Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24245 and RR42_RS24250 are separated by 255 nucleotides RR42_RS24250 and RR42_RS24255 overlap by 4 nucleotides
RR42_RS24245: RR42_RS24245 - porin, at 765,255 to 766,322
_RS24245
RR42_RS24250: RR42_RS24250 - (2Fe-2S)-binding protein, at 766,578 to 767,666
_RS24250
RR42_RS24255: RR42_RS24255 - hypothetical protein, at 767,663 to 768,619
_RS24255
Position (kb) 766
767
768 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 765.586 kb on - strand, within RR42_RS24245 at 765.595 kb on + strand, within RR42_RS24245 at 765.595 kb on + strand, within RR42_RS24245 at 765.595 kb on + strand, within RR42_RS24245 at 765.596 kb on - strand, within RR42_RS24245 at 765.596 kb on - strand, within RR42_RS24245 at 765.632 kb on + strand, within RR42_RS24245 at 765.633 kb on - strand, within RR42_RS24245 at 765.634 kb on + strand, within RR42_RS24245 at 765.657 kb on - strand, within RR42_RS24245 at 765.657 kb on - strand, within RR42_RS24245 at 765.660 kb on + strand, within RR42_RS24245 at 765.660 kb on + strand, within RR42_RS24245 at 765.660 kb on + strand, within RR42_RS24245 at 765.661 kb on - strand, within RR42_RS24245 at 765.661 kb on - strand, within RR42_RS24245 at 765.661 kb on - strand, within RR42_RS24245 at 765.696 kb on + strand, within RR42_RS24245 at 765.731 kb on + strand, within RR42_RS24245 at 765.732 kb on - strand, within RR42_RS24245 at 765.735 kb on + strand, within RR42_RS24245 at 765.840 kb on + strand, within RR42_RS24245 at 765.840 kb on + strand, within RR42_RS24245 at 765.841 kb on - strand, within RR42_RS24245 at 765.935 kb on + strand, within RR42_RS24245 at 765.935 kb on + strand, within RR42_RS24245 at 765.935 kb on + strand, within RR42_RS24245 at 765.958 kb on - strand, within RR42_RS24245 at 765.966 kb on + strand, within RR42_RS24245 at 765.966 kb on + strand, within RR42_RS24245 at 765.967 kb on - strand, within RR42_RS24245 at 765.967 kb on - strand, within RR42_RS24245 at 765.967 kb on - strand, within RR42_RS24245 at 765.971 kb on + strand, within RR42_RS24245 at 766.028 kb on + strand, within RR42_RS24245 at 766.028 kb on + strand, within RR42_RS24245 at 766.038 kb on + strand, within RR42_RS24245 at 766.038 kb on + strand, within RR42_RS24245 at 766.039 kb on - strand, within RR42_RS24245 at 766.039 kb on - strand, within RR42_RS24245 at 766.095 kb on + strand, within RR42_RS24245 at 766.095 kb on + strand, within RR42_RS24245 at 766.095 kb on + strand, within RR42_RS24245 at 766.158 kb on + strand, within RR42_RS24245 at 766.158 kb on + strand, within RR42_RS24245 at 766.159 kb on - strand, within RR42_RS24245 at 766.159 kb on - strand, within RR42_RS24245 at 766.159 kb on - strand, within RR42_RS24245 at 766.159 kb on - strand, within RR42_RS24245 at 766.176 kb on - strand, within RR42_RS24245 at 766.185 kb on + strand, within RR42_RS24245 at 766.186 kb on - strand, within RR42_RS24245 at 766.186 kb on - strand, within RR42_RS24245 at 766.229 kb on + strand