Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS00905

Experiment: R2A with Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS00895 and RR42_RS00900 are separated by 119 nucleotidesRR42_RS00900 and RR42_RS00905 overlap by 4 nucleotidesRR42_RS00905 and RR42_RS00910 overlap by 4 nucleotidesRR42_RS00910 and RR42_RS00915 are separated by 8 nucleotides RR42_RS00895: RR42_RS00895 - acyl-CoA dehydrogenase, at 215,817 to 216,947 _RS00895 RR42_RS00900: RR42_RS00900 - 2-hydroxychromene-2-carboxylate isomerase, at 217,067 to 217,768 _RS00900 RR42_RS00905: RR42_RS00905 - haloacid dehalogenase, at 217,765 to 218,454 _RS00905 RR42_RS00910: RR42_RS00910 - hypothetical protein, at 218,451 to 218,876 _RS00910 RR42_RS00915: RR42_RS00915 - glutathione S-transferase, at 218,885 to 219,559 _RS00915 Position (kb) 217 218 219Strain fitness (log2 ratio) -2 -1 0 1 2at 216.774 kb on + strand, within RR42_RS00895at 216.774 kb on + strand, within RR42_RS00895at 216.775 kb on - strand, within RR42_RS00895at 216.775 kb on - strand, within RR42_RS00895at 216.895 kb on - strandat 216.922 kb on + strandat 216.923 kb on - strandat 216.923 kb on - strandat 217.088 kb on + strandat 217.088 kb on + strandat 217.088 kb on + strandat 217.089 kb on - strandat 217.154 kb on + strand, within RR42_RS00900at 217.250 kb on + strand, within RR42_RS00900at 217.292 kb on - strand, within RR42_RS00900at 217.299 kb on - strand, within RR42_RS00900at 217.730 kb on - strandat 217.899 kb on + strand, within RR42_RS00905at 217.900 kb on - strand, within RR42_RS00905at 218.202 kb on + strand, within RR42_RS00905at 218.203 kb on - strand, within RR42_RS00905at 218.203 kb on - strand, within RR42_RS00905at 218.251 kb on + strand, within RR42_RS00905at 218.251 kb on + strand, within RR42_RS00905at 218.251 kb on + strand, within RR42_RS00905at 218.252 kb on - strand, within RR42_RS00905at 218.310 kb on + strand, within RR42_RS00905at 218.425 kb on - strandat 218.592 kb on + strand, within RR42_RS00910at 218.592 kb on + strand, within RR42_RS00910at 218.592 kb on + strand, within RR42_RS00910at 218.639 kb on + strand, within RR42_RS00910at 218.639 kb on + strand, within RR42_RS00910at 218.640 kb on - strand, within RR42_RS00910at 218.640 kb on - strand, within RR42_RS00910at 218.752 kb on - strand, within RR42_RS00910at 218.800 kb on + strand, within RR42_RS00910at 218.801 kb on - strand, within RR42_RS00910at 218.841 kb on + strandat 218.912 kb on + strandat 218.912 kb on + strandat 218.921 kb on + strandat 218.921 kb on + strandat 218.922 kb on - strandat 218.922 kb on - strandat 218.922 kb on - strandat 218.948 kb on + strandat 218.948 kb on + strandat 218.949 kb on - strandat 218.949 kb on - strandat 218.949 kb on - strandat 219.161 kb on + strand, within RR42_RS00915at 219.162 kb on - strand, within RR42_RS00915at 219.162 kb on - strand, within RR42_RS00915at 219.162 kb on - strand, within RR42_RS00915at 219.162 kb on - strand, within RR42_RS00915at 219.236 kb on + strand, within RR42_RS00915at 219.236 kb on + strand, within RR42_RS00915at 219.248 kb on + strand, within RR42_RS00915at 219.249 kb on - strand, within RR42_RS00915at 219.419 kb on + strand, within RR42_RS00915

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM
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216,774 + RR42_RS00895 0.85 +0.4
216,774 + RR42_RS00895 0.85 -0.1
216,775 - RR42_RS00895 0.85 +0.7
216,775 - RR42_RS00895 0.85 +1.2
216,895 - -0.9
216,922 + +0.1
216,923 - -0.2
216,923 - -0.1
217,088 + +0.5
217,088 + +0.3
217,088 + +0.1
217,089 - +0.2
217,154 + RR42_RS00900 0.12 -0.7
217,250 + RR42_RS00900 0.26 -0.3
217,292 - RR42_RS00900 0.32 +2.2
217,299 - RR42_RS00900 0.33 +0.8
217,730 - +0.4
217,899 + RR42_RS00905 0.19 -0.8
217,900 - RR42_RS00905 0.20 +1.1
218,202 + RR42_RS00905 0.63 -0.4
218,203 - RR42_RS00905 0.63 +1.4
218,203 - RR42_RS00905 0.63 +1.3
218,251 + RR42_RS00905 0.70 +1.5
218,251 + RR42_RS00905 0.70 +0.4
218,251 + RR42_RS00905 0.70 +0.7
218,252 - RR42_RS00905 0.71 -0.1
218,310 + RR42_RS00905 0.79 +0.2
218,425 - +0.8
218,592 + RR42_RS00910 0.33 +0.5
218,592 + RR42_RS00910 0.33 -0.4
218,592 + RR42_RS00910 0.33 -0.8
218,639 + RR42_RS00910 0.44 -1.0
218,639 + RR42_RS00910 0.44 +1.2
218,640 - RR42_RS00910 0.44 -2.1
218,640 - RR42_RS00910 0.44 +0.8
218,752 - RR42_RS00910 0.71 -0.9
218,800 + RR42_RS00910 0.82 +0.5
218,801 - RR42_RS00910 0.82 -1.3
218,841 + +1.5
218,912 + -2.2
218,912 + -0.7
218,921 + -1.1
218,921 + +0.1
218,922 - +0.2
218,922 - +0.2
218,922 - -0.1
218,948 + +0.3
218,948 + -0.1
218,949 - +0.6
218,949 - -0.7
218,949 - -0.4
219,161 + RR42_RS00915 0.41 +1.3
219,162 - RR42_RS00915 0.41 -0.6
219,162 - RR42_RS00915 0.41 -0.7
219,162 - RR42_RS00915 0.41 +2.2
219,162 - RR42_RS00915 0.41 -0.4
219,236 + RR42_RS00915 0.52 -1.9
219,236 + RR42_RS00915 0.52 -0.4
219,248 + RR42_RS00915 0.54 +0.6
219,249 - RR42_RS00915 0.54 +0.4
219,419 + RR42_RS00915 0.79 -1.1

Or see this region's nucleotide sequence