Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30895 and RR42_RS30900 overlap by 4 nucleotides RR42_RS30900 and RR42_RS30905 are separated by 48 nucleotides RR42_RS30905 and RR42_RS30910 overlap by 22 nucleotides RR42_RS30910 and RR42_RS30915 overlap by 13 nucleotides
RR42_RS30895: RR42_RS30895 - 4-amino-4-deoxy-L-arabinose transferase, at 2,312,928 to 2,314,700
_RS30895
RR42_RS30900: RR42_RS30900 - 4-amino-4-deoxy-L-arabinose transferase, at 2,314,697 to 2,315,068
_RS30900
RR42_RS30905: RR42_RS30905 - aminotransferase DegT, at 2,315,117 to 2,316,280
_RS30905
RR42_RS30910: RR42_RS30910 - UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase, at 2,316,259 to 2,317,251
_RS30910
RR42_RS30915: RR42_RS30915 - formyltransferase, at 2,317,239 to 2,318,198
_RS30915
Position (kb) 2315
2316
2317 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 2314.708 kb on + strand at 2314.708 kb on + strand at 2315.516 kb on + strand, within RR42_RS30905 at 2315.912 kb on + strand, within RR42_RS30905 at 2316.122 kb on + strand, within RR42_RS30905 at 2316.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 2,314,708 + -3.1 2,314,708 + -2.3 2,315,516 + RR42_RS30905 0.34 +0.8 2,315,912 + RR42_RS30905 0.68 -0.6 2,316,122 + RR42_RS30905 0.86 +0.2 2,316,250 + -1.6
Or see this region's nucleotide sequence