Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02110 and RR42_RS02115 are separated by 38 nucleotides RR42_RS02115 and RR42_RS02120 are separated by 68 nucleotides RR42_RS02120 and RR42_RS02125 are separated by 161 nucleotides
RR42_RS02110: RR42_RS02110 - potassium transporter, at 468,117 to 470,096
_RS02110
RR42_RS02115: RR42_RS02115 - arylsulfate sulfotransferase, at 470,135 to 470,518
_RS02115
RR42_RS02120: RR42_RS02120 - glutamine amidotransferase, at 470,587 to 471,165
_RS02120
RR42_RS02125: RR42_RS02125 - adenine phosphoribosyltransferase, at 471,327 to 471,899
_RS02125
Position (kb)
470
471
472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 469.661 kb on + strand, within RR42_RS02110 at 469.803 kb on + strand, within RR42_RS02110 at 469.822 kb on - strand, within RR42_RS02110 at 469.822 kb on - strand, within RR42_RS02110 at 469.822 kb on - strand, within RR42_RS02110 at 470.105 kb on + strand at 470.105 kb on + strand at 470.105 kb on + strand at 470.105 kb on + strand at 470.106 kb on - strand at 470.275 kb on - strand, within RR42_RS02115 at 470.275 kb on - strand, within RR42_RS02115 at 470.288 kb on + strand, within RR42_RS02115 at 470.288 kb on + strand, within RR42_RS02115 at 470.289 kb on - strand, within RR42_RS02115 at 470.289 kb on - strand, within RR42_RS02115 at 470.329 kb on - strand, within RR42_RS02115 at 470.445 kb on - strand, within RR42_RS02115 at 470.518 kb on + strand at 470.518 kb on + strand at 470.518 kb on + strand at 470.519 kb on - strand at 470.588 kb on + strand at 470.588 kb on + strand at 470.589 kb on - strand at 470.593 kb on + strand at 470.594 kb on - strand at 470.711 kb on + strand, within RR42_RS02120 at 470.711 kb on + strand, within RR42_RS02120 at 470.832 kb on + strand, within RR42_RS02120 at 470.832 kb on + strand, within RR42_RS02120 at 470.888 kb on + strand, within RR42_RS02120 at 470.888 kb on + strand, within RR42_RS02120 at 470.991 kb on + strand, within RR42_RS02120 at 470.992 kb on - strand, within RR42_RS02120 at 470.993 kb on + strand, within RR42_RS02120 at 470.993 kb on + strand, within RR42_RS02120 at 470.993 kb on + strand, within RR42_RS02120 at 470.993 kb on + strand, within RR42_RS02120 at 470.993 kb on + strand, within RR42_RS02120 at 470.994 kb on - strand, within RR42_RS02120 at 470.994 kb on - strand, within RR42_RS02120 at 470.994 kb on - strand, within RR42_RS02120 at 471.075 kb on + strand, within RR42_RS02120 at 471.076 kb on - strand, within RR42_RS02120 at 471.076 kb on - strand, within RR42_RS02120 at 471.120 kb on - strand at 471.178 kb on + strand at 471.178 kb on + strand at 471.178 kb on + strand at 471.178 kb on + strand at 471.179 kb on - strand at 471.336 kb on - strand at 471.336 kb on - strand at 471.336 kb on - strand at 471.336 kb on - strand at 471.375 kb on + strand at 471.376 kb on - strand at 471.502 kb on - strand, within RR42_RS02125 at 471.573 kb on + strand, within RR42_RS02125 at 471.573 kb on + strand, within RR42_RS02125 at 471.657 kb on + strand, within RR42_RS02125 at 471.657 kb on + strand, within RR42_RS02125 at 471.657 kb on + strand, within RR42_RS02125 at 471.658 kb on - strand, within RR42_RS02125 at 472.081 kb on - strand at 472.100 kb on + strand at 472.101 kb on - strand at 472.101 kb on - strand at 472.150 kb on + strand at 472.150 kb on + strand at 472.150 kb on + strand at 472.151 kb on - strand at 472.151 kb on - strand at 472.158 kb on + strand at 472.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 469,661 + RR42_RS02110 0.78 -1.5 469,803 + RR42_RS02110 0.85 -0.8 469,822 - RR42_RS02110 0.86 -0.4 469,822 - RR42_RS02110 0.86 -0.0 469,822 - RR42_RS02110 0.86 -0.3 470,105 + +0.2 470,105 + -1.4 470,105 + -0.3 470,105 + -0.4 470,106 - -1.1 470,275 - RR42_RS02115 0.36 +0.3 470,275 - RR42_RS02115 0.36 +1.3 470,288 + RR42_RS02115 0.40 +0.1 470,288 + RR42_RS02115 0.40 +0.2 470,289 - RR42_RS02115 0.40 +0.2 470,289 - RR42_RS02115 0.40 -1.5 470,329 - RR42_RS02115 0.51 +1.2 470,445 - RR42_RS02115 0.81 +0.8 470,518 + -0.0 470,518 + -1.3 470,518 + -0.2 470,519 - -0.4 470,588 + +0.3 470,588 + -0.3 470,589 - -1.9 470,593 + -0.7 470,594 - -0.5 470,711 + RR42_RS02120 0.21 +0.7 470,711 + RR42_RS02120 0.21 -0.7 470,832 + RR42_RS02120 0.42 -0.4 470,832 + RR42_RS02120 0.42 +0.1 470,888 + RR42_RS02120 0.52 -0.3 470,888 + RR42_RS02120 0.52 -0.6 470,991 + RR42_RS02120 0.70 -0.2 470,992 - RR42_RS02120 0.70 -0.1 470,993 + RR42_RS02120 0.70 -0.6 470,993 + RR42_RS02120 0.70 -1.6 470,993 + RR42_RS02120 0.70 +1.2 470,993 + RR42_RS02120 0.70 -1.4 470,993 + RR42_RS02120 0.70 +0.7 470,994 - RR42_RS02120 0.70 -0.2 470,994 - RR42_RS02120 0.70 -0.1 470,994 - RR42_RS02120 0.70 +1.2 471,075 + RR42_RS02120 0.84 -1.7 471,076 - RR42_RS02120 0.84 +0.2 471,076 - RR42_RS02120 0.84 +0.4 471,120 - +1.0 471,178 + +0.5 471,178 + -0.0 471,178 + -0.6 471,178 + +0.3 471,179 - +1.4 471,336 - +1.2 471,336 - +1.0 471,336 - -0.9 471,336 - +0.3 471,375 + +0.6 471,376 - +1.0 471,502 - RR42_RS02125 0.31 -0.1 471,573 + RR42_RS02125 0.43 +0.1 471,573 + RR42_RS02125 0.43 +2.6 471,657 + RR42_RS02125 0.58 +0.8 471,657 + RR42_RS02125 0.58 -3.3 471,657 + RR42_RS02125 0.58 -0.4 471,658 - RR42_RS02125 0.58 -1.1 472,081 - -1.1 472,100 + +0.3 472,101 - -1.1 472,101 - +0.2 472,150 + +0.2 472,150 + +2.2 472,150 + -0.8 472,151 - -1.0 472,151 - -0.8 472,158 + -2.2 472,159 - +0.2
Or see this region's nucleotide sequence