Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02200 and RR42_RS02205 are separated by 65 nucleotides RR42_RS02205 and RR42_RS02210 overlap by 4 nucleotides RR42_RS02210 and RR42_RS02215 overlap by 4 nucleotides RR42_RS02215 and RR42_RS02220 are separated by 202 nucleotides
RR42_RS02200: RR42_RS02200 - LacI family transcriptional regulator, at 489,135 to 490,130
_RS02200
RR42_RS02205: RR42_RS02205 - succinate dehydrogenase, at 490,196 to 490,549
_RS02205
RR42_RS02210: RR42_RS02210 - succinate dehydrogenase, at 490,546 to 490,899
_RS02210
RR42_RS02215: RR42_RS02215 - fumarate reductase, at 490,896 to 491,609
_RS02215
RR42_RS02220: RR42_RS02220 - LysR family transcriptional regulator, at 491,812 to 492,753
_RS02220
Position (kb) 490
491 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 489.560 kb on - strand, within RR42_RS02200 at 489.560 kb on - strand, within RR42_RS02200 at 489.759 kb on - strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 490.197 kb on + strand at 490.197 kb on + strand at 490.510 kb on - strand, within RR42_RS02205 at 490.566 kb on - strand at 490.856 kb on + strand, within RR42_RS02210 at 490.857 kb on - strand, within RR42_RS02210 at 490.900 kb on - strand at 490.900 kb on - strand at 490.900 kb on - strand at 491.003 kb on + strand, within RR42_RS02215 at 491.004 kb on - strand, within RR42_RS02215 at 491.503 kb on + strand, within RR42_RS02215 at 491.504 kb on - strand, within RR42_RS02215 at 491.551 kb on + strand at 491.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 489,560 - RR42_RS02200 0.43 -0.2 489,560 - RR42_RS02200 0.43 -0.6 489,759 - RR42_RS02200 0.63 -0.2 489,845 + RR42_RS02200 0.71 +1.6 489,845 + RR42_RS02200 0.71 +0.2 489,845 + RR42_RS02200 0.71 +0.9 489,846 - RR42_RS02200 0.71 -0.3 489,846 - RR42_RS02200 0.71 +1.8 489,848 - RR42_RS02200 0.72 +1.4 489,848 - RR42_RS02200 0.72 +0.6 490,197 + +0.7 490,197 + +1.3 490,510 - RR42_RS02205 0.89 -1.3 490,566 - -1.8 490,856 + RR42_RS02210 0.88 -1.3 490,857 - RR42_RS02210 0.88 +3.2 490,900 - +0.2 490,900 - +0.2 490,900 - +0.4 491,003 + RR42_RS02215 0.15 +0.2 491,004 - RR42_RS02215 0.15 +0.9 491,503 + RR42_RS02215 0.85 +0.3 491,504 - RR42_RS02215 0.85 -0.5 491,551 + -0.1 491,587 - +1.1
Or see this region's nucleotide sequence