Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04960 and RR42_RS04965 are separated by 705 nucleotides RR42_RS04965 and RR42_RS04970 are separated by 209 nucleotides
RR42_RS04960: RR42_RS04960 - Fe(II)-dependent oxygenase, at 1,096,205 to 1,096,888
_RS04960
RR42_RS04965: RR42_RS04965 - hypothetical protein, at 1,097,594 to 1,097,905
_RS04965
RR42_RS04970: RR42_RS04970 - aspartyl beta-hydroxylase, at 1,098,115 to 1,099,017
_RS04970
Position (kb)
1097
1098 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1096.612 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.617 kb on - strand, within RR42_RS04960 at 1096.617 kb on - strand, within RR42_RS04960 at 1096.651 kb on + strand, within RR42_RS04960 at 1096.651 kb on + strand, within RR42_RS04960 at 1096.662 kb on - strand, within RR42_RS04960 at 1096.854 kb on - strand at 1097.074 kb on + strand at 1097.123 kb on + strand at 1097.123 kb on + strand at 1097.156 kb on + strand at 1097.156 kb on + strand at 1097.157 kb on - strand at 1097.157 kb on - strand at 1097.269 kb on + strand at 1097.270 kb on - strand at 1097.300 kb on + strand at 1097.301 kb on - strand at 1097.301 kb on - strand at 1097.301 kb on - strand at 1097.355 kb on + strand at 1097.356 kb on - strand at 1097.356 kb on - strand at 1097.359 kb on - strand at 1097.378 kb on - strand at 1097.378 kb on - strand at 1097.414 kb on + strand at 1097.421 kb on + strand at 1097.447 kb on + strand at 1097.541 kb on + strand at 1097.541 kb on + strand at 1097.655 kb on - strand, within RR42_RS04965 at 1097.655 kb on - strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.807 kb on - strand, within RR42_RS04965 at 1097.929 kb on + strand at 1097.929 kb on + strand at 1097.930 kb on - strand at 1097.939 kb on - strand at 1097.947 kb on + strand at 1098.031 kb on - strand at 1098.035 kb on + strand at 1098.069 kb on - strand at 1098.069 kb on - strand at 1098.074 kb on + strand at 1098.074 kb on + strand at 1098.100 kb on + strand at 1098.100 kb on + strand at 1098.160 kb on + strand at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.254 kb on + strand, within RR42_RS04970 at 1098.255 kb on - strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.428 kb on - strand, within RR42_RS04970 at 1098.445 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on - strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.716 kb on - strand, within RR42_RS04970 at 1098.901 kb on - strand, within RR42_RS04970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 1,096,612 + RR42_RS04960 0.60 +0.0 1,096,616 + RR42_RS04960 0.60 -0.3 1,096,616 + RR42_RS04960 0.60 -2.0 1,096,616 + RR42_RS04960 0.60 +1.0 1,096,617 - RR42_RS04960 0.60 -1.2 1,096,617 - RR42_RS04960 0.60 -1.2 1,096,651 + RR42_RS04960 0.65 -0.3 1,096,651 + RR42_RS04960 0.65 -2.7 1,096,662 - RR42_RS04960 0.67 +0.0 1,096,854 - -1.0 1,097,074 + +0.0 1,097,123 + -1.8 1,097,123 + +0.3 1,097,156 + -0.6 1,097,156 + -1.3 1,097,157 - -2.1 1,097,157 - -0.1 1,097,269 + -1.8 1,097,270 - -1.5 1,097,300 + -0.3 1,097,301 - -2.9 1,097,301 - -1.4 1,097,301 - +0.8 1,097,355 + -1.2 1,097,356 - -0.5 1,097,356 - +0.4 1,097,359 - +0.3 1,097,378 - +0.3 1,097,378 - +0.6 1,097,414 + -1.3 1,097,421 + -1.3 1,097,447 + -1.2 1,097,541 + -0.3 1,097,541 + +0.4 1,097,655 - RR42_RS04965 0.20 +0.0 1,097,655 - RR42_RS04965 0.20 -0.3 1,097,806 + RR42_RS04965 0.68 -0.4 1,097,806 + RR42_RS04965 0.68 +1.7 1,097,806 + RR42_RS04965 0.68 +1.4 1,097,807 - RR42_RS04965 0.68 -0.7 1,097,929 + -2.2 1,097,929 + -0.1 1,097,930 - +0.3 1,097,939 - -0.2 1,097,947 + +0.4 1,098,031 - +0.8 1,098,035 + -0.3 1,098,069 - -1.6 1,098,069 - -1.0 1,098,074 + -0.2 1,098,074 + -1.1 1,098,100 + -1.0 1,098,100 + +0.1 1,098,160 + -0.7 1,098,250 + RR42_RS04970 0.15 +0.3 1,098,250 + RR42_RS04970 0.15 -0.3 1,098,250 + RR42_RS04970 0.15 -1.9 1,098,250 + RR42_RS04970 0.15 -0.2 1,098,250 + RR42_RS04970 0.15 -1.2 1,098,251 - RR42_RS04970 0.15 -0.1 1,098,251 - RR42_RS04970 0.15 +1.0 1,098,251 - RR42_RS04970 0.15 -0.9 1,098,251 - RR42_RS04970 0.15 -1.0 1,098,251 - RR42_RS04970 0.15 -2.0 1,098,254 + RR42_RS04970 0.15 -1.2 1,098,255 - RR42_RS04970 0.16 +0.7 1,098,280 + RR42_RS04970 0.18 -0.9 1,098,280 + RR42_RS04970 0.18 -1.0 1,098,280 + RR42_RS04970 0.18 -0.7 1,098,281 - RR42_RS04970 0.18 +0.4 1,098,281 - RR42_RS04970 0.18 +1.3 1,098,281 - RR42_RS04970 0.18 -0.8 1,098,281 - RR42_RS04970 0.18 -0.5 1,098,428 - RR42_RS04970 0.35 -0.4 1,098,445 + RR42_RS04970 0.37 +0.2 1,098,675 + RR42_RS04970 0.62 +1.0 1,098,675 + RR42_RS04970 0.62 -0.2 1,098,675 + RR42_RS04970 0.62 +0.4 1,098,676 - RR42_RS04970 0.62 -0.2 1,098,676 - RR42_RS04970 0.62 -1.0 1,098,676 - RR42_RS04970 0.62 -0.3 1,098,701 + RR42_RS04970 0.65 -0.4 1,098,701 + RR42_RS04970 0.65 -0.1 1,098,701 - RR42_RS04970 0.65 -0.4 1,098,715 + RR42_RS04970 0.66 -1.4 1,098,715 + RR42_RS04970 0.66 +0.6 1,098,716 - RR42_RS04970 0.67 -1.2 1,098,901 - RR42_RS04970 0.87 +0.2
Or see this region's nucleotide sequence