Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22860 and RR42_RS22865 are separated by 57 nucleotides RR42_RS22865 and RR42_RS22870 are separated by 311 nucleotides
RR42_RS22860: RR42_RS22860 - l-threonine 3-dehydrogenase, at 459,894 to 460,949
_RS22860
RR42_RS22865: RR42_RS22865 - 2-amino-3-ketobutyrate CoA ligase, at 461,007 to 462,209
_RS22865
RR42_RS22870: RR42_RS22870 - sugar ABC transporter substrate-binding protein, at 462,521 to 463,930
_RS22870
Position (kb) 461
462
463 Strain fitness (log2 ratio) -2
-1
0
1
2 at 460.064 kb on - strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.090 kb on + strand, within RR42_RS22860 at 460.091 kb on - strand, within RR42_RS22860 at 460.579 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.676 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.752 kb on + strand, within RR42_RS22860 at 460.753 kb on - strand, within RR42_RS22860 at 460.756 kb on + strand, within RR42_RS22860 at 460.757 kb on - strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 460.820 kb on + strand, within RR42_RS22860 at 461.079 kb on - strand at 461.153 kb on - strand, within RR42_RS22865 at 461.153 kb on - strand, within RR42_RS22865 at 461.249 kb on + strand, within RR42_RS22865 at 461.250 kb on - strand, within RR42_RS22865 at 461.452 kb on + strand, within RR42_RS22865 at 461.453 kb on - strand, within RR42_RS22865 at 462.055 kb on + strand, within RR42_RS22865 at 462.055 kb on + strand, within RR42_RS22865 at 462.055 kb on + strand, within RR42_RS22865 at 462.055 kb on + strand, within RR42_RS22865 at 462.056 kb on - strand, within RR42_RS22865 at 462.056 kb on - strand, within RR42_RS22865 at 462.079 kb on - strand, within RR42_RS22865 at 462.079 kb on - strand, within RR42_RS22865 at 462.176 kb on + strand at 462.177 kb on - strand at 462.357 kb on - strand at 462.534 kb on + strand at 462.534 kb on + strand at 462.535 kb on - strand at 462.564 kb on - strand at 462.770 kb on + strand, within RR42_RS22870 at 462.770 kb on + strand, within RR42_RS22870 at 462.770 kb on + strand, within RR42_RS22870 at 462.770 kb on + strand, within RR42_RS22870 at 462.771 kb on - strand, within RR42_RS22870 at 462.771 kb on - strand, within RR42_RS22870 at 462.771 kb on - strand, within RR42_RS22870 at 462.822 kb on + strand, within RR42_RS22870 at 462.822 kb on + strand, within RR42_RS22870 at 462.823 kb on - strand, within RR42_RS22870 at 462.914 kb on + strand, within RR42_RS22870 at 462.915 kb on - strand, within RR42_RS22870 at 462.947 kb on + strand, within RR42_RS22870 at 462.948 kb on - strand, within RR42_RS22870 at 463.001 kb on - strand, within RR42_RS22870 at 463.026 kb on - strand, within RR42_RS22870 at 463.026 kb on - strand, within RR42_RS22870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 460,064 - RR42_RS22860 0.16 -0.9 460,090 + RR42_RS22860 0.19 +1.4 460,090 + RR42_RS22860 0.19 +1.3 460,091 - RR42_RS22860 0.19 +0.4 460,579 + RR42_RS22860 0.65 +0.0 460,676 + RR42_RS22860 0.74 +0.5 460,676 + RR42_RS22860 0.74 -0.2 460,676 + RR42_RS22860 0.74 +1.5 460,752 + RR42_RS22860 0.81 +1.0 460,752 + RR42_RS22860 0.81 +0.8 460,752 + RR42_RS22860 0.81 +0.0 460,753 - RR42_RS22860 0.81 -1.5 460,756 + RR42_RS22860 0.82 +0.6 460,757 - RR42_RS22860 0.82 +1.6 460,820 + RR42_RS22860 0.88 -0.2 460,820 + RR42_RS22860 0.88 +2.4 461,079 - -0.2 461,153 - RR42_RS22865 0.12 -2.2 461,153 - RR42_RS22865 0.12 +0.8 461,249 + RR42_RS22865 0.20 +0.8 461,250 - RR42_RS22865 0.20 +2.4 461,452 + RR42_RS22865 0.37 +0.5 461,453 - RR42_RS22865 0.37 +0.8 462,055 + RR42_RS22865 0.87 +0.2 462,055 + RR42_RS22865 0.87 +0.4 462,055 + RR42_RS22865 0.87 +0.4 462,055 + RR42_RS22865 0.87 +1.0 462,056 - RR42_RS22865 0.87 -0.3 462,056 - RR42_RS22865 0.87 -0.1 462,079 - RR42_RS22865 0.89 +0.1 462,079 - RR42_RS22865 0.89 +0.4 462,176 + -0.5 462,177 - +1.0 462,357 - -0.9 462,534 + +1.0 462,534 + +0.8 462,535 - -0.4 462,564 - -1.2 462,770 + RR42_RS22870 0.18 +1.2 462,770 + RR42_RS22870 0.18 -0.5 462,770 + RR42_RS22870 0.18 +1.4 462,770 + RR42_RS22870 0.18 -0.5 462,771 - RR42_RS22870 0.18 +0.2 462,771 - RR42_RS22870 0.18 -0.9 462,771 - RR42_RS22870 0.18 -0.0 462,822 + RR42_RS22870 0.21 +1.7 462,822 + RR42_RS22870 0.21 -0.1 462,823 - RR42_RS22870 0.21 -0.6 462,914 + RR42_RS22870 0.28 +1.0 462,915 - RR42_RS22870 0.28 +0.9 462,947 + RR42_RS22870 0.30 +1.5 462,948 - RR42_RS22870 0.30 +0.2 463,001 - RR42_RS22870 0.34 +1.4 463,026 - RR42_RS22870 0.36 -0.2 463,026 - RR42_RS22870 0.36 -0.4
Or see this region's nucleotide sequence