Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33930 and RR42_RS33935 are separated by 3 nucleotides RR42_RS33935 and RR42_RS33940 are separated by 29 nucleotides RR42_RS33940 and RR42_RS33945 are separated by 87 nucleotides RR42_RS33945 and RR42_RS33950 are separated by 94 nucleotides
RR42_RS33930: RR42_RS33930 - salicylate hydroxylase, at 3,039,525 to 3,040,781
_RS33930
RR42_RS33935: RR42_RS33935 - salicylate hydroxylase, at 3,040,785 to 3,041,258
_RS33935
RR42_RS33940: RR42_RS33940 - naphthalene 1,2-dioxygenase, at 3,041,288 to 3,041,599
_RS33940
RR42_RS33945: RR42_RS33945 - MFS transporter, at 3,041,687 to 3,043,027
_RS33945
RR42_RS33950: RR42_RS33950 - dihydroxy-acid dehydratase, at 3,043,122 to 3,044,855
_RS33950
Position (kb)
3041
3042
3043
3044 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3040.749 kb on + strand at 3040.750 kb on - strand at 3040.750 kb on - strand at 3040.755 kb on + strand at 3040.755 kb on + strand at 3040.756 kb on - strand at 3040.756 kb on - strand at 3040.803 kb on + strand at 3040.804 kb on - strand at 3040.804 kb on - strand at 3040.824 kb on + strand at 3040.824 kb on + strand at 3040.824 kb on + strand at 3040.825 kb on - strand at 3040.833 kb on + strand, within RR42_RS33935 at 3040.985 kb on + strand, within RR42_RS33935 at 3040.985 kb on + strand, within RR42_RS33935 at 3040.985 kb on + strand, within RR42_RS33935 at 3040.986 kb on - strand, within RR42_RS33935 at 3040.990 kb on - strand, within RR42_RS33935 at 3040.990 kb on - strand, within RR42_RS33935 at 3041.022 kb on + strand, within RR42_RS33935 at 3041.025 kb on + strand, within RR42_RS33935 at 3041.025 kb on + strand, within RR42_RS33935 at 3041.026 kb on - strand, within RR42_RS33935 at 3041.048 kb on - strand, within RR42_RS33935 at 3041.097 kb on + strand, within RR42_RS33935 at 3041.097 kb on + strand, within RR42_RS33935 at 3041.097 kb on + strand, within RR42_RS33935 at 3041.098 kb on - strand, within RR42_RS33935 at 3041.098 kb on - strand, within RR42_RS33935 at 3041.139 kb on + strand, within RR42_RS33935 at 3041.139 kb on + strand, within RR42_RS33935 at 3041.140 kb on - strand, within RR42_RS33935 at 3041.154 kb on + strand, within RR42_RS33935 at 3041.154 kb on + strand, within RR42_RS33935 at 3041.248 kb on - strand at 3041.267 kb on + strand at 3041.382 kb on - strand, within RR42_RS33940 at 3041.402 kb on + strand, within RR42_RS33940 at 3041.403 kb on - strand, within RR42_RS33940 at 3041.403 kb on - strand, within RR42_RS33940 at 3041.556 kb on - strand, within RR42_RS33940 at 3041.938 kb on - strand, within RR42_RS33945 at 3042.114 kb on + strand, within RR42_RS33945 at 3042.114 kb on + strand, within RR42_RS33945 at 3042.120 kb on - strand, within RR42_RS33945 at 3042.198 kb on + strand, within RR42_RS33945 at 3042.199 kb on - strand, within RR42_RS33945 at 3042.438 kb on - strand, within RR42_RS33945 at 3042.648 kb on + strand, within RR42_RS33945 at 3042.648 kb on + strand, within RR42_RS33945 at 3042.648 kb on + strand, within RR42_RS33945 at 3042.648 kb on + strand, within RR42_RS33945 at 3042.649 kb on - strand, within RR42_RS33945 at 3042.649 kb on - strand, within RR42_RS33945 at 3042.649 kb on - strand, within RR42_RS33945 at 3042.649 kb on - strand, within RR42_RS33945 at 3042.780 kb on + strand, within RR42_RS33945 at 3042.780 kb on + strand, within RR42_RS33945 at 3042.781 kb on - strand, within RR42_RS33945 at 3042.813 kb on + strand, within RR42_RS33945 at 3042.885 kb on + strand, within RR42_RS33945 at 3042.885 