Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00815 and RR42_RS00820 are separated by 313 nucleotides RR42_RS00820 and RR42_RS00825 are separated by 260 nucleotides RR42_RS00825 and RR42_RS00830 are separated by 128 nucleotides RR42_RS00830 and RR42_RS00835 are separated by 91 nucleotides
RR42_RS00815: RR42_RS00815 - hypothetical protein, at 200,180 to 200,596
_RS00815
RR42_RS00820: RR42_RS00820 - hypothetical protein, at 200,910 to 201,233
_RS00820
RR42_RS00825: RR42_RS00825 - arginyl-tRNA synthetase, at 201,494 to 203,287
_RS00825
RR42_RS00830: RR42_RS00830 - cell division protein FtsN, at 203,416 to 204,192
_RS00830
RR42_RS00835: RR42_RS00835 - DSBA oxidoreductase, at 204,284 to 204,922
_RS00835
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 200.527 kb on + strand, within RR42_RS00815 at 200.527 kb on + strand, within RR42_RS00815 at 200.527 kb on + strand, within RR42_RS00815 at 200.648 kb on + strand at 200.672 kb on - strand at 200.839 kb on - strand at 200.865 kb on + strand at 200.876 kb on + strand at 200.881 kb on + strand at 200.881 kb on + strand at 200.881 kb on + strand at 200.882 kb on - strand at 200.889 kb on + strand at 200.911 kb on + strand at 200.912 kb on - strand at 200.912 kb on - strand at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.090 kb on + strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.091 kb on - strand, within RR42_RS00820 at 201.093 kb on - strand, within RR42_RS00820 at 201.237 kb on - strand at 201.237 kb on - strand at 201.237 kb on - strand at 201.237 kb on - strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.264 kb on + strand at 201.265 kb on - strand at 201.265 kb on - strand at 201.265 kb on - strand at 201.367 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.285 kb on + strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.286 kb on - strand at 203.335 kb on + strand at 203.335 kb on + strand at 203.341 kb on + strand at 203.386 kb on + strand at 203.387 kb on - strand at 203.388 kb on + strand at 203.476 kb on - strand at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.539 kb on + strand, within RR42_RS00830 at 203.540 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.542 kb on - strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.957 kb on + strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.958 kb on - strand, within RR42_RS00830 at 203.963 kb on - strand, within RR42_RS00830 at 204.082 kb on + strand, within RR42_RS00830 at 204.083 kb on - strand, within RR42_RS00830 at 204.180 kb on + strand at 204.181 kb on - strand at 204.191 kb on - strand at 204.191 kb on - strand at 204.200 kb on + strand at 204.250 kb on + strand at 204.250 kb on + strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand at 204.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 200,527 + RR42_RS00815 0.83 -2.0 200,527 + RR42_RS00815 0.83 +1.0 200,527 + RR42_RS00815 0.83 -1.5 200,648 + -0.6 200,672 - +0.4 200,839 - -0.5 200,865 + -0.0 200,876 + -0.0 200,881 + -1.0 200,881 + -2.3 200,881 + +1.2 200,882 - -0.6 200,889 + +0.8 200,911 + +0.1 200,912 - +1.2 200,912 - +1.4 201,090 + RR42_RS00820 0.56 +0.2 201,090 + RR42_RS00820 0.56 -0.4 201,090 + RR42_RS00820 0.56 +0.4 201,090 + RR42_RS00820 0.56 -0.8 201,090 + RR42_RS00820 0.56 +0.2 201,090 + RR42_RS00820 0.56 -1.2 201,091 - RR42_RS00820 0.56 +0.3 201,091 - RR42_RS00820 0.56 -1.1 201,091 - RR42_RS00820 0.56 -0.6 201,091 - RR42_RS00820 0.56 -0.2 201,091 - RR42_RS00820 0.56 -0.6 201,093 - RR42_RS00820 0.56 +0.0 201,237 - -1.7 201,237 - +0.6 201,237 - -0.5 201,237 - -0.1 201,264 + -0.1 201,264 + -1.0 201,264 + -1.9 201,264 + +0.6 201,264 + +0.3 201,264 + +0.6 201,265 - -1.0 201,265 - -0.8 201,265 - -0.1 201,367 + +0.4 203,285 + +1.4 203,285 + +0.7 203,285 + +0.4 203,285 + +1.2 203,286 - +0.6 203,286 - -2.7 203,286 - +0.4 203,286 - +1.0 203,335 + +0.6 203,335 + -0.7 203,341 + -1.8 203,386 + -1.3 203,387 - +1.1 203,388 + +0.6 203,476 - -2.1 203,539 + RR42_RS00830 0.16 +1.2 203,539 + RR42_RS00830 0.16 -0.4 203,539 + RR42_RS00830 0.16 -0.6 203,539 + RR42_RS00830 0.16 -3.1 203,540 - RR42_RS00830 0.16 -1.0 203,542 - RR42_RS00830 0.16 -2.4 203,542 - RR42_RS00830 0.16 -3.3 203,542 - RR42_RS00830 0.16 -1.8 203,957 + RR42_RS00830 0.70 +0.2 203,957 + RR42_RS00830 0.70 -0.9 203,957 + RR42_RS00830 0.70 -1.4 203,958 - RR42_RS00830 0.70 -1.0 203,958 - RR42_RS00830 0.70 +0.2 203,963 - RR42_RS00830 0.70 +0.6 204,082 + RR42_RS00830 0.86 -1.2 204,083 - RR42_RS00830 0.86 -0.8 204,180 + +0.2 204,181 - +0.2 204,191 - +1.1 204,191 - +2.2 204,200 + +0.6 204,250 + +0.4 204,250 + -1.5 204,251 - -0.2 204,251 - +1.5 204,251 - -0.4 204,251 - -1.4 204,251 - +0.2 204,251 - -1.4 204,251 - -0.7 204,251 - -0.4
Or see this region's nucleotide sequence