Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21420 and RR42_RS21425 are separated by 149 nucleotides RR42_RS21425 and RR42_RS21430 are separated by 12 nucleotides
RR42_RS21420: RR42_RS21420 - acyl-CoA dehydrogenase, at 134,799 to 135,956
_RS21420
RR42_RS21425: RR42_RS21425 - hypothetical protein, at 136,106 to 136,963
_RS21425
RR42_RS21430: RR42_RS21430 - alpha-ketoglutarate permease, at 136,976 to 138,286
_RS21430
Position (kb)
136
137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 135.123 kb on + strand, within RR42_RS21420 at 135.123 kb on + strand, within RR42_RS21420 at 135.237 kb on + strand, within RR42_RS21420 at 135.237 kb on + strand, within RR42_RS21420 at 135.238 kb on - strand, within RR42_RS21420 at 135.238 kb on - strand, within RR42_RS21420 at 135.293 kb on + strand, within RR42_RS21420 at 135.530 kb on + strand, within RR42_RS21420 at 135.531 kb on - strand, within RR42_RS21420 at 135.570 kb on - strand, within RR42_RS21420 at 135.585 kb on + strand, within RR42_RS21420 at 135.586 kb on - strand, within RR42_RS21420 at 135.586 kb on - strand, within RR42_RS21420 at 135.656 kb on + strand, within RR42_RS21420 at 135.672 kb on + strand, within RR42_RS21420 at 135.840 kb on + strand, within RR42_RS21420 at 135.840 kb on + strand, within RR42_RS21420 at 135.841 kb on - strand at 135.842 kb on + strand at 135.842 kb on + strand at 135.843 kb on - strand at 135.853 kb on - strand at 135.881 kb on - strand at 135.881 kb on - strand at 135.898 kb on - strand at 136.020 kb on + strand at 136.139 kb on + strand at 136.289 kb on + strand, within RR42_RS21425 at 136.289 kb on + strand, within RR42_RS21425 at 136.290 kb on - strand, within RR42_RS21425 at 136.404 kb on - strand, within RR42_RS21425 at 136.436 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.481 kb on + strand, within RR42_RS21425 at 136.523 kb on + strand, within RR42_RS21425 at 136.523 kb on + strand, within RR42_RS21425 at 136.589 kb on + strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.590 kb on - strand, within RR42_RS21425 at 136.730 kb on + strand, within RR42_RS21425 at 136.730 kb on + strand, within RR42_RS21425 at 136.731 kb on - strand, within RR42_RS21425 at 136.731 kb on - strand, within RR42_RS21425 at 136.732 kb on + strand, within RR42_RS21425 at 136.732 kb on + strand, within RR42_RS21425 at 136.733 kb on - strand, within RR42_RS21425 at 136.995 kb on + strand at 136.996 kb on - strand at 137.059 kb on - strand at 137.064 kb on + strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.065 kb on - strand at 137.110 kb on - strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.148 kb on + strand, within RR42_RS21430 at 137.149 kb on - strand, within RR42_RS21430 at 137.223 kb on + strand, within RR42_RS21430 at 137.224 kb on - strand, within RR42_RS21430 at 137.224 kb on - strand, within RR42_RS21430 at 137.347 kb on + strand, within RR42_RS21430 at 137.347 kb on + strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.348 kb on - strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.406 kb on + strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.407 kb on - strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.433 kb on + strand, within RR42_RS21430 at 137.434 kb on - strand, within RR42_RS21430 at 137.434 kb on - strand, within RR42_RS21430 at 137.485 kb on + strand, within RR42_RS21430 at 137.485 kb on + strand, within RR42_RS21430 at 137.486 kb on - strand, within RR42_RS21430 at 137.486 kb on - strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.487 kb on + strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.488 kb on - strand, within RR42_RS21430 at 137.500 kb on + strand, within RR42_RS21430 at 137.502 kb on + strand, within RR42_RS21430 at 137.503 kb on - strand, within RR42_RS21430 at 137.503 kb on - strand, within RR42_RS21430 at 137.527 kb on + strand, within RR42_RS21430 at 137.527 kb on + strand, within RR42_RS21430 at 137.530 kb on - strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.557 kb on + strand, within RR42_RS21430 at 137.558 kb on - strand, within RR42_RS21430 at 137.558 kb on - strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.839 kb on + strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.840 kb on - strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.841 kb on + strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.842 kb on - strand, within RR42_RS21430 at 137.860 kb on - strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.937 kb on + strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430 at 137.938 kb on - strand, within RR42_RS21430
