Experiment: R2A with Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00735 and RR42_RS00740 are separated by 66 nucleotides RR42_RS00740 and RR42_RS00745 are separated by 340 nucleotides
RR42_RS00735: RR42_RS00735 - fumarylacetoacetate hydrolase, at 185,200 to 186,183
_RS00735
RR42_RS00740: RR42_RS00740 - ABC transporter substrate-binding protein, at 186,250 to 187,206
_RS00740
RR42_RS00745: RR42_RS00745 - IclR family transcriptional regulator, at 187,547 to 188,383
_RS00745
Position (kb)
186
187
188 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 185.360 kb on + strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.440 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.585 kb on - strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.668 kb on + strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.669 kb on - strand, within RR42_RS00735 at 185.803 kb on - strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.843 kb on + strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 185.844 kb on - strand, within RR42_RS00735 at 186.026 kb on + strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.027 kb on - strand, within RR42_RS00735 at 186.104 kb on + strand at 186.104 kb on + strand at 186.105 kb on - strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.185 kb on + strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.186 kb on - strand at 186.197 kb on + strand at 186.252 kb on - strand at 186.476 kb on + strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.477 kb on - strand, within RR42_RS00740 at 186.672 kb on - strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.788 kb on + strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.789 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.897 kb on - strand, within RR42_RS00740 at 186.911 kb on + strand, within RR42_RS00740 at 186.962 kb on + strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 186.963 kb on - strand, within RR42_RS00740 at 187.202 kb on - strand at 187.286 kb on - strand at 187.358 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.378 kb on + strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.379 kb on - strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.380 kb on + strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.381 kb on - strand at 187.444 kb on + strand at 187.445 kb on - strand at 187.445 kb on - strand at 187.446 kb on + strand at 187.450 kb on - strand at 187.450 kb on - strand at 187.498 kb on - strand at 187.498 kb on - strand at 187.507 kb on + strand at 187.507 kb on + strand at 187.508 kb on - strand at 187.508 kb on - strand at 187.706 kb on + strand, within RR42_RS00745 at 187.764 kb on - strand, within RR42_RS00745 at 188.015 kb on + strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.016 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.081 kb on - strand, within RR42_RS00745 at 188.111 kb on + strand at 188.111 kb on + strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745 at 188.112 kb on - strand, within RR42_RS00745
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.5 mM remove 185,360 + RR42_RS00735 0.16 +0.3 185,440 - RR42_RS00735 0.24 -1.0 185,440 - RR42_RS00735 0.24 -2.1 185,585 - RR42_RS00735 0.39 +0.3 185,585 - RR42_RS00735 0.39 -0.2 185,668 + RR42_RS00735 0.48 +0.9 185,668 + RR42_RS00735 0.48 -0.5 185,668 + RR42_RS00735 0.48 -1.3 185,668 + RR42_RS00735 0.48 +1.3 185,668 + RR42_RS00735 0.48 -1.2 185,669 - RR42_RS00735 0.48 -0.7 185,669 - RR42_RS00735 0.48 +0.0 185,669 - RR42_RS00735 0.48 +1.8 185,669 - RR42_RS00735 0.48 +0.0 185,803 - RR42_RS00735 0.61 +1.3 185,843 + RR42_RS00735 0.65 +0.4 185,843 + RR42_RS00735 0.65 +0.4 185,843 + RR42_RS00735 0.65 -0.8 185,844 - RR42_RS00735 0.65 +0.2 185,844 - RR42_RS00735 0.65 +0.3 185,844 - RR42_RS00735 0.65 +0.9 185,844 - RR42_RS00735 0.65 +0.4 186,026 + RR42_RS00735 0.84 -0.4 186,027 - RR42_RS00735 0.84 -0.4 186,027 - RR42_RS00735 0.84 +0.1 186,027 - RR42_RS00735 0.84 -0.8 186,104 + -0.5 186,104 + +0.2 186,105 - -0.9 186,185 + +2.3 186,185 + +0.6 186,185 + +2.0 186,185 + -0.4 186,185 + +0.4 186,185 + +1.0 186,186 - -0.2 186,186 - -0.4 186,186 - -0.8 186,186 - -0.2 186,186 - +0.3 186,197 + +0.0 186,252 - +0.3 186,476 + RR42_RS00740 0.24 +0.6 186,477 - RR42_RS00740 0.24 -0.4 186,477 - RR42_RS00740 0.24 +0.2 186,477 - RR42_RS00740 0.24 +1.5 186,672 - RR42_RS00740 0.44 +0.2 186,788 + RR42_RS00740 0.56 +0.3 186,788 + RR42_RS00740 0.56 -1.1 186,788 + RR42_RS00740 0.56 +0.2 186,788 + RR42_RS00740 0.56 -1.0 186,789 - RR42_RS00740 0.56 +0.8 186,789 - RR42_RS00740 0.56 -0.7 186,789 - RR42_RS00740 0.56 -0.6 186,897 - RR42_RS00740 0.68 +0.9 186,897 - RR42_RS00740 0.68 +1.6 186,911 + RR42_RS00740 0.69 -0.5 186,962 + RR42_RS00740 0.74 +0.8 186,963 - RR42_RS00740 0.75 +1.6 186,963 - RR42_RS00740 0.75 -1.5 186,963 - RR42_RS00740 0.75 +0.8 187,202 - -0.4 187,286 - -0.2 187,358 + -0.6 187,378 + +1.2 187,378 + -0.2 187,378 + +0.1 187,378 + +1.1 187,378 + -1.2 187,378 + +0.5 187,378 + +2.2 187,378 + -0.4 187,379 - +1.2 187,379 - -0.0 187,379 - -0.8 187,379 - -0.1 187,380 + -1.9 187,380 + +0.7 187,380 + +1.7 187,380 + +0.5 187,380 + -0.6 187,380 + +0.9 187,380 + +0.2 187,380 + +1.2 187,380 + +0.9 187,380 + +1.4 187,380 + -0.2 187,380 + +3.4 187,381 - -0.5 187,381 - -0.4 187,381 - -0.1 187,381 - -0.7 187,381 - +0.5 187,381 - +0.0 187,381 - -0.8 187,381 - +0.3 187,381 - +2.2 187,444 + +1.2 187,445 - -2.4 187,445 - +0.8 187,446 + +0.1 187,450 - -0.8 187,450 - +0.2 187,498 - -1.3 187,498 - -0.0 187,507 + +3.8 187,507 + +1.4 187,508 - -0.0 187,508 - +1.1 187,706 + RR42_RS00745 0.19 -1.4 187,764 - RR42_RS00745 0.26 +0.6 188,015 + RR42_RS00745 0.56 -0.0 188,016 - RR42_RS00745 0.56 -2.1 188,016 - RR42_RS00745 0.56 -1.2 188,081 - RR42_RS00745 0.64 +1.8 188,081 - RR42_RS00745 0.64 -2.6 188,081 - RR42_RS00745 0.64 -0.5 188,081 - RR42_RS00745 0.64 -0.3 188,111 + +0.6 188,111 + RR42_RS00745 0.67 -2.3 188,112 - RR42_RS00745 0.68 -0.9 188,112 - RR42_RS00745 0.68 +0.1
Or see this region's nucleotide sequence