Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS26125

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS26120 and RR42_RS26125 are separated by 20 nucleotidesRR42_RS26125 and RR42_RS26130 are separated by 225 nucleotides RR42_RS26120: RR42_RS26120 - dihydrolipoamide acetyltransferase, at 1,212,216 to 1,213,838 _RS26120 RR42_RS26125: RR42_RS26125 - pyruvate dehydrogenase, at 1,213,859 to 1,216,552 _RS26125 RR42_RS26130: RR42_RS26130 - C4-dicarboxylate ABC transporter, at 1,216,778 to 1,218,088 _RS26130 Position (kb) 1213 1214 1215 1216 1217Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1213.129 kb on + strand, within RR42_RS26120at 1213.410 kb on + strand, within RR42_RS26120at 1213.845 kb on + strandat 1213.845 kb on + strandat 1213.983 kb on + strandat 1214.023 kb on - strandat 1214.119 kb on - strandat 1214.119 kb on - strandat 1214.251 kb on - strand, within RR42_RS26125at 1214.400 kb on + strand, within RR42_RS26125at 1214.469 kb on + strand, within RR42_RS26125at 1214.469 kb on + strand, within RR42_RS26125at 1214.469 kb on + strand, within RR42_RS26125at 1214.470 kb on - strand, within RR42_RS26125at 1214.470 kb on - strand, within RR42_RS26125at 1214.493 kb on + strand, within RR42_RS26125at 1214.494 kb on - strand, within RR42_RS26125at 1214.494 kb on - strand, within RR42_RS26125at 1214.499 kb on + strand, within RR42_RS26125at 1214.499 kb on + strand, within RR42_RS26125at 1214.499 kb on + strand, within RR42_RS26125at 1214.520 kb on + strand, within RR42_RS26125at 1214.520 kb on + strand, within RR42_RS26125at 1214.521 kb on - strand, within RR42_RS26125at 1214.521 kb on - strand, within RR42_RS26125at 1214.758 kb on - strand, within RR42_RS26125at 1214.760 kb on + strand, within RR42_RS26125at 1214.760 kb on + strand, within RR42_RS26125at 1214.761 kb on - strand, within RR42_RS26125at 1214.761 kb on - strand, within RR42_RS26125at 1214.761 kb on - strand, within RR42_RS26125at 1214.766 kb on + strand, within RR42_RS26125at 1214.767 kb on - strand, within RR42_RS26125at 1214.767 kb on - strand, within RR42_RS26125at 1214.887 kb on - strand, within RR42_RS26125at 1214.889 kb on + strand, within RR42_RS26125at 1214.889 kb on + strand, within RR42_RS26125at 1214.919 kb on + strand, within RR42_RS26125at 1214.919 kb on + strand, within RR42_RS26125at 1214.920 kb on - strand, within RR42_RS26125at 1214.940 kb on + strand, within RR42_RS26125at 1214.941 kb on - strand, within RR42_RS26125at 1214.941 kb on - strand, within RR42_RS26125at 1214.941 kb on - strand, within RR42_RS26125at 1215.204 kb on + strand, within RR42_RS26125at 1215.204 kb on + strand, within RR42_RS26125at 1215.373 kb on - strand, within RR42_RS26125at 1215.373 kb on - strand, within RR42_RS26125at 1215.435 kb on + strand, within RR42_RS26125at 1215.532 kb on - strand, within RR42_RS26125at 1215.924 kb on + strand, within RR42_RS26125at 1215.924 kb on + strand, within RR42_RS26125at 1215.946 kb on - strand, within RR42_RS26125at 1216.110 kb on + strand, within RR42_RS26125at 1216.110 kb on + strand, within RR42_RS26125at 1216.110 kb on + strand, within RR42_RS26125at 1216.111 kb on - strand, within RR42_RS26125at 1216.111 kb on - strand, within RR42_RS26125at 1216.111 kb on - strand, within RR42_RS26125at 1216.141 kb on - strand, within RR42_RS26125at 1216.203 kb on + strand, within RR42_RS26125at 1216.404 kb on + strandat 1216.404 kb on + strandat 1216.405 kb on - strandat 1216.405 kb on - strandat 1216.405 kb on - strandat 1216.405 kb on - strandat 1216.439 kb on - strandat 1216.453 kb on + strandat 1216.552 kb on + strandat 1216.552 kb on + strandat 1216.552 kb on + strandat 1216.552 kb on + strandat 1216.553 kb on - strandat 1216.553 kb on - strandat 1217.019 kb on - strand, within RR42_RS26130at 1217.119 kb on - strand, within RR42_RS26130at 1217.154 kb on + strand, within RR42_RS26130at 1217.155 kb on - strand, within RR42_RS26130at 1217.425 kb on + strand, within RR42_RS26130at 1217.426 kb on - strand, within RR42_RS26130

