Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS27840

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS27835 and RR42_RS27840 are separated by 37 nucleotidesRR42_RS27840 and RR42_RS27845 are separated by 65 nucleotidesRR42_RS27845 and RR42_RS27850 are separated by 63 nucleotides RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168 _RS27835 RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054 _RS27840 RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509 _RS27845 RR42_RS27850: RR42_RS27850 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 1,611,573 to 1,612,376 _RS27850 Position (kb) 1610 1611 1612Strain fitness (log2 ratio) -1 0 1 2 3at 1609.695 kb on + strand, within RR42_RS27835at 1609.695 kb on + strand, within RR42_RS27835at 1609.696 kb on - strand, within RR42_RS27835at 1609.696 kb on - strand, within RR42_RS27835at 1609.923 kb on + strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.924 kb on - strand, within RR42_RS27835at 1609.938 kb on + strand, within RR42_RS27835at 1609.939 kb on - strand, within RR42_RS27835at 1610.147 kb on + strandat 1610.148 kb on - strandat 1610.216 kb on + strandat 1610.216 kb on + strandat 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.431 kb on + strand, within RR42_RS27840at 1610.474 kb on - strand, within RR42_RS27840at 1610.603 kb on - strand, within RR42_RS27840at 1610.897 kb on - strand, within RR42_RS27840at 1610.972 kb on - strandat 1610.973 kb on - strandat 1611.142 kb on - strandat 1611.298 kb on - strand, within RR42_RS27845at 1611.298 kb on - strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.406 kb on + strand, within RR42_RS27845at 1611.407 kb on - strand, within RR42_RS27845at 1611.409 kb on - strand, within RR42_RS27845at 1611.409 kb on - strand, within RR42_RS27845at 1611.468 kb on + strand, within RR42_RS27845at 1611.690 kb on + strand, within RR42_RS27850at 1611.811 kb on - strand, within RR42_RS27850at 1611.954 kb on + strand, within RR42_RS27850at 1611.954 kb on + strand, within RR42_RS27850at 1611.955 kb on - strand, within RR42_RS27850at 1611.969 kb on + strand, within RR42_RS27850at 1611.969 kb on + strand, within RR42_RS27850

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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1,609,695 + RR42_RS27835 0.68 -0.6
1,609,695 + RR42_RS27835 0.68 -0.8
1,609,696 - RR42_RS27835 0.68 +0.9
1,609,696 - RR42_RS27835 0.68 +0.5
1,609,923 + RR42_RS27835 0.83 -1.4
1,609,924 - RR42_RS27835 0.83 +0.2
1,609,924 - RR42_RS27835 0.83 -0.3
1,609,924 - RR42_RS27835 0.83 +0.7
1,609,938 + RR42_RS27835 0.84 +1.4
1,609,939 - RR42_RS27835 0.84 +0.1
1,610,147 + -1.2
1,610,148 - -1.3
1,610,216 + -0.3
1,610,216 + +0.1
1,610,431 + RR42_RS27840 0.27 +0.3
1,610,431 + RR42_RS27840 0.27 -1.1
1,610,431 + RR42_RS27840 0.27 -0.5
1,610,431 + RR42_RS27840 0.27 +1.0
1,610,474 - RR42_RS27840 0.32 +1.0
1,610,603 - RR42_RS27840 0.47 -0.5
1,610,897 - RR42_RS27840 0.81 -0.7
1,610,972 - +0.5
1,610,973 - +0.4
1,611,142 - +3.7
1,611,298 - RR42_RS27845 0.46 +0.1
1,611,298 - RR42_RS27845 0.46 -1.3
1,611,406 + RR42_RS27845 0.73 -1.7
1,611,406 + RR42_RS27845 0.73 -1.2
1,611,406 + RR42_RS27845 0.73 +0.5
1,611,407 - RR42_RS27845 0.74 +0.6
1,611,409 - RR42_RS27845 0.74 -0.5
1,611,409 - RR42_RS27845 0.74 -0.2
1,611,468 + RR42_RS27845 0.89 +0.4
1,611,690 + RR42_RS27850 0.15 -0.0
1,611,811 - RR42_RS27850 0.30 -0.5
1,611,954 + RR42_RS27850 0.47 -1.1
1,611,954 + RR42_RS27850 0.47 -0.2
1,611,955 - RR42_RS27850 0.48 -0.4
1,611,969 + RR42_RS27850 0.49 -1.2
1,611,969 + RR42_RS27850 0.49 -0.0

Or see this region's nucleotide sequence