Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26280 and RR42_RS26285 are separated by 100 nucleotides RR42_RS26285 and RR42_RS26290 are separated by 276 nucleotides RR42_RS26290 and RR42_RS26295 are separated by 133 nucleotides RR42_RS26295 and RR42_RS26300 are separated by 119 nucleotides
RR42_RS26280: RR42_RS26280 - poly(3-hydroxyalkanoate) depolymerase, at 1,255,700 to 1,256,770
_RS26280
RR42_RS26285: RR42_RS26285 - ureidoglycolate hydrolase, at 1,256,871 to 1,257,389
_RS26285
RR42_RS26290: RR42_RS26290 - MFS transporter permease, at 1,257,666 to 1,258,985
_RS26290
RR42_RS26295: RR42_RS26295 - GntR family transcriptional regulator, at 1,259,119 to 1,259,847
_RS26295
RR42_RS26300: RR42_RS26300 - chemotaxis protein, at 1,259,967 to 1,261,163
_RS26300
Position (kb)
1257
1258
1259 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1256.674 kb on + strand at 1256.674 kb on + strand at 1256.674 kb on + strand at 1256.675 kb on - strand at 1256.675 kb on - strand at 1256.675 kb on - strand at 1256.675 kb on - strand at 1256.687 kb on + strand at 1256.768 kb on + strand at 1256.768 kb on + strand at 1256.768 kb on + strand at 1256.768 kb on + strand at 1256.769 kb on - strand at 1256.769 kb on - strand at 1256.769 kb on - strand at 1256.769 kb on - strand at 1256.887 kb on + strand at 1256.887 kb on + strand at 1256.888 kb on - strand at 1257.004 kb on + strand, within RR42_RS26285 at 1257.004 kb on + strand, within RR42_RS26285 at 1257.004 kb on + strand, within RR42_RS26285 at 1257.005 kb on - strand, within RR42_RS26285 at 1257.172 kb on + strand, within RR42_RS26285 at 1257.331 kb on - strand, within RR42_RS26285 at 1257.536 kb on + strand at 1257.536 kb on + strand at 1257.536 kb on + strand at 1257.736 kb on - strand at 1257.736 kb on - strand at 1257.780 kb on + strand at 1257.780 kb on + strand at 1257.780 kb on + strand at 1257.780 kb on + strand at 1257.781 kb on - strand at 1257.781 kb on - strand at 1257.781 kb on - strand at 1257.781 kb on - strand at 1257.830 kb on + strand, within RR42_RS26290 at 1257.830 kb on + strand, within RR42_RS26290 at 1257.830 kb on + strand, within RR42_RS26290 at 1257.831 kb on - strand, within RR42_RS26290 at 1257.831 kb on - strand, within RR42_RS26290 at 1257.831 kb on - strand, within RR42_RS26290 at 1257.897 kb on + strand, within RR42_RS26290 at 1257.897 kb on + strand, within RR42_RS26290 at 1257.898 kb on - strand, within RR42_RS26290 at 1258.029 kb on + strand, within RR42_RS26290 at 1258.029 kb on + strand, within RR42_RS26290 at 1258.030 kb on - strand, within RR42_RS26290 at 1258.030 kb on - strand, within RR42_RS26290 at 1258.030 kb on - strand, within RR42_RS26290 at 1258.030 kb on - strand, within RR42_RS26290 at 1258.030 kb on - strand, within RR42_RS26290 at 1258.089 kb on + strand, within RR42_RS26290 at 1258.089 kb on + strand, within RR42_RS26290 at 1258.098 kb on + strand, within RR42_RS26290 at 1258.099 kb on - strand, within RR42_RS26290 at 1258.296 kb on + strand, within RR42_RS26290 at 1258.477 kb on - strand, within RR42_RS26290 at 1258.539 kb on - strand, within RR42_RS26290 at 1258.860 kb on + strand at 1258.861 kb on - strand at 1258.861 kb on - strand at 1258.861 kb on - strand at 1258.861 kb on - strand at 1258.861 kb on - strand at 1258.861 kb on - strand at 1258.953 kb on + strand at 1258.953 kb on + strand at 1258.953 kb on + strand at 1258.954 kb on - strand at 1258.954 kb on - strand at 1258.955 kb on + strand at 1258.955 kb on + strand at 1258.956 kb on - strand at 1258.956 kb on - strand at 1258.983 kb on + strand at 1258.997 kb on - strand at 1259.041 kb on + strand at 1259.042 kb on - strand