Experiment: R2A with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07520 and CA265_RS07525 overlap by 14 nucleotides CA265_RS07525 and CA265_RS07530 are separated by 8 nucleotides CA265_RS07530 and CA265_RS07535 are separated by 87 nucleotides CA265_RS07535 and CA265_RS07540 are separated by 2 nucleotides CA265_RS07540 and CA265_RS07545 are separated by 87 nucleotides
CA265_RS07520: CA265_RS07520 - cation diffusion facilitator family transporter, at 1,895,023 to 1,896,009
_RS07520
CA265_RS07525: CA265_RS07525 - NUDIX hydrolase, at 1,895,996 to 1,896,448
_RS07525
CA265_RS07530: CA265_RS07530 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,896,457 to 1,897,758
_RS07530
CA265_RS07535: CA265_RS07535 - ATPase, at 1,897,846 to 1,898,280
_RS07535
CA265_RS07540: CA265_RS07540 - transcriptional regulator, at 1,898,283 to 1,898,600
_RS07540
CA265_RS07545: CA265_RS07545 - extradiol dioxygenase, at 1,898,688 to 1,899,104
_RS07545
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1895.482 kb on + strand, within CA265_RS07520 at 1895.513 kb on + strand, within CA265_RS07520 at 1895.532 kb on + strand, within CA265_RS07520 at 1895.533 kb on - strand, within CA265_RS07520 at 1895.552 kb on + strand, within CA265_RS07520 at 1895.553 kb on - strand, within CA265_RS07520 at 1895.575 kb on + strand, within CA265_RS07520 at 1895.596 kb on - strand, within CA265_RS07520 at 1895.729 kb on - strand, within CA265_RS07520 at 1895.748 kb on - strand, within CA265_RS07520 at 1895.855 kb on + strand, within CA265_RS07520 at 1895.904 kb on + strand, within CA265_RS07520 at 1895.967 kb on - strand at 1895.970 kb on - strand at 1896.007 kb on + strand at 1896.007 kb on + strand at 1896.030 kb on + strand at 1896.030 kb on + strand at 1896.043 kb on + strand, within CA265_RS07525 at 1896.075 kb on - strand, within CA265_RS07525 at 1896.075 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.198 kb on - strand, within CA265_RS07525 at 1896.198 kb on - strand, within CA265_RS07525 at 1896.199 kb on + strand, within CA265_RS07525 at 1896.202 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.255 kb on - strand, within CA265_RS07525 at 1896.282 kb on + strand, within CA265_RS07525 at 1896.302 kb on - strand, within CA265_RS07525 at 1896.369 kb on + strand, within CA265_RS07525 at 1896.373 kb on + strand, within CA265_RS07525 at 1896.446 kb on + strand at 1896.504 kb on - strand at 1896.524 kb on + strand at 1896.525 kb on - strand at 1896.596 kb on - strand, within CA265_RS07530 at 1896.624 kb on + strand, within CA265_RS07530 at 1896.625 kb on - strand, within CA265_RS07530 at 1896.655 kb on - strand, within CA265_RS07530 at 1896.655 kb on - strand, within CA265_RS07530 at 1896.679 kb on - strand, within CA265_RS07530 at 1896.758 kb on + strand, within CA265_RS07530 at 1896.759 kb on - strand, within CA265_RS07530 at 1896.919 kb on + strand, within CA265_RS07530 at 1897.033 kb on - strand, within CA265_RS07530 at 1897.138 kb on + strand, within CA265_RS07530 at 1897.182 kb on - strand, within CA265_RS07530 at 1897.185 kb on + strand, within CA265_RS07530 at 1897.200 kb on - strand, within CA265_RS07530 at 1897.228 kb on + strand, within CA265_RS07530 at 1897.228 kb on + strand, within CA265_RS07530 at 1897.252 kb on - strand, within CA265_RS07530 at 1897.252 kb on - strand, within CA265_RS07530 at 1897.437 kb on + strand, within CA265_RS07530 at 1897.513 kb on - strand, within CA265_RS07530 at 1897.599 kb on - strand, within CA265_RS07530 at 1897.602 kb on + strand, within CA265_RS07530 at 1897.649 kb on + strand at 1897.650 kb on - strand at 1897.717 kb on + strand at 1897.723 kb on + strand at 1897.724 kb on - strand at 1897.756 kb on + strand at 1897.756 kb on + strand at 1897.757 kb on - strand at 1897.757 kb on - strand at 1897.757 kb on - strand at 1897.761 kb on - strand at 1897.761 kb on - strand at 1897.761 kb on - strand at 1897.777 kb on + strand at 1897.816 kb on - strand at 1897.892 kb on + strand, within CA265_RS07535 at 1898.013 kb on + strand at 1898.042 kb on + strand, within CA265_RS07535 at 1898.105 kb on + strand, within CA265_RS07535 at 1898.106 kb on - strand, within CA265_RS07535 at 1898.262 kb on + strand at 1898.262 kb on + strand at 1898.329 kb on - strand, within CA265_RS07540 at 1898.402 kb on + strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.528 kb on + strand, within CA265_RS07540 at 1898.564 kb on + strand, within CA265_RS07540 at 1898.640 kb on - strand at 1898.683 kb on - strand at 1898.694 kb on + strand at 1898.695 kb on - strand at 1898.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.04 mM remove 1,895,482 + CA265_RS07520 0.47 -0.1 1,895,513 + CA265_RS07520 0.50 +0.1 1,895,532 + CA265_RS07520 0.52 +0.8 1,895,533 - CA265_RS07520 0.52 +0.3 1,895,552 + CA265_RS07520 0.54 -0.3 1,895,553 - CA265_RS07520 0.54 +0.5 1,895,575 + CA265_RS07520 0.56 -0.6 1,895,596 - CA265_RS07520 0.58 +0.4 1,895,729 - CA265_RS07520 0.72 +0.3 1,895,748 - CA265_RS07520 0.73 +0.0 1,895,855 + CA265_RS07520 0.84 -0.1 1,895,904 + CA265_RS07520 0.89 -0.3 1,895,967 - -0.2 1,895,970 - +0.2 1,896,007 + +0.2 1,896,007 + -0.1 1,896,030 + -0.9 1,896,030 + -1.2 1,896,043 + CA265_RS07525 0.10 +0.7 1,896,075 - CA265_RS07525 0.17 +0.0 1,896,075 - CA265_RS07525 0.17 -1.8 1,896,187 - CA265_RS07525 0.42 +0.0 1,896,187 - CA265_RS07525 0.42 +0.5 1,896,187 - CA265_RS07525 0.42 -0.3 1,896,198 - CA265_RS07525 0.45 +0.5 1,896,198 - CA265_RS07525 0.45 +0.0 1,896,199 + CA265_RS07525 0.45 +0.9 1,896,202 + CA265_RS07525 0.45 -1.0 1,896,254 + CA265_RS07525 0.57 -0.3 1,896,254 + CA265_RS07525 0.57 -0.7 1,896,254 + CA265_RS07525 0.57 +0.0 1,896,255 - CA265_RS07525 0.57 -0.1 1,896,282 + CA265_RS07525 0.63 +0.2 1,896,302 - CA265_RS07525 0.68 -0.3 1,896,369 + CA265_RS07525 0.82 -0.6 1,896,373 + CA265_RS07525 0.83 -0.2 1,896,446 + -1.5 1,896,504 - -0.2 1,896,524 + -0.3 1,896,525 - +0.0 1,896,596 - CA265_RS07530 0.11 -0.1 1,896,624 + CA265_RS07530 0.13 -0.1 1,896,625 - CA265_RS07530 0.13 -0.1 1,896,655 - CA265_RS07530 0.15 -0.1 1,896,655 - CA265_RS07530 0.15 -0.6 1,896,679 - CA265_RS07530 0.17 +0.1 1,896,758 + CA265_RS07530 0.23 -1.7 1,896,759 - CA265_RS07530 0.23 +0.8 1,896,919 + CA265_RS07530 0.35 +0.2 1,897,033 - CA265_RS07530 0.44 -0.4 1,897,138 + CA265_RS07530 0.52 +0.3 1,897,182 - CA265_RS07530 0.56 +1.7 1,897,185 + CA265_RS07530 0.56 -0.3 1,897,200 - CA265_RS07530 0.57 -0.7 1,897,228 + CA265_RS07530 0.59 +0.7 1,897,228 + CA265_RS07530 0.59 +0.3 1,897,252 - CA265_RS07530 0.61 +0.2 1,897,252 - CA265_RS07530 0.61 -0.9 1,897,437 + CA265_RS07530 0.75 +0.4 1,897,513 - CA265_RS07530 0.81 +0.1 1,897,599 - CA265_RS07530 0.88 +0.2 1,897,602 + CA265_RS07530 0.88 -0.3 1,897,649 + -0.1 1,897,650 - -0.2 1,897,717 + -0.8 1,897,723 + -0.4 1,897,724 - -0.5 1,897,756 + -1.2 1,897,756 + -1.0 1,897,757 - -0.5 1,897,757 - +0.9 1,897,757 - +1.7 1,897,761 - -0.1 1,897,761 - -0.1 1,897,761 - -0.2 1,897,777 + +0.2 1,897,816 - +0.5 1,897,892 + CA265_RS07535 0.11 -0.3 1,898,013 + +0.9 1,898,042 + CA265_RS07535 0.45 +0.1 1,898,105 + CA265_RS07535 0.60 +0.1 1,898,106 - CA265_RS07535 0.60 -0.3 1,898,262 + -0.6 1,898,262 + +0.2 1,898,329 - CA265_RS07540 0.14 -0.8 1,898,402 + CA265_RS07540 0.37 -0.4 1,898,403 - CA265_RS07540 0.38 +1.1 1,898,403 - CA265_RS07540 0.38 -0.7 1,898,403 - CA265_RS07540 0.38 -0.9 1,898,528 + CA265_RS07540 0.77 -1.1 1,898,564 + CA265_RS07540 0.88 -0.2 1,898,640 - +0.8 1,898,683 - -2.9 1,898,694 + -0.1 1,898,695 - -1.4 1,898,717 + +0.0
Or see this region's nucleotide sequence