kb on + strand, within RR42_RS33945 at 3042.885 kb on + strand, within RR42_RS33945 at 3042.885 kb on + strand, within RR42_RS33945 at 3042.886 kb on - strand, within RR42_RS33945 at 3042.886 kb on - strand, within RR42_RS33945 at 3042.898 kb on - strand at 3042.943 kb on + strand at 3042.943 kb on + strand at 3042.943 kb on + strand at 3042.944 kb on - strand at 3042.944 kb on - strand at 3043.214 kb on - strand at 3043.214 kb on - strand at 3043.395 kb on + strand, within RR42_RS33950 at 3043.396 kb on - strand, within RR42_RS33950 at 3043.396 kb on - strand, within RR42_RS33950 at 3043.396 kb on - strand, within RR42_RS33950 at 3043.600 kb on + strand, within RR42_RS33950 at 3043.601 kb on - strand, within RR42_RS33950 at 3043.894 kb on + strand, within RR42_RS33950 at 3043.895 kb on - strand, within RR42_RS33950 at 3043.895 kb on - strand, within RR42_RS33950
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,040,749 + -0.9 3,040,750 - +0.2 3,040,750 - -0.9 3,040,755 + -0.6 3,040,755 + +2.9 3,040,756 - -2.7 3,040,756 - -1.1 3,040,803 + -0.7 3,040,804 - +1.5 3,040,804 - -1.4 3,040,824 + -0.0 3,040,824 + +0.6 3,040,824 + +0.2 3,040,825 - +2.3 3,040,833 + RR42_RS33935 0.10 -0.8 3,040,985 + RR42_RS33935 0.42 +1.3 3,040,985 + RR42_RS33935 0.42 +1.7 3,040,985 + RR42_RS33935 0.42 -1.4 3,040,986 - RR42_RS33935 0.42 -0.4 3,040,990 - RR42_RS33935 0.43 -0.9 3,040,990 - RR42_RS33935 0.43 -0.2 3,041,022 + RR42_RS33935 0.50 -0.6 3,041,025 + RR42_RS33935 0.51 -1.5 3,041,025 + RR42_RS33935 0.51 -0.3 3,041,026 - RR42_RS33935 0.51 +1.3 3,041,048 - RR42_RS33935 0.55 -0.1 3,041,097 + RR42_RS33935 0.66 +0.9 3,041,097 + RR42_RS33935 0.66 +0.4 3,041,097 + RR42_RS33935 0.66 +0.1 3,041,098 - RR42_RS33935 0.66 +0.1 3,041,098 - RR42_RS33935 0.66 +1.0 3,041,139 + RR42_RS33935 0.75 -0.4 3,041,139 + RR42_RS33935 0.75 -0.0 3,041,140 - RR42_RS33935 0.75 -0.5 3,041,154 + RR42_RS33935 0.78 +0.4 3,041,154 + RR42_RS33935 0.78 -0.8 3,041,248 - +0.1 3,041,267 + +0.2 3,041,382 - RR42_RS33940 0.30 -0.3 3,041,402 + RR42_RS33940 0.37 -0.9 3,041,403 - RR42_RS33940 0.37 -0.0 3,041,403 - RR42_RS33940 0.37 -0.1 3,041,556 - RR42_RS33940 0.86 -0.3 3,041,938 - RR42_RS33945 0.19 +0.1 3,042,114 + RR42_RS33945 0.32 +0.7 3,042,114 + RR42_RS33945 0.32 -1.4 3,042,120 - RR42_RS33945 0.32 +0.5 3,042,198 + RR42_RS33945 0.38 +0.5 3,042,199 - RR42_RS33945 0.38 +0.0 3,042,438 - RR42_RS33945 0.56 +0.7 3,042,648 + RR42_RS33945 0.72 +1.3 3,042,648 + RR42_RS33945 0.72 -0.1 3,042,648 + RR42_RS33945 0.72 -0.8 3,042,648 + RR42_RS33945 0.72 +0.3 3,042,649 - RR42_RS33945 0.72 +0.7 3,042,649 - RR42_RS33945 0.72 +0.5 3,042,649 - RR42_RS33945 0.72 +0.4 3,042,649 - RR42_RS33945 0.72 +0.6 3,042,780 + RR42_RS33945 0.82 +1.7 3,042,780 + RR42_RS33945 0.82 +0.1 3,042,781 - RR42_RS33945 0.82 +1.0 3,042,813 + RR42_RS33945 0.84 -0.5 3,042,885 + RR42_RS33945 0.89 -2.4 3,042,885 + RR42_RS33945 0.89 +0.3 3,042,885 + RR42_RS33945 0.89 -1.0 3,042,885 + RR42_RS33945 0.89 -0.6 3,042,886 - RR42_RS33945 0.89 +1.3 3,042,886 - RR42_RS33945 0.89 +0.5 3,042,898 - +0.6 3,042,943 + -1.3 3,042,943 + +0.7 3,042,943 + -0.0 3,042,944 - -1.4 3,042,944 - +0.4 3,043,214 - -0.6 3,043,214 - +0.0 3,043,395 + RR42_RS33950 0.16 +0.4 3,043,396 - RR42_RS33950 0.16 +0.1 3,043,396 - RR42_RS33950 0.16 -0.5 3,043,396 - RR42_RS33950 0.16 -1.0 3,043,600 + RR42_RS33950 0.28 -0.7 3,043,601 - RR42_RS33950 0.28 +3.3 3,043,894 + RR42_RS33950 0.45 +0.6 3,043,895 - RR42_RS33950 0.45 +0.7 3,043,895 - RR42_RS33950 0.45 -1.1
Or see this region's nucleotide sequence