Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23480 and RR42_RS23485 are separated by 152 nucleotides RR42_RS23485 and RR42_RS23490 are separated by 28 nucleotides RR42_RS23490 and RR42_RS23495 are separated by 25 nucleotides RR42_RS23495 and RR42_RS23500 are separated by 59 nucleotides
RR42_RS23480: RR42_RS23480 - 3-keto-5-aminohexanoate cleavage protein, at 594,357 to 595,190
_RS23480
RR42_RS23485: RR42_RS23485 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase, at 595,343 to 596,128
_RS23485
RR42_RS23490: RR42_RS23490 - NADH oxidase, at 596,157 to 597,176
_RS23490
RR42_RS23495: RR42_RS23495 - benzene 1,2-dioxygenase, at 597,202 to 597,684
_RS23495
RR42_RS23500: RR42_RS23500 - benzene 1,2-dioxygenase, at 597,744 to 599,132
_RS23500
Position (kb) 596
597
598 Strain fitness (log2 ratio) -2
-1
0
1
2 at 595.173 kb on - strand at 595.195 kb on - strand at 595.214 kb on + strand at 595.214 kb on + strand at 595.215 kb on - strand at 595.217 kb on - strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.389 kb on + strand at 595.390 kb on - strand at 595.390 kb on - strand at 595.497 kb on + strand, within RR42_RS23485 at 595.674 kb on + strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 595.675 kb on - strand, within RR42_RS23485 at 596.128 kb on + strand at 596.129 kb on - strand at 596.269 kb on + strand, within RR42_RS23490 at 596.269 kb on + strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.270 kb on - strand, within RR42_RS23490 at 596.324 kb on - strand, within RR42_RS23490 at 596.449 kb on + strand, within RR42_RS23490 at 596.449 kb on + strand, within RR42_RS23490 at 596.450 kb on - strand, within RR42_RS23490 at 596.450 kb on - strand, within RR42_RS23490 at 596.664 kb on + strand, within RR42_RS23490 at 596.665 kb on - strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.752 kb on + strand, within RR42_RS23490 at 596.753 kb on - strand, within RR42_RS23490 at 596.984 kb on + strand, within RR42_RS23490 at 596.985 kb on - strand, within RR42_RS23490 at 596.986 kb on + strand, within RR42_RS23490 at 596.987 kb on - strand, within RR42_RS23490 at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.169 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.212 kb on + strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.213 kb on - strand at 597.236 kb on + strand at 597.257 kb on + strand, within RR42_RS23495 at 597.303 kb on - strand, within RR42_RS23495 at 597.326 kb on + strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.327 kb on - strand, within RR42_RS23495 at 597.599 kb on + strand, within RR42_RS23495 at 597.818 kb on - strand at 598.045 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500 at 598.102 kb on + strand, within RR42_RS23500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 595,173 - +0.5 595,195 - +2.0 595,214 + +0.1 595,214 + -1.0 595,215 - +0.8 595,217 - +0.9 595,389 + +0.1 595,389 + -0.0 595,389 + -0.3 595,389 + +0.7 595,389 + -0.7 595,389 + +0.5 595,390 - +0.4 595,390 - -1.0 595,497 + RR42_RS23485 0.20 -1.3 595,674 + RR42_RS23485 0.42 -1.3 595,675 - RR42_RS23485 0.42 -0.7 595,675 - RR42_RS23485 0.42 -0.2 596,128 + +0.7 596,129 - +0.9 596,269 + RR42_RS23490 0.11 -0.1 596,269 + RR42_RS23490 0.11 +0.6 596,270 - RR42_RS23490 0.11 +0.6 596,270 - RR42_RS23490 0.11 +0.5 596,270 - RR42_RS23490 0.11 +0.2 596,324 - RR42_RS23490 0.16 -0.1 596,449 + RR42_RS23490 0.29 -0.9 596,449 + RR42_RS23490 0.29 +1.2 596,450 - RR42_RS23490 0.29 +0.3 596,450 - RR42_RS23490 0.29 -0.0 596,664 + RR42_RS23490 0.50 -0.7 596,665 - RR42_RS23490 0.50 -1.1 596,752 + RR42_RS23490 0.58 -1.7 596,752 + RR42_RS23490 0.58 -0.1 596,752 + RR42_RS23490 0.58 +2.0 596,752 + RR42_RS23490 0.58 +0.2 596,752 + RR42_RS23490 0.58 +0.3 596,753 - RR42_RS23490 0.58 +1.3 596,984 + RR42_RS23490 0.81 -1.6 596,985 - RR42_RS23490 0.81 -0.4 596,986 + RR42_RS23490 0.81 -1.0 596,987 - RR42_RS23490 0.81 -1.7 597,169 + +0.9 597,169 + -0.7 597,169 + +1.5 597,169 + +0.7 597,212 + +0.3 597,212 + +0.0 597,212 + +0.4 597,212 + -1.8 597,213 - +0.4 597,213 - +0.9 597,213 - +0.3 597,236 + +0.1 597,257 + RR42_RS23495 0.11 -0.5 597,303 - RR42_RS23495 0.21 -0.1 597,326 + RR42_RS23495 0.26 -1.7 597,327 - RR42_RS23495 0.26 -1.1 597,327 - RR42_RS23495 0.26 -0.2 597,599 + RR42_RS23495 0.82 +0.3 597,818 - +1.3 598,045 + RR42_RS23500 0.22 +1.2 598,102 + RR42_RS23500 0.26 +0.7 598,102 + RR42_RS23500 0.26 -1.0
Or see this region's nucleotide sequence