Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35880 and RR42_RS35885 are separated by 316 nucleotides RR42_RS35885 and RR42_RS35890 are separated by 60 nucleotides RR42_RS35890 and RR42_RS35895 are separated by 129 nucleotides RR42_RS35895 and RR42_RS35900 are separated by 311 nucleotides
RR42_RS35880: RR42_RS35880 - integrase, at 3,498,037 to 3,499,017
_RS35880
RR42_RS35885: RR42_RS35885 - membrane protein, at 3,499,334 to 3,499,771
_RS35885
RR42_RS35890: RR42_RS35890 - 2-nitropropane dioxygenase, at 3,499,832 to 3,500,833
_RS35890
RR42_RS35895: RR42_RS35895 - PadR family transcriptional regulator, at 3,500,963 to 3,501,508
_RS35895
RR42_RS35900: RR42_RS35900 - hypothetical protein, at 3,501,820 to 3,502,041
_RS35900
Position (kb)
3499
3500
3501 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3498.878 kb on + strand, within RR42_RS35880 at 3499.012 kb on - strand at 3499.012 kb on - strand at 3499.051 kb on + strand at 3499.052 kb on - strand at 3499.052 kb on - strand at 3499.056 kb on + strand at 3499.131 kb on + strand at 3499.189 kb on + strand at 3499.189 kb on + strand at 3499.190 kb on - strand at 3499.232 kb on + strand at 3499.232 kb on + strand at 3499.233 kb on - strand at 3499.233 kb on - strand at 3499.233 kb on - strand at 3499.375 kb on - strand at 3499.642 kb on + strand, within RR42_RS35885 at 3499.790 kb on + strand at 3499.790 kb on + strand at 3499.790 kb on + strand at 3499.797 kb on - strand at 3499.810 kb on + strand at 3499.810 kb on + strand at 3499.810 kb on + strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3499.811 kb on - strand at 3500.132 kb on + strand, within RR42_RS35890 at 3500.132 kb on + strand, within RR42_RS35890 at 3500.132 kb on + strand, within RR42_RS35890 at 3500.133 kb on - strand, within RR42_RS35890 at 3500.133 kb on - strand, within RR42_RS35890 at 3500.144 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.199 kb on - strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.438 kb on + strand, within RR42_RS35890 at 3500.439 kb on - strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.531 kb on + strand, within RR42_RS35890 at 3500.532 kb on - strand, within RR42_RS35890 at 3500.546 kb on - strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.564 kb on + strand, within RR42_RS35890 at 3500.565 kb on - strand, within RR42_RS35890 at 3500.713 kb on - strand, within RR42_RS35890 at 3500.753 kb on + strand at 3500.754 kb on - strand at 3500.754 kb on - strand at 3500.798 kb on + strand at 3500.799 kb on - strand at 3500.895 kb on + strand at 3500.895 kb on + strand at 3500.983 kb on + strand at 3501.033 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.123 kb on + strand, within RR42_RS35895 at 3501.124 kb on - strand, within RR42_RS35895 at 3501.124 kb on - strand, within RR42_RS35895 at 3501.304 kb on - strand, within RR42_RS35895 at 3501.418 kb on - strand, within RR42_RS35895 at 3501.550 kb on + strand at 3501.590 kb on + strand at 3501.697 kb on - strand at 3501.697 kb on - strand at 3501.710 kb on + strand at 3501.711 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.734 kb on - strand at 3501.747 kb on + strand at 3501.747 kb on + strand at 3501.747 kb on + strand at 3501.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,498,878 + RR42_RS35880 0.86 +2.6 3,499,012 - +0.1 3,499,012 - -1.0 3,499,051 + +0.7 3,499,052 - -0.9 3,499,052 - -0.3 3,499,056 + -0.3 3,499,131 + -0.3 3,499,189 + -0.3 3,499,189 + +0.8 3,499,190 - -0.2 3,499,232 + +0.7 3,499,232 + +0.3 3,499,233 - -2.2 3,499,233 - -0.7 3,499,233 - +0.4 3,499,375 - +0.5 3,499,642 + RR42_RS35885 0.70 +0.8 3,499,790 + -0.5 3,499,790 + -1.3 3,499,790 + +0.7 3,499,797 - -1.5 3,499,810 + +0.6 3,499,810 + -0.4 3,499,810 + -0.0 3,499,811 - -0.7 3,499,811 - -0.4 3,499,811 - +0.5 3,499,811 - +0.1 3,499,811 - -2.1 3,500,132 + RR42_RS35890 0.30 -0.8 3,500,132 + RR42_RS35890 0.30 -0.3 3,500,132 + RR42_RS35890 0.30 -0.4 3,500,133 - RR42_RS35890 0.30 -0.9 3,500,133 - RR42_RS35890 0.30 -1.5 3,500,144 - RR42_RS35890 0.31 -0.2 3,500,199 - RR42_RS35890 0.37 +0.2 3,500,199 - RR42_RS35890 0.37 -1.5 3,500,199 - RR42_RS35890 0.37 -0.1 3,500,438 + RR42_RS35890 0.60 -0.5 3,500,438 + RR42_RS35890 0.60 -1.0 3,500,438 + RR42_RS35890 0.60 +0.3 3,500,438 + RR42_RS35890 0.60 -1.3 3,500,439 - RR42_RS35890 0.61 +1.6 3,500,531 + RR42_RS35890 0.70 -0.3 3,500,531 + RR42_RS35890 0.70 +1.1 3,500,531 + RR42_RS35890 0.70 +0.8 3,500,532 - RR42_RS35890 0.70 -0.4 3,500,546 - RR42_RS35890 0.71 +0.3 3,500,564 + RR42_RS35890 0.73 +0.0 3,500,564 + RR42_RS35890 0.73 +0.9 3,500,564 + RR42_RS35890 0.73 +1.4 3,500,564 + RR42_RS35890 0.73 -0.2 3,500,564 + RR42_RS35890 0.73 +0.1 3,500,564 + RR42_RS35890 0.73 -0.9 3,500,565 - RR42_RS35890 0.73 -0.3 3,500,713 - RR42_RS35890 0.88 -0.7 3,500,753 + -0.6 3,500,754 - -0.5 3,500,754 - -0.2 3,500,798 + -1.3 3,500,799 - -1.6 3,500,895 + -0.1 3,500,895 + -0.4 3,500,983 + +0.1 3,501,033 + RR42_RS35895 0.13 -1.7 3,501,123 + RR42_RS35895 0.29 +1.0 3,501,123 + RR42_RS35895 0.29 -0.5 3,501,123 + RR42_RS35895 0.29 -0.9 3,501,123 + RR42_RS35895 0.29 +0.3 3,501,124 - RR42_RS35895 0.29 -0.4 3,501,124 - RR42_RS35895 0.29 -0.6 3,501,304 - RR42_RS35895 0.62 +1.6 3,501,418 - RR42_RS35895 0.83 -0.7 3,501,550 + -0.4 3,501,590 + +0.7 3,501,697 - -1.7 3,501,697 - +1.0 3,501,710 + +1.2 3,501,711 - -1.2 3,501,734 - -2.2 3,501,734 - -0.6 3,501,734 - -1.2 3,501,734 - -0.6 3,501,747 + +0.4 3,501,747 + +0.2 3,501,747 + -1.9 3,501,748 - -2.2
Or see this region's nucleotide sequence