarboxylate ABC transporter, at 2,003,399 to 2,004,388
_RS09440
Position (kb)
2001
2002
2003 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2000.046 kb on + strand, within RR42_RS09425 at 2000.046 kb on + strand, within RR42_RS09425 at 2000.047 kb on - strand, within RR42_RS09425 at 2000.047 kb on - strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.112 kb on + strand, within RR42_RS09425 at 2000.113 kb on - strand, within RR42_RS09425 at 2000.323 kb on + strand, within RR42_RS09425 at 2000.324 kb on - strand, within RR42_RS09425 at 2000.533 kb on + strand, within RR42_RS09425 at 2000.534 kb on - strand, within RR42_RS09425 at 2000.779 kb on - strand at 2000.870 kb on - strand at 2000.917 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2000.971 kb on - strand at 2001.132 kb on + strand at 2001.133 kb on - strand at 2001.309 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.313 kb on + strand, within RR42_RS09430 at 2001.314 kb on - strand, within RR42_RS09430 at 2001.314 kb on - strand, within RR42_RS09430 at 2001.384 kb on + strand, within RR42_RS09430 at 2001.453 kb on + strand, within RR42_RS09430 at 2001.486 kb on + strand, within RR42_RS09430 at 2001.487 kb on - strand, within RR42_RS09430 at 2001.618 kb on + strand, within RR42_RS09430 at 2001.619 kb on - strand, within RR42_RS09430 at 2001.640 kb on - strand, within RR42_RS09430 at 2001.640 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.787 kb on - strand, within RR42_RS09430 at 2001.804 kb on + strand, within RR42_RS09430 at 2001.804 kb on + strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.805 kb on - strand, within RR42_RS09430 at 2001.909 kb on + strand, within RR42_RS09430 at 2001.944 kb on + strand, within RR42_RS09430 at 2001.944 kb on + strand, within RR42_RS09430 at 2001.944 kb on + strand, within RR42_RS09430 at 2001.945 kb on - strand, within RR42_RS09430 at 2001.945 kb on - strand, within RR42_RS09430 at 2001.948 kb on + strand, within RR42_RS09430 at 2001.948 kb on + strand, within RR42_RS09430 at 2001.949 kb on - strand, within RR42_RS09430 at 2001.949 kb on - strand, within RR42_RS09430 at 2001.975 kb on + strand, within RR42_RS09430 at 2002.081 kb on + strand, within RR42_RS09430 at 2002.082 kb on - strand, within RR42_RS09430 at 2002.082 kb on - strand, within RR42_RS09430 at 2002.083 kb on + strand, within RR42_RS09430 at 2002.294 kb on + strand, within RR42_RS09430 at 2002.295 kb on - strand, within RR42_RS09430 at 2002.295 kb on - strand, within RR42_RS09430 at 2002.342 kb on - strand, within RR42_RS09430 at 2002.342 kb on - strand, within RR42_RS09430 at 2002.380 kb on + strand at 2002.381 kb on - strand at 2002.381 kb on - strand at 2002.381 kb on - strand at 2002.491 kb on + strand at 2002.491 kb on + strand at 2002.511 kb on + strand at 2002.585 kb on + strand at 2002.609 kb on + strand at 2002.610 kb on - strand at 2002.610 kb on - strand at 2002.610 kb on - strand at 2002.642 kb on + strand at 2002.829 kb on + strand at 2002.830 kb on - strand at 