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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1,213,129 + RR42_RS26120 0.56 -2.3
1,213,410 + RR42_RS26120 0.74 -1.5
1,213,845 + +1.3
1,213,845 + -2.4
1,213,983 + -0.9
1,214,023 - -1.3
1,214,119 - +0.7
1,214,119 - -0.5
1,214,251 - RR42_RS26125 0.15 -0.4
1,214,400 + RR42_RS26125 0.20 +1.0
1,214,469 + RR42_RS26125 0.23 -1.6
1,214,469 + RR42_RS26125 0.23 +0.9
1,214,469 + RR42_RS26125 0.23 -1.1
1,214,470 - RR42_RS26125 0.23 -1.3
1,214,470 - RR42_RS26125 0.23 +0.3
1,214,493 + RR42_RS26125 0.24 -1.4
1,214,494 - RR42_RS26125 0.24 -0.2
1,214,494 - RR42_RS26125 0.24 -1.4
1,214,499 + RR42_RS26125 0.24 +1.7
1,214,499 + RR42_RS26125 0.24 -2.6
1,214,499 + RR42_RS26125 0.24 -2.9
1,214,520 + RR42_RS26125 0.25 -1.6
1,214,520 + RR42_RS26125 0.25 -0.5
1,214,521 - RR42_RS26125 0.25 -1.0
1,214,521 - RR42_RS26125 0.25 -1.2
1,214,758 - RR42_RS26125 0.33 +2.0
1,214,760 + RR42_RS26125 0.33 -0.9
1,214,760 + RR42_RS26125 0.33 +1.3
1,214,761 - RR42_RS26125 0.33 -0.1
1,214,761 - RR42_RS26125 0.33 +0.5
1,214,761 - RR42_RS26125 0.33 -0.1
1,214,766 + RR42_RS26125 0.34 -1.3
1,214,767 - RR42_RS26125 0.34 -0.5
1,214,767 - RR42_RS26125 0.34 -0.7
1,214,887 - RR42_RS26125 0.38 +1.6
1,214,889 + RR42_RS26125 0.38 +0.4
1,214,889 + RR42_RS26125 0.38 -1.1
1,214,919 + RR42_RS26125 0.39 -0.1
1,214,919 + RR42_RS26125 0.39 -1.1
1,214,920 - RR42_RS26125 0.39 +3.3
1,214,940 + RR42_RS26125 0.40 -0.6
1,214,941 - RR42_RS26125 0.40 -0.4
1,214,941 - RR42_RS26125 0.40 -1.3
1,214,941 - RR42_RS26125 0.40 -2.6
1,215,204 + RR42_RS26125 0.50 +0.8
1,215,204 + RR42_RS26125 0.50 +0.9
1,215,373 - RR42_RS26125 0.56 -3.3
1,215,373 - RR42_RS26125 0.56 +0.5
1,215,435 + RR42_RS26125 0.59 -2.7
1,215,532 - RR42_RS26125 0.62 -1.8
1,215,924 + RR42_RS26125 0.77 -0.4
1,215,924 + RR42_RS26125 0.77 -0.2
1,215,946 - RR42_RS26125 0.77 -0.7
1,216,110 + RR42_RS26125 0.84 +1.5
1,216,110 + RR42_RS26125 0.84 +0.0
1,216,110 + RR42_RS26125 0.84 -0.4
1,216,111 - RR42_RS26125 0.84 +0.2
1,216,111 - RR42_RS26125 0.84 -0.5
1,216,111 - RR42_RS26125 0.84 -1.8
1,216,141 - RR42_RS26125 0.85 +0.1
1,216,203 + RR42_RS26125 0.87 -2.2
1,216,404 + +0.4
1,216,404 + +0.3
1,216,405 - +2.3
1,216,405 - -0.4
1,216,405 - +1.3
1,216,405 - -1.0
1,216,439 - -1.1
1,216,453 + +1.7
1,216,552 + -0.1
1,216,552 + -0.3
1,216,552 + +1.1
1,216,552 + +0.5
1,216,553 - -0.2
1,216,553 - +1.8
1,217,019 - RR42_RS26130 0.18 +0.4
1,217,119 - RR42_RS26130 0.26 +1.3
1,217,154 + RR42_RS26130 0.29 +2.7
1,217,155 - RR42_RS26130 0.29 -1.0
1,217,425 + RR42_RS26130 0.49 -0.5
1,217,426 - RR42_RS26130 0.49 +0.8

Or see this region's nucleotide sequence