Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02830 and RR42_RS02835 are separated by 480 nucleotides RR42_RS02835 and RR42_RS02840 overlap by 4 nucleotides
RR42_RS02830: RR42_RS02830 - glycyl-tRNA synthetase subunit alpha, at 629,777 to 630,727
_RS02830
RR42_RS02835: RR42_RS02835 - acyltransferase, at 631,208 to 632,815
_RS02835
RR42_RS02840: RR42_RS02840 - cation transporter, at 632,812 to 633,684
_RS02840
Position (kb) 631
632
633 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 630.739 kb on - strand at 630.742 kb on - strand at 630.742 kb on - strand at 630.879 kb on + strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.880 kb on - strand at 630.963 kb on + strand at 630.963 kb on + strand at 630.999 kb on + strand at 631.007 kb on - strand at 631.010 kb on - strand at 631.096 kb on + strand at 631.097 kb on - strand at 631.204 kb on - strand at 632.816 kb on - strand at 633.183 kb on - strand, within RR42_RS02840 at 633.241 kb on - strand, within RR42_RS02840 at 633.315 kb on - strand, within RR42_RS02840 at 633.315 kb on - strand, within RR42_RS02840 at 633.318 kb on - strand, within RR42_RS02840 at 633.375 kb on - strand, within RR42_RS02840 at 633.500 kb on - strand, within RR42_RS02840 at 633.726 kb on + strand at 633.726 kb on + strand at 633.726 kb on + strand at 633.727 kb on - strand at 633.727 kb on - strand at 633.727 kb on - strand at 633.727 kb on - strand at 633.727 kb on - strand at 633.799 kb on + strand at 633.799 kb on + strand at 633.800 kb on - strand at 633.800 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 630,739 - +0.6 630,742 - -0.6 630,742 - -1.2 630,879 + -1.4 630,880 - -2.9 630,880 - -3.1 630,880 - -2.3 630,880 - +1.4 630,880 - +2.2 630,880 - -0.6 630,880 - -0.5 630,880 - -1.3 630,963 + -1.3 630,963 + -0.3 630,999 + -0.2 631,007 - -0.1 631,010 - -3.0 631,096 + +1.2 631,097 - -0.2 631,204 - -0.8 632,816 - -1.4 633,183 - RR42_RS02840 0.42 +1.7 633,241 - RR42_RS02840 0.49 +2.9 633,315 - RR42_RS02840 0.58 +1.7 633,315 - RR42_RS02840 0.58 +1.9 633,318 - RR42_RS02840 0.58 +1.6 633,375 - RR42_RS02840 0.64 +1.2 633,500 - RR42_RS02840 0.79 +0.2 633,726 + +4.5 633,726 + +0.2 633,726 + +1.7 633,727 - +0.2 633,727 - -0.5 633,727 - +2.2 633,727 - +1.3 633,727 - +0.8 633,799 + +0.2 633,799 + +1.2 633,800 - -0.2 633,800 - +0.1
Or see this region's nucleotide sequence