at 766.229 kb on + strand at 766.229 kb on + strand at 766.230 kb on - strand at 766.249 kb on + strand at 766.253 kb on + strand at 766.308 kb on - strand at 766.308 kb on - strand at 766.415 kb on + strand at 766.428 kb on + strand at 766.429 kb on - strand at 766.429 kb on - strand at 766.429 kb on - strand at 766.445 kb on + strand at 766.577 kb on + strand at 766.589 kb on - strand at 766.600 kb on - strand at 766.600 kb on - strand at 766.600 kb on - strand at 766.779 kb on + strand, within RR42_RS24250 at 766.814 kb on + strand, within RR42_RS24250 at 766.814 kb on + strand, within RR42_RS24250 at 766.815 kb on - strand, within RR42_RS24250 at 766.858 kb on + strand, within RR42_RS24250 at 766.859 kb on - strand, within RR42_RS24250 at 766.859 kb on - strand, within RR42_RS24250 at 766.863 kb on - strand, within RR42_RS24250 at 766.988 kb on + strand, within RR42_RS24250 at 766.988 kb on + strand, within RR42_RS24250 at 767.018 kb on - strand, within RR42_RS24250 at 767.118 kb on - strand, within RR42_RS24250 at 767.252 kb on + strand, within RR42_RS24250 at 767.339 kb on + strand, within RR42_RS24250 at 767.339 kb on + strand, within RR42_RS24250 at 767.340 kb on - strand, within RR42_RS24250 at 767.367 kb on + strand, within RR42_RS24250 at 767.367 kb on + strand, within RR42_RS24250 at 767.367 kb on + strand, within RR42_RS24250 at 767.367 kb on + strand, within RR42_RS24250 at 767.368 kb on - strand, within RR42_RS24250 at 767.370 kb on + strand, within RR42_RS24250 at 767.371 kb on - strand, within RR42_RS24250 at 767.371 kb on - strand, within RR42_RS24250 at 767.391 kb on + strand, within RR42_RS24250 at 767.391 kb on + strand, within RR42_RS24250 at 767.391 kb on + strand, within RR42_RS24250 at 767.392 kb on - strand, within RR42_RS24250 at 767.392 kb on - strand, within RR42_RS24250 at 767.499 kb on + strand, within RR42_RS24250 at 767.525 kb on + strand, within RR42_RS24250 at 767.525 kb on + strand, within RR42_RS24250 at 767.525 kb on + strand, within RR42_RS24250 at 767.526 kb on - strand, within RR42_RS24250 at 767.526 kb on - strand, within RR42_RS24250 at 767.526 kb on - strand, within RR42_RS24250 at 767.528 kb on + strand, within RR42_RS24250 at 767.528 kb on + strand, within RR42_RS24250 at 767.625 kb on - strand at 767.625 kb on - strand at 767.671 kb on + strand at 767.672 kb on - strand at 767.672 kb on - strand at 767.672 kb on - strand at 767.706 kb on + strand at 767.706 kb on + strand at 767.706 kb on + strand at 767.706 kb on + strand at 767.706 kb on + strand at 767.707 kb on - strand at 767.707 kb on - strand at 767.707 kb on - strand at 767.707 kb on - strand at 767.718 kb on + strand at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.815 kb on - strand, within RR42_RS24255 at 767.823 kb on - strand, within RR42_RS24255 at 768.019 kb on + strand, within RR42_RS24255 at 768.020 kb on - strand, within RR42_RS24255 at 768.080 kb on + strand, within RR42_RS24255 at 768.081 kb on - strand, within