Experiment: R2A with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12745 and CA265_RS12750 are separated by 435 nucleotides CA265_RS12750 and CA265_RS12755 are separated by 94 nucleotides
CA265_RS12745: CA265_RS12745 - NUDIX hydrolase, at 3,175,173 to 3,175,784
_RS12745
CA265_RS12750: CA265_RS12750 - hypothetical protein, at 3,176,220 to 3,177,086
_RS12750
CA265_RS12755: CA265_RS12755 - hydroxyacid dehydrogenase, at 3,177,181 to 3,178,224
_RS12755
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3175.226 kb on + strand at 3175.227 kb on - strand at 3175.356 kb on - strand, within CA265_RS12745 at 3175.407 kb on - strand, within CA265_RS12745 at 3175.517 kb on + strand, within CA265_RS12745 at 3175.525 kb on + strand, within CA265_RS12745 at 3175.526 kb on - strand, within CA265_RS12745 at 3175.568 kb on + strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.575 kb on - strand, within CA265_RS12745 at 3175.629 kb on - strand, within CA265_RS12745 at 3175.762 kb on + strand at 3175.802 kb on - strand at 3175.831 kb on + strand at 3175.832 kb on - strand at 3175.875 kb on + strand at 3175.876 kb on - strand at 3175.876 kb on - strand at 3175.877 kb on + strand at 3175.879 kb on + strand at 3175.879 kb on + strand at 3175.901 kb on - strand at 3175.921 kb on + strand at 3175.922 kb on - strand at 3175.927 kb on + strand at 3175.931 kb on - strand at 3175.950 kb on + strand at 3175.958 kb on + strand at 3175.964 kb on + strand at 3175.968 kb on + strand at 3176.035 kb on - strand at 3176.083 kb on + strand at 3176.108 kb on - strand at 3176.131 kb on - strand at 3176.259 kb on + strand at 3176.353 kb on + strand, within CA265_RS12750 at 3176.354 kb on - strand, within CA265_RS12750 at 3176.403 kb on + strand, within CA265_RS12750 at 3176.424 kb on + strand, within CA265_RS12750 at 3176.424 kb on + strand, within CA265_RS12750 at 3176.429 kb on + strand, within CA265_RS12750 at 3176.447 kb on + strand, within CA265_RS12750 at 3176.500 kb on + strand, within CA265_RS12750 at 3176.543 kb on + strand, within CA265_RS12750 at 3176.543 kb on + strand, within CA265_RS12750 at 3176.544 kb on - strand, within CA265_RS12750 at 3176.609 kb on - strand, within CA265_RS12750 at 3176.627 kb on - strand, within CA265_RS12750 at 3176.630 kb on + strand, within CA265_RS12750 at 3176.876 kb on + strand, within CA265_RS12750 at 3176.877 kb on - strand, within CA265_RS12750 at 3176.950 kb on + strand, within CA265_RS12750 at 3176.951 kb on - strand, within CA265_RS12750 at 3176.951 kb on - strand, within CA265_RS12750 at 3176.954 kb on + strand, within CA265_RS12750 at 3176.961 kb on + strand, within CA265_RS12750 at 3176.981 kb on + strand, within CA265_RS12750 at 3176.981 kb on + strand, within CA265_RS12750 at 3176.981 kb on + strand, within CA265_RS12750 at 3177.076 kb on - strand at 3177.131 kb on - strand at 3177.131 kb on - strand at 3177.143 kb on + strand at 3177.152 kb on - strand at 3177.183 kb on - strand at 3177.184 kb on + strand at 3177.184 kb on + strand at 3177.196 kb on - strand at 3177.264 kb on + strand at 3177.265 kb on - strand at 3177.379 kb on + strand, within CA265_RS12755 at 3177.422 kb on - strand, within CA265_RS12755 at 3177.461 kb on + strand, within CA265_RS12755 at 3177.461 kb on + strand, within CA265_RS12755 at 3177.461 kb on + strand, within CA265_RS12755 at 3177.513 kb on + strand, within CA265_RS12755 at 3177.683 kb on - strand, within CA265_RS12755 at 3177.694 kb on - strand, within CA265_RS12755 at 3177.721 kb on + strand, within CA265_RS12755 at 3177.721 kb on + strand, within CA265_RS12755 at 3177.731 kb on + strand, within CA265_RS12755 at 3177.731 kb on + strand, within CA265_RS12755 at 3177.732 kb on - strand, within CA265_RS12755 at 3177.732 kb on - strand, within CA265_RS12755 at 3177.738 kb on + strand, within CA265_RS12755 at 3177.751 kb on + strand, within CA265_RS12755 at 3177.751 kb on + strand, within CA265_RS12755 at 3177.752 kb on - strand, within CA265_RS12755 at 3177.784 kb on - strand, within