2002.830 kb on - strand at 2002.838 kb on + strand at 2002.839 kb on - strand at 2002.849 kb on - strand, within RR42_RS09435 at 2002.986 kb on - strand, within RR42_RS09435 at 2003.010 kb on + strand, within RR42_RS09435 at 2003.044 kb on + strand, within RR42_RS09435 at 2003.100 kb on + strand, within RR42_RS09435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 2,000,046 + RR42_RS09425 0.37 -0.6 2,000,046 + RR42_RS09425 0.37 -0.5 2,000,047 - RR42_RS09425 0.38 -0.1 2,000,047 - RR42_RS09425 0.38 -0.3 2,000,112 + RR42_RS09425 0.42 -0.6 2,000,112 + RR42_RS09425 0.42 +0.5 2,000,112 + RR42_RS09425 0.42 -0.0 2,000,112 + RR42_RS09425 0.42 -0.2 2,000,112 + RR42_RS09425 0.42 -0.3 2,000,113 - RR42_RS09425 0.42 -1.5 2,000,323 + RR42_RS09425 0.57 -1.7 2,000,324 - RR42_RS09425 0.57 -0.4 2,000,533 + RR42_RS09425 0.72 -0.3 2,000,534 - RR42_RS09425 0.73 -0.8 2,000,779 - -0.2 2,000,870 - +0.2 2,000,917 - +0.3 2,000,971 - +0.2 2,000,971 - +0.3 2,000,971 - -2.2 2,000,971 - +0.5 2,000,971 - -1.9 2,001,132 + +0.7 2,001,133 - -0.3 2,001,309 + RR42_RS09430 0.19 +2.7 2,001,313 + RR42_RS09430 0.20 +0.0 2,001,313 + RR42_RS09430 0.20 -1.0 2,001,313 + RR42_RS09430 0.20 -0.6 2,001,314 - RR42_RS09430 0.20 +0.3 2,001,314 - RR42_RS09430 0.20 -0.9 2,001,384 + RR42_RS09430 0.24 +0.7 2,001,453 + RR42_RS09430 0.29 -0.8 2,001,486 + RR42_RS09430 0.31 +0.1 2,001,487 - RR42_RS09430 0.31 -0.2 2,001,618 + RR42_RS09430 0.40 -0.3 2,001,619 - RR42_RS09430 0.40 -0.1 2,001,640 - RR42_RS09430 0.41 +2.1 2,001,640 - RR42_RS09430 0.41 -0.7 2,001,787 - RR42_RS09430 0.51 +0.4 2,001,787 - RR42_RS09430 0.51 +0.3 2,001,787 - RR42_RS09430 0.51 -2.7 2,001,804 + RR42_RS09430 0.52 +0.4 2,001,804 + RR42_RS09430 0.52 +0.0 2,001,805 - RR42_RS09430 0.52 -0.4 2,001,805 - RR42_RS09430 0.52 -0.5 2,001,805 - RR42_RS09430 0.52 -0.5 2,001,909 + RR42_RS09430 0.59 -1.0 2,001,944 + RR42_RS09430 0.61 -0.8 2,001,944 + RR42_RS09430 0.61 -1.1 2,001,944 + RR42_RS09430 0.61 -0.1 2,001,945 - RR42_RS09430 0.61 -0.8 2,001,945 - RR42_RS09430 0.61 -2.5 2,001,948 + RR42_RS09430 0.62 -0.6 2,001,948 + RR42_RS09430 0.62 +1.5 2,001,949 - RR42_RS09430 0.62 -0.1 2,001,949 - RR42_RS09430 0.62 -0.3 2,001,975 + RR42_RS09430 0.63 +0.5 2,002,081 + RR42_RS09430 0.71 -0.9 2,002,082 - RR42_RS09430 0.71 -0.2 2,002,082 - RR42_RS09430 0.71 +0.1 2,002,083 + RR42_RS09430 0.71 +0.3 2,002,294 + RR42_RS09430 0.85 -0.5 2,002,295 - RR42_RS09430 0.85 +0.2 2,002,295 - RR42_RS09430 0.85 -1.6 2,002,342 - RR42_RS09430 0.88 +1.7 2,002,342 - RR42_RS09430 0.88 -0.3 2,002,380 + +0.9 2,002,381 - +0.5 2,002,381 - +1.5 2,002,381 - +0.2 2,002,491 + -1.9 2,002,491 + -2.0 2,002,511 + -1.1 2,002,585 + -0.5 2,002,609 + -0.9 2,002,610 - +0.7 2,002,610 - -0.1 2,002,610 - +0.7 2,002,642 + +1.1 2,002,829 + -0.2 2,002,830 - +0.8 2,002,830 - +0.4 2,002,838 + +1.1 2,002,839 - +1.4 2,002,849 - RR42_RS09435 0.10 +0.1 2,002,986 - RR42_RS09435 0.37 +0.7 2,003,010 + RR42_RS09435 0.42 +0.2 2,003,044 + RR42_RS09435 0.48 +0.1 2,003,100 + RR42_RS09435 0.59 +0.5
Or see this region's nucleotide sequence