Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26120 and RR42_RS26125 are separated by 20 nucleotides RR42_RS26125 and RR42_RS26130 are separated by 225 nucleotides
RR42_RS26120: RR42_RS26120 - dihydrolipoamide acetyltransferase, at 1,212,216 to 1,213,838
_RS26120
RR42_RS26125: RR42_RS26125 - pyruvate dehydrogenase, at 1,213,859 to 1,216,552
_RS26125
RR42_RS26130: RR42_RS26130 - C4-dicarboxylate ABC transporter, at 1,216,778 to 1,218,088
_RS26130
Position (kb)
1213
1214
1215
1216
1217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1213.129 kb on + strand, within RR42_RS26120 at 1213.410 kb on + strand, within RR42_RS26120 at 1213.845 kb on + strand at 1213.845 kb on + strand at 1213.983 kb on + strand at 1214.023 kb on - strand at 1214.119 kb on - strand at 1214.119 kb on - strand at 1214.251 kb on - strand, within RR42_RS26125 at 1214.400 kb on + strand, within RR42_RS26125 at 1214.469 kb on + strand, within RR42_RS26125 at 1214.469 kb on + strand, within RR42_RS26125 at 1214.469 kb on + strand, within RR42_RS26125 at 1214.470 kb on - strand, within RR42_RS26125 at 1214.470 kb on - strand, within RR42_RS26125 at 1214.493 kb on + strand, within RR42_RS26125 at 1214.494 kb on - strand, within RR42_RS26125 at 1214.494 kb on - strand, within RR42_RS26125 at 1214.499 kb on + strand, within RR42_RS26125 at 1214.499 kb on + strand, within RR42_RS26125 at 1214.499 kb on + strand, within RR42_RS26125 at 1214.520 kb on + strand, within RR42_RS26125 at 1214.520 kb on + strand, within RR42_RS26125 at 1214.521 kb on - strand, within RR42_RS26125 at 1214.521 kb on - strand, within RR42_RS26125 at 1214.758 kb on - strand, within RR42_RS26125 at 1214.760 kb on + strand, within RR42_RS26125 at 1214.760 kb on + strand, within RR42_RS26125 at 1214.761 kb on - strand, within RR42_RS26125 at 1214.761 kb on - strand, within RR42_RS26125 at 1214.761 kb on - strand, within RR42_RS26125 at 1214.766 kb on + strand, within RR42_RS26125 at 1214.767 kb on - strand, within RR42_RS26125 at 1214.767 kb on - strand, within RR42_RS26125 at 1214.887 kb on - strand, within RR42_RS26125 at 1214.889 kb on + strand, within RR42_RS26125 at 1214.889 kb on + strand, within RR42_RS26125 at 1214.919 kb on + strand, within RR42_RS26125 at 1214.919 kb on + strand, within RR42_RS26125 at 1214.920 kb on - strand, within RR42_RS26125 at 1214.940 kb on + strand, within RR42_RS26125 at 1214.941 kb on - strand, within RR42_RS26125 at 1214.941 kb on - strand, within RR42_RS26125 at 1214.941 kb on - strand, within RR42_RS26125 at 1215.204 kb on + strand, within RR42_RS26125 at 1215.204 kb on + strand, within RR42_RS26125 at 1215.373 kb on - strand, within RR42_RS26125 at 1215.373 kb on - strand, within RR42_RS26125 at 1215.435 kb on + strand, within RR42_RS26125 at 1215.532 kb on - strand, within RR42_RS26125 at 1215.924 kb on + strand, within RR42_RS26125 at 1215.924 kb on + strand, within RR42_RS26125 at 1215.946 kb on - strand, within RR42_RS26125 at 1216.110 kb on + strand, within RR42_RS26125 at 1216.110 kb on + strand, within RR42_RS26125 at 1216.110 kb on + strand, within RR42_RS26125 at 1216.111 kb on - strand, within RR42_RS26125 at 1216.111 kb on - strand, within RR42_RS26125 at 1216.111 kb on - strand, within RR42_RS26125 at 1216.141 kb on - strand, within