Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22625 and RR42_RS22630 are separated by 71 nucleotides RR42_RS22630 and RR42_RS22635 are separated by 207 nucleotides
RR42_RS22625: RR42_RS22625 - thiolase, at 408,459 to 409,637
_RS22625
RR42_RS22630: RR42_RS22630 - 3-hydroxyacyl-CoA dehydrogenase, at 409,709 to 411,799
_RS22630
RR42_RS22635: RR42_RS22635 - membrane protein, at 412,007 to 413,080
_RS22635
Position (kb) 409
410
411
412 Strain fitness (log2 ratio) -1
0
1
2
3 at 408.793 kb on + strand, within RR42_RS22625 at 408.793 kb on + strand, within RR42_RS22625 at 408.794 kb on - strand, within RR42_RS22625 at 408.880 kb on - strand, within RR42_RS22625 at 408.891 kb on + strand, within RR42_RS22625 at 408.892 kb on - strand, within RR42_RS22625 at 408.919 kb on - strand, within RR42_RS22625 at 409.183 kb on - strand, within RR42_RS22625 at 409.183 kb on - strand, within RR42_RS22625 at 409.326 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.488 kb on + strand, within RR42_RS22625 at 409.489 kb on - strand, within RR42_RS22625 at 409.489 kb on - strand, within RR42_RS22625 at 409.610 kb on + strand at 409.611 kb on - strand at 409.651 kb on + strand at 410.726 kb on + strand, within RR42_RS22630 at 410.930 kb on - strand, within RR42_RS22630 at 411.164 kb on + strand, within RR42_RS22630 at 411.286 kb on + strand, within RR42_RS22630 at 411.286 kb on + strand, within RR42_RS22630 at 411.287 kb on - strand, within RR42_RS22630 at 411.404 kb on + strand, within RR42_RS22630 at 411.407 kb on + strand, within RR42_RS22630 at 411.614 kb on + strand at 411.614 kb on + strand at 411.614 kb on + strand at 411.867 kb on - strand at 411.867 kb on - strand at 411.872 kb on - strand at 412.341 kb on - strand, within RR42_RS22635 at 412.552 kb on + strand, within RR42_RS22635 at 412.552 kb on + strand, within RR42_RS22635 at 412.553 kb on - strand, within RR42_RS22635 at 412.562 kb on + strand, within RR42_RS22635
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 408,793 + RR42_RS22625 0.28 +1.0 408,793 + RR42_RS22625 0.28 -1.6 408,794 - RR42_RS22625 0.28 -0.6 408,880 - RR42_RS22625 0.36 +0.1 408,891 + RR42_RS22625 0.37 +0.6 408,892 - RR42_RS22625 0.37 -0.7 408,919 - RR42_RS22625 0.39 +0.7 409,183 - RR42_RS22625 0.61 +0.0 409,183 - RR42_RS22625 0.61 +3.4 409,326 + RR42_RS22625 0.74 +0.2 409,488 + RR42_RS22625 0.87 -0.1 409,488 + RR42_RS22625 0.87 -0.0 409,488 + RR42_RS22625 0.87 -0.3 409,489 - RR42_RS22625 0.87 -0.7 409,489 - RR42_RS22625 0.87 -0.2 409,610 + +0.9 409,611 - +1.0 409,651 + +1.0 410,726 + RR42_RS22630 0.49 -1.6 410,930 - RR42_RS22630 0.58 +0.4 411,164 + RR42_RS22630 0.70 +0.2 411,286 + RR42_RS22630 0.75 -1.0 411,286 + RR42_RS22630 0.75 +0.1 411,287 - RR42_RS22630 0.75 -0.9 411,404 + RR42_RS22630 0.81 -0.5 411,407 + RR42_RS22630 0.81 +0.4 411,614 + +1.4 411,614 + -0.1 411,614 + -0.1 411,867 - -0.2 411,867 - -0.2 411,872 - -0.9 412,341 - RR42_RS22635 0.31 +1.5 412,552 + RR42_RS22635 0.51 -0.5 412,552 + RR42_RS22635 0.51 +0.1 412,553 - RR42_RS22635 0.51 -0.2 412,562 + RR42_RS22635 0.52 -0.5
Or see this region's nucleotide sequence