CA265_RS12755 at 3177.818 kb on - strand, within CA265_RS12755 at 3177.843 kb on - strand, within CA265_RS12755 at 3177.856 kb on - strand, within CA265_RS12755 at 3177.878 kb on + strand, within CA265_RS12755 at 3177.878 kb on + strand, within CA265_RS12755 at 3177.878 kb on + strand, within CA265_RS12755 at 3177.879 kb on - strand, within CA265_RS12755 at 3177.899 kb on - strand, within CA265_RS12755 at 3177.952 kb on + strand, within CA265_RS12755 at 3177.957 kb on + strand, within CA265_RS12755 at 3177.958 kb on - strand, within CA265_RS12755 at 3177.958 kb on - strand, within CA265_RS12755 at 3177.987 kb on + strand, within CA265_RS12755 at 3177.987 kb on + strand, within CA265_RS12755 at 3177.988 kb on - strand, within CA265_RS12755 at 3178.018 kb on + strand, within CA265_RS12755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.04 mM remove 3,175,226 + -0.7 3,175,227 - -0.9 3,175,356 - CA265_RS12745 0.30 +0.1 3,175,407 - CA265_RS12745 0.38 +0.2 3,175,517 + CA265_RS12745 0.56 +1.5 3,175,525 + CA265_RS12745 0.58 +0.3 3,175,526 - CA265_RS12745 0.58 +1.4 3,175,568 + CA265_RS12745 0.65 +1.0 3,175,575 - CA265_RS12745 0.66 -2.0 3,175,575 - CA265_RS12745 0.66 +1.1 3,175,575 - CA265_RS12745 0.66 -0.5 3,175,629 - CA265_RS12745 0.75 +0.5 3,175,762 + +0.3 3,175,802 - +0.3 3,175,831 + +0.7 3,175,832 - -1.2 3,175,875 + -0.2 3,175,876 - +1.7 3,175,876 - +0.2 3,175,877 + -0.2 3,175,879 + -0.1 3,175,879 + +0.7 3,175,901 - +0.4 3,175,921 + -1.2 3,175,922 - +0.1 3,175,927 + -1.6 3,175,931 - -0.3 3,175,950 + -0.3 3,175,958 + -1.4 3,175,964 + +0.1 3,175,968 + +1.3 3,176,035 - -0.6 3,176,083 + -0.2 3,176,108 - +0.1 3,176,131 - +0.7 3,176,259 + -0.1 3,176,353 + CA265_RS12750 0.15 +3.1 3,176,354 - CA265_RS12750 0.15 +0.6 3,176,403 + CA265_RS12750 0.21 +1.4 3,176,424 + CA265_RS12750 0.24 +0.7 3,176,424 + CA265_RS12750 0.24 +0.8 3,176,429 + CA265_RS12750 0.24 +0.6 3,176,447 + CA265_RS12750 0.26 +0.3 3,176,500 + CA265_RS12750 0.32 +1.5 3,176,543 + CA265_RS12750 0.37 +0.5 3,176,543 + CA265_RS12750 0.37 +0.2 3,176,544 - CA265_RS12750 0.37 +0.6 3,176,609 - CA265_RS12750 0.45 +1.3 3,176,627 - CA265_RS12750 0.47 +0.2 3,176,630 + CA265_RS12750 0.47 +0.4 3,176,876 + CA265_RS12750 0.76 -0.2 3,176,877 - CA265_RS12750 0.76 +0.2 3,176,950 + CA265_RS12750 0.84 +0.1 3,176,951 - CA265_RS12750 0.84 +0.3 3,176,951 - CA265_RS12750 0.84 -0.4 3,176,954 + CA265_RS12750 0.85 +0.3 3,176,961 + CA265_RS12750 0.85 -0.2 3,176,981 + CA265_RS12750 0.88 -0.6 3,176,981 + CA265_RS12750 0.88 -0.7 3,176,981 + CA265_RS12750 0.88 -0.1 3,177,076 - +0.2 3,177,131 - -0.3 3,177,131 - +1.6 3,177,143 + -0.3 3,177,152 - -0.4 3,177,183 - -0.3 3,177,184 + -0.2 3,177,184 + -0.4 3,177,196 - +0.1 3,177,264 + +0.1 3,177,265 - -2.0 3,177,379 + CA265_RS12755 0.19 -0.6 3,177,422 - CA265_RS12755 0.23 -1.2 3,177,461 + CA265_RS12755 0.27 -0.7 3,177,461 + CA265_RS12755 0.27 +1.0 3,177,461 + CA265_RS12755 0.27 +1.1 3,177,513 + CA265_RS12755 0.32 -0.2 3,177,683 - CA265_RS12755 0.48 -0.3 3,177,694 - CA265_RS12755 0.49 +0.4 3,177,721 + CA265_RS12755 0.52 -0.1 3,177,721 + CA265_RS12755 0.52 +0.4 3,177,731 + CA265_RS12755 0.53 +0.2 3,177,731 + CA265_RS12755 0.53 +0.6 3,177,732 - CA265_RS12755 0.53 -0.4 3,177,732 - CA265_RS12755 0.53 +0.1 3,177,738 + CA265_RS12755 0.53 -0.2 3,177,751 + CA265_RS12755 0.55 +0.0 3,177,751 + CA265_RS12755 0.55 +0.4 3,177,752 - CA265_RS12755 0.55 -0.1 3,177,784 - CA265_RS12755 0.58 +0.5 3,177,818 - CA265_RS12755 0.61 -0.5 3,177,843 - CA265_RS12755 0.63 -0.7 3,177,856 - CA265_RS12755 0.65 -0.1 3,177,878 + CA265_RS12755 0.67 +0.4 3,177,878 + CA265_RS12755 0.67 -0.0 3,177,878 + CA265_RS12755 0.67 +0.7 3,177,879 - CA265_RS12755 0.67 -0.9 3,177,899 - CA265_RS12755 0.69 -0.9 3,177,952 + CA265_RS12755 0.74 +0.1 3,177,957 + CA265_RS12755 0.74 +0.0 3,177,958 - CA265_RS12755 0.74 -0.4 3,177,958 - CA265_RS12755 0.74 +0.3 3,177,987 + CA265_RS12755 0.77 -0.3 3,177,987 + CA265_RS12755 0.77 -0.6 3,177,988 - CA265_RS12755 0.77 +0.2 3,178,018 + CA265_RS12755 0.80 +1.1
Or see this region's nucleotide sequence