Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35195 and RR42_RS35200 are separated by 84 nucleotides RR42_RS35200 and RR42_RS35205 are separated by 334 nucleotides RR42_RS35205 and RR42_RS35210 are separated by 314 nucleotides
RR42_RS35195: RR42_RS35195 - 2,5-diketo-D-gluconic acid reductase, at 3,349,111 to 3,349,914
_RS35195
RR42_RS35200: RR42_RS35200 - epimerase, at 3,349,999 to 3,350,901
_RS35200
RR42_RS35205: RR42_RS35205 - translation initiation inhibitor, at 3,351,236 to 3,351,547
_RS35205
RR42_RS35210: RR42_RS35210 - TetR family transcriptional regulator, at 3,351,862 to 3,353,091
_RS35210
Position (kb)
3349
3350
3351 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3349.011 kb on + strand at 3349.011 kb on + strand at 3349.012 kb on - strand at 3349.032 kb on + strand at 3349.033 kb on - strand at 3349.062 kb on + strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.063 kb on - strand at 3349.071 kb on + strand at 3349.071 kb on + strand at 3349.072 kb on - strand at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.382 kb on + strand, within RR42_RS35195 at 3349.383 kb on - strand, within RR42_RS35195 at 3349.799 kb on + strand, within RR42_RS35195 at 3349.799 kb on + strand, within RR42_RS35195 at 3349.800 kb on - strand, within RR42_RS35195 at 3349.887 kb on + strand at 3350.114 kb on + strand, within RR42_RS35200 at 3350.220 kb on - strand, within RR42_RS35200 at 3350.246 kb on + strand, within RR42_RS35200 at 3350.246 kb on + strand, within RR42_RS35200 at 3350.247 kb on - strand, within RR42_RS35200 at 3350.285 kb on + strand, within RR42_RS35200 at 3350.286 kb on - strand, within RR42_RS35200 at 3350.613 kb on + strand, within RR42_RS35200 at 3350.663 kb on - strand, within RR42_RS35200 at 3350.826 kb on + strand at 3350.826 kb on + strand at 3350.827 kb on - strand at 3350.827 kb on - strand at 3350.827 kb on - strand at 3351.053 kb on + strand at 3351.053 kb on + strand at 3351.053 kb on + strand at 3351.053 kb on + strand at 3351.053 kb on + strand at 3351.054 kb on - strand at 3351.054 kb on - strand at 3351.054 kb on - strand at 3351.112 kb on + strand at 3351.113 kb on - strand at 3351.160 kb on - strand at 3351.220 kb on + strand at 3351.276 kb on + strand, within RR42_RS35205 at 3351.276 kb on + strand, within RR42_RS35205 at 3351.276 kb on + strand, within RR42_RS35205 at 3351.283 kb on + strand, within RR42_RS35205 at 3351.283 kb on + strand, within RR42_RS35205 at 3351.284 kb on - strand, within RR42_RS35205 at 3351.285 kb on + strand, within RR42_RS35205 at 3351.285 kb on + strand, within RR42_RS35205 at 3351.285 kb on + strand, within RR42_RS35205 at 3351.444 kb on + strand, within RR42_RS35205 at 3351.445 kb on - strand, within RR42_RS35205 at 3351.445 kb on - strand, within RR42_RS35205 at 3351.751 kb on - strand at 3351.819 kb on + strand at 3351.819 kb on + strand at 3351.820 kb on - strand at 3351.820 kb on - strand at 3351.820 kb on - strand at 3351.820 kb on - strand at 3351.820 kb on - strand at 3351.851 kb on + strand at 3351.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,349,011 + -0.2 3,349,011 + -0.9 3,349,012 - +1.1 3,349,032 + +1.4 3,349,033 - +0.2 3,349,062 + +0.3 3,349,063 - -1.1 3,349,063 - +0.2 3,349,063 - -1.0 3,349,063 - -0.4 3,349,063 - +0.7 3,349,063 - +1.0 3,349,071 + +0.4 3,349,071 + +0.9 3,349,072 - -0.1 3,349,382 + RR42_RS35195 0.34 -0.2 3,349,382 + RR42_RS35195 0.34 -1.5 3,349,382 + RR42_RS35195 0.34 -0.6 3,349,382 + RR42_RS35195 0.34 -1.4 3,349,382 + RR42_RS35195 0.34 -0.6 3,349,383 - RR42_RS35195 0.34 -0.8 3,349,799 + RR42_RS35195 0.86 -0.2 3,349,799 + RR42_RS35195 0.86 -0.1 3,349,800 - RR42_RS35195 0.86 +1.0 3,349,887 + -0.8 3,350,114 + RR42_RS35200 0.13 -0.7 3,350,220 - RR42_RS35200 0.24 +0.2 3,350,246 + RR42_RS35200 0.27 +1.1 3,350,246 + RR42_RS35200 0.27 +0.0 3,350,247 - RR42_RS35200 0.27 +1.2 3,350,285 + RR42_RS35200 0.32 -1.2 3,350,286 - RR42_RS35200 0.32 +1.6 3,350,613 + RR42_RS35200 0.68 -0.1 3,350,663 - RR42_RS35200 0.74 -0.6 3,350,826 + +0.1 3,350,826 + +0.8 3,350,827 - -1.1 3,350,827 - -1.2 3,350,827 - -0.7 3,351,053 + -0.6 3,351,053 + -0.1 3,351,053 + -1.2 3,351,053 + -0.6 3,351,053 + -0.3 3,351,054 - -1.1 3,351,054 - -2.7 3,351,054 - +1.0 3,351,112 + +1.4 3,351,113 - +0.7 3,351,160 - +0.5 3,351,220 + +1.4 3,351,276 + RR42_RS35205 0.13 -0.6 3,351,276 + RR42_RS35205 0.13 +0.4 3,351,276 + RR42_RS35205 0.13 +0.6 3,351,283 + RR42_RS35205 0.15 -0.3 3,351,283 + RR42_RS35205 0.15 -0.5 3,351,284 - RR42_RS35205 0.15 -1.2 3,351,285 + RR42_RS35205 0.16 +0.4 3,351,285 + RR42_RS35205 0.16 +1.4 3,351,285 + RR42_RS35205 0.16 -0.4 3,351,444 + RR42_RS35205 0.67 -0.2 3,351,445 - RR42_RS35205 0.67 +0.1 3,351,445 - RR42_RS35205 0.67 +1.4 3,351,751 - -1.1 3,351,819 + -0.2 3,351,819 + -0.6 3,351,820 - +2.0 3,351,820 - +0.1 3,351,820 - +0.4 3,351,820 - +0.1 3,351,820 - -0.3 3,351,851 + +0.9 3,351,852 - -0.5
Or see this region's nucleotide sequence