RR42_RS24255 at 768.155 kb on + strand, within RR42_RS24255 at 768.155 kb on + strand, within RR42_RS24255 at 768.155 kb on + strand, within RR42_RS24255 at 768.155 kb on + strand, within RR42_RS24255 at 768.156 kb on - strand, within RR42_RS24255 at 768.203 kb on - strand, within RR42_RS24255 at 768.227 kb on + strand, within RR42_RS24255 at 768.227 kb on + strand, within RR42_RS24255 at 768.227 kb on + strand, within RR42_RS24255 at 768.227 kb on + strand, within RR42_RS24255 at 768.227 kb on + strand, within RR42_RS24255 at 768.446 kb on + strand, within RR42_RS24255 at 768.446 kb on + strand, within RR42_RS24255 at 768.447 kb on - strand, within RR42_RS24255 at 768.447 kb on - strand, within RR42_RS24255 at 768.447 kb on - strand, within RR42_RS24255 at 768.447 kb on - strand, within RR42_RS24255 at 768.525 kb on + strand at 768.525 kb on + strand at 768.526 kb on - strand at 768.564 kb on + strand at 768.564 kb on + strand at 768.564 kb on + strand at 768.564 kb on + strand at 768.565 kb on - strand at 768.565 kb on - strand at 768.565 kb on - strand at 768.565 kb on - strand at 768.583 kb on + strand at 768.584 kb on - strand at 768.586 kb on - strand at 768.617 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 765,586 - RR42_RS24245 0.31 +0.5 765,595 + RR42_RS24245 0.32 +1.0 765,595 + RR42_RS24245 0.32 +1.0 765,595 + RR42_RS24245 0.32 -0.5 765,596 - RR42_RS24245 0.32 +0.2 765,596 - RR42_RS24245 0.32 -2.6 765,632 + RR42_RS24245 0.35 -1.3 765,633 - RR42_RS24245 0.35 -1.2 765,634 + RR42_RS24245 0.35 -0.2 765,657 - RR42_RS24245 0.38 -0.1 765,657 - RR42_RS24245 0.38 -0.7 765,660 + RR42_RS24245 0.38 +0.3 765,660 + RR42_RS24245 0.38 +1.3 765,660 + RR42_RS24245 0.38 +0.8 765,661 - RR42_RS24245 0.38 -0.9 765,661 - RR42_RS24245 0.38 -1.1 765,661 - RR42_RS24245 0.38 -0.2 765,696 + RR42_RS24245 0.41 +1.6 765,731 + RR42_RS24245 0.45 +1.6 765,732 - RR42_RS24245 0.45 -0.1 765,735 + RR42_RS24245 0.45 -0.4 765,840 + RR42_RS24245 0.55 +1.1 765,840 + RR42_RS24245 0.55 -0.5 765,841 - RR42_RS24245 0.55 +2.3 765,935 + RR42_RS24245 0.64 -1.1 765,935 + RR42_RS24245 0.64 -0.7 765,935 + RR42_RS24245 0.64 -0.2 765,958 - RR42_RS24245 0.66 +0.1 765,966 + RR42_RS24245 0.67 -0.4 765,966 + RR42_RS24245 0.67 +0.2 765,967 - RR42_RS24245 0.67 -0.9 765,967 - RR42_RS24245 0.67 -0.7 765,967 - RR42_RS24245 0.67 -0.3 765,971 + RR42_RS24245 0.67 +0.8 766,028 + RR42_RS24245 0.72 +0.2 766,028 + RR42_RS24245 0.72 -1.6 766,038 + RR42_RS24245 0.73 -0.7 766,038 + RR42_RS24245 0.73 +0.8 766,039 - RR42_RS24245 0.73 -0.3 766,039 - RR42_RS24245 0.73 -0.5 766,095 + RR42_RS24245 0.79 -0.1 766,095 + RR42_RS24245 0.79 +0.8 766,095 + RR42_RS24245 0.79 +0.5 766,158 + RR42_RS24245 0.85 +0.0 766,158 + RR42_RS24245 0.85 -1.5 766,159 - RR42_RS24245 0.85 +0.3 766,159 - RR42_RS24245 0.85 -1.7 766,159 - RR42_RS24245 0.85 +0.8 766,159 - RR42_RS24245 0.85 -0.1 766,176 - RR42_RS24245 0.86 -0.5 766,185 + RR42_RS24245 0.87 -0.7 766,186 - RR42_RS24245 0.87 -0.6 766,186 - RR42_RS24245 0.87 +1.5 766,229 + -0.5 766,229 + +2.1 766,229 + +1.0 766,230 - +0.1 766,249 + +1.0 766,253 + -0.2 766,308 - +1.4 766,308 - -0.2 766,415 + +0.0 766,428 + +0.0 766,429 - -2.2 766,429 - -0.5 766,429 - +1.5 766,445 + +0.2 766,577 + -1.0 766,589 - -0.7 766,600 - +0.2 766,600 - -1.1 766,600 - +0.4 766,779 + RR42_RS24250 0.18 -0.0 766,814 + RR42_RS24250 0.22 +0.7 766,814 + RR42_RS24250 0.22 +0.7 766,815 - RR42_RS24250 0.22 -1.9 766,858 + RR42_RS24250 0.26 -1.0 766,859 - RR42_RS24250 0.26 -0.7 766,859 - RR42_RS24250 0.26 -1.4 766,863 - RR42_RS24250 0.26 +2.3 766,988 + RR42_RS24250 0.38 -0.8 766,988 + RR42_RS24250 0.38 -0.4 767,018 - RR42_RS24250 0.40 -0.7 767,118 - RR42_RS24250 0.50 +1.8 767,252 + RR42_RS24250 0.62 -0.3 767,339 + RR42_RS24250 0.70 +0.8 767,339 + RR42_RS24250 0.70 -0.3 767,340 - RR42_RS24250 0.70 -1.0 767,367 + RR42_RS24250 0.72 -1.1 767,367 + RR42_RS24250 0.72 -0.1 767,367 + RR42_RS24250 0.72 -0.7 767,367 + RR42_RS24250 0.72 +1.8 767,368 - RR42_RS24250 0.73 -0.3 767,370 + RR42_RS24250 0.73 -1.0 767,371 - RR42_RS24250 0.73 -1.0 767,371 - RR42_RS24250 0.73 +1.3 767,391 + RR42_RS24250 0.75 -0.7 767,391 + RR42_RS24250 0.75 +0.1 767,391 + RR42_RS24250 0.75 +0.8 767,392 - RR42_RS24250 0.75 +0.8 767,392 - RR42_RS24250 0.75 +0.8 767,499 + RR42_RS24250 0.85 -1.6 767,525 + RR42_RS24250 0.87 -1.4 767,525 + RR42_RS24250 0.87 +1.5 767,525 + RR42_RS24250 0.87 +0.6 767,526 - RR42_RS24250 0.87 -0.1 767,526 - RR42_RS24250 0.87 -1.2 767,526 - RR42_RS24250 0.87 +2.3 767,528 + RR42_RS24250 0.87 -1.1 767,528 + RR42_RS24250 0.87 +0.3 767,625 - -0.6 767,625 - -0.9 767,671 + -2.9 767,672 - +0.2 767,672 - +1.6 767,672 - -0.2 767,706 + +0.8 767,706 + -0.6 767,706 + -0.9 767,706 + +0.2 767,706 + -0.5 767,707 - +0.8 767,707 - -0.9 767,707 - +1.0 767,707 - +1.2 767,718 + -0.2 767,815 - RR42_RS24255 0.16 +0.8 767,815 - RR42_RS24255 0.16 -0.6 767,815 - RR42_RS24255 0.16 -0.5 767,815 - RR42_RS24255 0.16 -0.7 767,815 - RR42_RS24255 0.16 +0.4 767,815 - RR42_RS24255 0.16 +0.2 767,815 - RR42_RS24255 0.16 -0.5 767,823 - RR42_RS24255 0.17 +1.0 768,019 + RR42_RS24255 0.37 -1.1 768,020 - RR42_RS24255 0.37 +0.8 768,080 + RR42_RS24255 0.44 +1.1 768,081 - RR42_RS24255 0.44 -1.7 768,155 + RR42_RS24255 0.51 +0.0 768,155 + RR42_RS24255 0.51 -1.0 768,155 + RR42_RS24255 0.51 -0.5 768,155 + RR42_RS24255 0.51 +0.0 768,156 - RR42_RS24255 0.52 -0.4 768,203 - RR42_RS24255 0.56 +0.5 768,227 + RR42_RS24255 0.59 -0.5 768,227 + RR42_RS24255 0.59 +0.8 768,227 + RR42_RS24255 0.59 +0.0 768,227 + RR42_RS24255 0.59 -1.1 768,227 + RR42_RS24255 0.59 +0.2 768,446 + RR42_RS24255 0.82 -1.7 768,446 + RR42_RS24255 0.82 +0.8 768,447 - RR42_RS24255 0.82 -0.4 768,447 - RR42_RS24255 0.82 +1.4 768,447 - RR42_RS24255 0.82 -1.6 768,447 - RR42_RS24255 0.82 +1.3 768,525 + -0.8 768,525 + -0.1 768,526 - +2.3 768,564 + +1.2 768,564 + -0.7 768,564 + +0.0 768,564 + +0.5 768,565 - +1.3 768,565 - +0.3 768,565 - +0.3 768,565 - +2.3 768,583 + +1.0 768,584 - -1.2 768,586 - +1.6 768,617 + +0.0
Or see this region's nucleotide sequence