RR42_RS26125 at 1216.203 kb on + strand, within RR42_RS26125 at 1216.404 kb on + strand at 1216.404 kb on + strand at 1216.405 kb on - strand at 1216.405 kb on - strand at 1216.405 kb on - strand at 1216.405 kb on - strand at 1216.439 kb on - strand at 1216.453 kb on + strand at 1216.552 kb on + strand at 1216.552 kb on + strand at 1216.552 kb on + strand at 1216.552 kb on + strand at 1216.553 kb on - strand at 1216.553 kb on - strand at 1217.019 kb on - strand, within RR42_RS26130 at 1217.119 kb on - strand, within RR42_RS26130 at 1217.154 kb on + strand, within RR42_RS26130 at 1217.155 kb on - strand, within RR42_RS26130 at 1217.425 kb on + strand, within RR42_RS26130 at 1217.426 kb on - strand, within RR42_RS26130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,213,129 + RR42_RS26120 0.56 -2.3 1,213,410 + RR42_RS26120 0.74 -1.5 1,213,845 + +1.3 1,213,845 + -2.4 1,213,983 + -0.9 1,214,023 - -1.3 1,214,119 - +0.7 1,214,119 - -0.5 1,214,251 - RR42_RS26125 0.15 -0.4 1,214,400 + RR42_RS26125 0.20 +1.0 1,214,469 + RR42_RS26125 0.23 -1.6 1,214,469 + RR42_RS26125 0.23 +0.9 1,214,469 + RR42_RS26125 0.23 -1.1 1,214,470 - RR42_RS26125 0.23 -1.3 1,214,470 - RR42_RS26125 0.23 +0.3 1,214,493 + RR42_RS26125 0.24 -1.4 1,214,494 - RR42_RS26125 0.24 -0.2 1,214,494 - RR42_RS26125 0.24 -1.4 1,214,499 + RR42_RS26125 0.24 +1.7 1,214,499 + RR42_RS26125 0.24 -2.6 1,214,499 + RR42_RS26125 0.24 -2.9 1,214,520 + RR42_RS26125 0.25 -1.6 1,214,520 + RR42_RS26125 0.25 -0.5 1,214,521 - RR42_RS26125 0.25 -1.0 1,214,521 - RR42_RS26125 0.25 -1.2 1,214,758 - RR42_RS26125 0.33 +2.0 1,214,760 + RR42_RS26125 0.33 -0.9 1,214,760 + RR42_RS26125 0.33 +1.3 1,214,761 - RR42_RS26125 0.33 -0.1 1,214,761 - RR42_RS26125 0.33 +0.5 1,214,761 - RR42_RS26125 0.33 -0.1 1,214,766 + RR42_RS26125 0.34 -1.3 1,214,767 - RR42_RS26125 0.34 -0.5 1,214,767 - RR42_RS26125 0.34 -0.7 1,214,887 - RR42_RS26125 0.38 +1.6 1,214,889 + RR42_RS26125 0.38 +0.4 1,214,889 + RR42_RS26125 0.38 -1.1 1,214,919 + RR42_RS26125 0.39 -0.1 1,214,919 + RR42_RS26125 0.39 -1.1 1,214,920 - RR42_RS26125 0.39 +3.3 1,214,940 + RR42_RS26125 0.40 -0.6 1,214,941 - RR42_RS26125 0.40 -0.4 1,214,941 - RR42_RS26125 0.40 -1.3 1,214,941 - RR42_RS26125 0.40 -2.6 1,215,204 + RR42_RS26125 0.50 +0.8 1,215,204 + RR42_RS26125 0.50 +0.9 1,215,373 - RR42_RS26125 0.56 -3.3 1,215,373 - RR42_RS26125 0.56 +0.5 1,215,435 + RR42_RS26125 0.59 -2.7 1,215,532 - RR42_RS26125 0.62 -1.8 1,215,924 + RR42_RS26125 0.77 -0.4 1,215,924 + RR42_RS26125 0.77 -0.2 1,215,946 - RR42_RS26125 0.77 -0.7 1,216,110 + RR42_RS26125 0.84 +1.5 1,216,110 + RR42_RS26125 0.84 +0.0 1,216,110 + RR42_RS26125 0.84 -0.4 1,216,111 - RR42_RS26125 0.84 +0.2 1,216,111 - RR42_RS26125 0.84 -0.5 1,216,111 - RR42_RS26125 0.84 -1.8 1,216,141 - RR42_RS26125 0.85 +0.1 1,216,203 + RR42_RS26125 0.87 -2.2 1,216,404 + +0.4 1,216,404 + +0.3 1,216,405 - +2.3 1,216,405 - -0.4 1,216,405 - +1.3 1,216,405 - -1.0 1,216,439 - -1.1 1,216,453 + +1.7 1,216,552 + -0.1 1,216,552 + -0.3 1,216,552 + +1.1 1,216,552 + +0.5 1,216,553 - -0.2 1,216,553 - +1.8 1,217,019 - RR42_RS26130 0.18 +0.4 1,217,119 - RR42_RS26130 0.26 +1.3 1,217,154 + RR42_RS26130 0.29 +2.7 1,217,155 - RR42_RS26130 0.29 -1.0 1,217,425 + RR42_RS26130 0.49 -0.5 1,217,426 - RR42_RS26130 0.49 +0.8
Or see this region's nucleotide sequence