Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01300 and RR42_RS01305 are separated by 6 nucleotides RR42_RS01305 and RR42_RS01310 overlap by 4 nucleotides RR42_RS01310 and RR42_RS01315 are separated by 92 nucleotides RR42_RS01315 and RR42_RS01320 are separated by 59 nucleotides
RR42_RS01300: RR42_RS01300 - thiamine biosynthesis protein ThiS, at 297,091 to 297,288
_RS01300
RR42_RS01305: RR42_RS01305 - thiazole synthase, at 297,295 to 298,143
_RS01305
RR42_RS01310: RR42_RS01310 - thiamine-phosphate pyrophosphorylase, at 298,140 to 299,273
_RS01310
RR42_RS01315: RR42_RS01315 - GNAT family acetyltransferase, at 299,366 to 299,869
_RS01315
RR42_RS01320: RR42_RS01320 - ATPase AAA, at 299,929 to 300,705
_RS01320
Position (kb) 298
299
300 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 299.401 kb on - strand at 299.401 kb on - strand at 299.439 kb on + strand, within RR42_RS01315 at 299.440 kb on - strand, within RR42_RS01315 at 299.440 kb on - strand, within RR42_RS01315 at 300.037 kb on + strand, within RR42_RS01320 at 300.124 kb on + strand, within RR42_RS01320 at 300.124 kb on + strand, within RR42_RS01320 at 300.125 kb on - strand, within RR42_RS01320 at 300.193 kb on + strand, within RR42_RS01320 at 300.193 kb on + strand, within RR42_RS01320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 299,401 - +1.6 299,401 - +3.8 299,439 + RR42_RS01315 0.14 +0.6 299,440 - RR42_RS01315 0.15 +0.2 299,440 - RR42_RS01315 0.15 -0.4 300,037 + RR42_RS01320 0.14 -1.8 300,124 + RR42_RS01320 0.25 +1.1 300,124 + RR42_RS01320 0.25 -0.9 300,125 - RR42_RS01320 0.25 -1.7 300,193 + RR42_RS01320 0.34 +1.2 300,193 + RR42_RS01320 0.34 +0.6
Or see this region's nucleotide sequence