at 1259.131 kb on - strand at 1259.131 kb on - strand at 1259.189 kb on - strand at 1259.323 kb on + strand, within RR42_RS26295 at 1259.323 kb on + strand, within RR42_RS26295 at 1259.324 kb on - strand, within RR42_RS26295 at 1259.324 kb on - strand, within RR42_RS26295 at 1259.324 kb on - strand, within RR42_RS26295 at 1259.394 kb on + strand, within RR42_RS26295 at 1259.394 kb on + strand, within RR42_RS26295 at 1259.398 kb on + strand, within RR42_RS26295 at 1259.399 kb on - strand, within RR42_RS26295 at 1259.399 kb on - strand, within RR42_RS26295 at 1259.399 kb on - strand, within RR42_RS26295 at 1259.602 kb on - strand, within RR42_RS26295 at 1259.654 kb on - strand, within RR42_RS26295 at 1259.654 kb on - strand, within RR42_RS26295 at 1259.654 kb on - strand, within RR42_RS26295 at 1259.680 kb on + strand, within RR42_RS26295 at 1259.681 kb on - strand, within RR42_RS26295 at 1259.872 kb on - strand at 1259.893 kb on + strand at 1259.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,256,674 + +0.4 1,256,674 + +1.0 1,256,674 + -0.3 1,256,675 - -0.5 1,256,675 - -0.6 1,256,675 - -0.8 1,256,675 - +0.5 1,256,687 + -0.9 1,256,768 + -1.0 1,256,768 + -0.3 1,256,768 + -0.4 1,256,768 + -1.2 1,256,769 - -1.8 1,256,769 - -0.9 1,256,769 - +0.9 1,256,769 - -0.1 1,256,887 + -0.2 1,256,887 + +0.2 1,256,888 - +0.2 1,257,004 + RR42_RS26285 0.26 -0.5 1,257,004 + RR42_RS26285 0.26 -2.1 1,257,004 + RR42_RS26285 0.26 -0.6 1,257,005 - RR42_RS26285 0.26 -1.5 1,257,172 + RR42_RS26285 0.58 -0.5 1,257,331 - RR42_RS26285 0.89 -0.5 1,257,536 + +2.8 1,257,536 + -1.3 1,257,536 + -0.0 1,257,736 - -2.3 1,257,736 - -0.6 1,257,780 + +0.4 1,257,780 + -0.3 1,257,780 + -0.6 1,257,780 + +0.1 1,257,781 - +0.1 1,257,781 - +1.1 1,257,781 - -0.4 1,257,781 - -0.1 1,257,830 + RR42_RS26290 0.12 +0.2 1,257,830 + RR42_RS26290 0.12 +0.9 1,257,830 + RR42_RS26290 0.12 -0.3 1,257,831 - RR42_RS26290 0.12 +0.5 1,257,831 - RR42_RS26290 0.12 +0.8 1,257,831 - RR42_RS26290 0.12 +1.8 1,257,897 + RR42_RS26290 0.17 -0.3 1,257,897 + RR42_RS26290 0.17 +0.7 1,257,898 - RR42_RS26290 0.18 -0.6 1,258,029 + RR42_RS26290 0.28 +0.1 1,258,029 + RR42_RS26290 0.28 +1.3 1,258,030 - RR42_RS26290 0.28 +0.5 1,258,030 - RR42_RS26290 0.28 +0.3 1,258,030 - RR42_RS26290 0.28 -0.1 1,258,030 - RR42_RS26290 0.28 -0.2 1,258,030 - RR42_RS26290 0.28 -0.5 1,258,089 + RR42_RS26290 0.32 -2.2 1,258,089 + RR42_RS26290 0.32 -1.6 1,258,098 + RR42_RS26290 0.33 -0.5 1,258,099 - RR42_RS26290 0.33 -1.0 1,258,296 + RR42_RS26290 0.48 +0.0 1,258,477 - RR42_RS26290 0.61 -0.6 1,258,539 - RR42_RS26290 0.66 -0.3 1,258,860 + +0.1 1,258,861 - +1.3 1,258,861 - +0.4 1,258,861 - -1.6 1,258,861 - -0.8 1,258,861 - +0.3 1,258,861 - -0.8 1,258,953 + +0.1 1,258,953 + -0.4 1,258,953 + -1.1 1,258,954 - -0.8 1,258,954 - +1.8 1,258,955 + -0.5 1,258,955 + -0.3 1,258,956 - +0.0 1,258,956 - +1.3 1,258,983 + -0.5 1,258,997 - +1.2 1,259,041 + +0.5 1,259,042 - -0.1 1,259,131 - +1.1 1,259,131 - +0.1 1,259,189 - +0.4 1,259,323 + RR42_RS26295 0.28 -0.6 1,259,323 + RR42_RS26295 0.28 -0.2 1,259,324 - RR42_RS26295 0.28 -0.3 1,259,324 - RR42_RS26295 0.28 -0.6 1,259,324 - RR42_RS26295 0.28 +0.4 1,259,394 + RR42_RS26295 0.38 -1.8 1,259,394 + RR42_RS26295 0.38 +0.2 1,259,398 + RR42_RS26295 0.38 -0.7 1,259,399 - RR42_RS26295 0.38 -1.1 1,259,399 - RR42_RS26295 0.38 -0.2 1,259,399 - RR42_RS26295 0.38 +0.5 1,259,602 - RR42_RS26295 0.66 -0.6 1,259,654 - RR42_RS26295 0.73 +0.7 1,259,654 - RR42_RS26295 0.73 -0.7 1,259,654 - RR42_RS26295 0.73 +0.4 1,259,680 + RR42_RS26295 0.77 +0.8 1,259,681 - RR42_RS26295 0.77 +0.4 1,259,872 - -0.6 1,259,893 + -1.7 1,259,948 - -2.2
Or see this region's nucleotide sequence