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 135,123 + RR42_RS21420 0.28 +0.2 135,123 + RR42_RS21420 0.28 -0.0 135,237 + RR42_RS21420 0.38 -0.2 135,237 + RR42_RS21420 0.38 -1.4 135,238 - RR42_RS21420 0.38 -0.9 135,238 - RR42_RS21420 0.38 +0.4 135,293 + RR42_RS21420 0.43 -0.9 135,530 + RR42_RS21420 0.63 -0.1 135,531 - RR42_RS21420 0.63 -1.2 135,570 - RR42_RS21420 0.67 +0.2 135,585 + RR42_RS21420 0.68 -0.3 135,586 - RR42_RS21420 0.68 +0.9 135,586 - RR42_RS21420 0.68 +0.3 135,656 + RR42_RS21420 0.74 -0.7 135,672 + RR42_RS21420 0.75 +0.5 135,840 + RR42_RS21420 0.90 +0.6 135,840 + RR42_RS21420 0.90 -0.6 135,841 - +0.0 135,842 + +0.8 135,842 + -0.6 135,843 - -1.0 135,853 - -0.1 135,881 - +0.1 135,881 - +0.1 135,898 - +0.8 136,020 + +0.1 136,139 + -1.3 136,289 + RR42_RS21425 0.21 +1.0 136,289 + RR42_RS21425 0.21 -1.5 136,290 - RR42_RS21425 0.21 -0.4 136,404 - RR42_RS21425 0.35 +0.3 136,436 + RR42_RS21425 0.38 +1.0 136,481 + RR42_RS21425 0.44 +0.4 136,481 + RR42_RS21425 0.44 -0.5 136,481 + RR42_RS21425 0.44 +0.8 136,481 + RR42_RS21425 0.44 -0.4 136,523 + RR42_RS21425 0.49 +0.7 136,523 + RR42_RS21425 0.49 -1.0 136,589 + RR42_RS21425 0.56 +1.2 136,590 - RR42_RS21425 0.56 +0.6 136,590 - RR42_RS21425 0.56 +1.1 136,590 - RR42_RS21425 0.56 -0.1 136,730 + RR42_RS21425 0.73 -0.7 136,730 + RR42_RS21425 0.73 -0.9 136,731 - RR42_RS21425 0.73 -0.9 136,731 - RR42_RS21425 0.73 +0.8 136,732 + RR42_RS21425 0.73 -0.1 136,732 + RR42_RS21425 0.73 +0.2 136,733 - RR42_RS21425 0.73 -0.7 136,995 + +0.3 136,996 - +1.0 137,059 - +0.7 137,064 + -0.4 137,065 - -0.2 137,065 - -1.0 137,065 - -0.3 137,065 - +1.2 137,110 - RR42_RS21430 0.10 -0.1 137,148 + RR42_RS21430 0.13 -0.3 137,148 + RR42_RS21430 0.13 +0.1 137,148 + RR42_RS21430 0.13 -0.3 137,149 - RR42_RS21430 0.13 +0.3 137,223 + RR42_RS21430 0.19 -1.6 137,224 - RR42_RS21430 0.19 -0.3 137,224 - RR42_RS21430 0.19 +0.2 137,347 + RR42_RS21430 0.28 -0.7 137,347 + RR42_RS21430 0.28 +0.4 137,348 - RR42_RS21430 0.28 +0.0 137,348 - RR42_RS21430 0.28 -0.7 137,348 - RR42_RS21430 0.28 -0.4 137,406 + RR42_RS21430 0.33 -0.3 137,406 + RR42_RS21430 0.33 -2.4 137,406 + RR42_RS21430 0.33 -1.7 137,406 + RR42_RS21430 0.33 +1.5 137,407 - RR42_RS21430 0.33 -0.6 137,407 - RR42_RS21430 0.33 +0.6 137,407 - RR42_RS21430 0.33 -0.0 137,407 - RR42_RS21430 0.33 -0.6 137,407 - RR42_RS21430 0.33 -0.8 137,407 - RR42_RS21430 0.33 -0.5 137,407 - RR42_RS21430 0.33 -0.1 137,433 + RR42_RS21430 0.35 +0.8 137,433 + RR42_RS21430 0.35 -2.8 137,433 + RR42_RS21430 0.35 -0.3 137,434 - RR42_RS21430 0.35 -1.3 137,434 - RR42_RS21430 0.35 +1.5 137,485 + RR42_RS21430 0.39 +1.2 137,485 + RR42_RS21430 0.39 -0.8 137,486 - RR42_RS21430 0.39 +0.4 137,486 - RR42_RS21430 0.39 -2.4 137,487 + RR42_RS21430 0.39 -1.4 137,487 + RR42_RS21430 0.39 -0.2 137,487 + RR42_RS21430 0.39 -1.7 137,487 + RR42_RS21430 0.39 -0.1 137,487 + RR42_RS21430 0.39 +0.6 137,487 + RR42_RS21430 0.39 -0.3 137,487 + RR42_RS21430 0.39 +1.9 137,487 + RR42_RS21430 0.39 -1.4 137,488 - RR42_RS21430 0.39 -0.2 137,488 - RR42_RS21430 0.39 +1.8 137,488 - RR42_RS21430 0.39 -1.0 137,488 - RR42_RS21430 0.39 +1.8 137,488 - RR42_RS21430 0.39 -0.4 137,500 + RR42_RS21430 0.40 +0.2 137,502 + RR42_RS21430 0.40 +0.2 137,503 - RR42_RS21430 0.40 -0.3 137,503 - RR42_RS21430 0.40 +0.1 137,527 + RR42_RS21430 0.42 +1.0 137,527 + RR42_RS21430 0.42 -1.9 137,530 - RR42_RS21430 0.42 +0.8 137,557 + RR42_RS21430 0.44 -1.6 137,557 + RR42_RS21430 0.44 +0.8 137,557 + RR42_RS21430 0.44 -0.6 137,558 - RR42_RS21430 0.44 -0.4 137,558 - RR42_RS21430 0.44 +1.3 137,839 + RR42_RS21430 0.66 +0.0 137,839 + RR42_RS21430 0.66 -1.0 137,839 + RR42_RS21430 0.66 -0.3 137,839 + RR42_RS21430 0.66 +2.4 137,839 + RR42_RS21430 0.66 -0.2 137,839 + RR42_RS21430 0.66 +1.4 137,840 - RR42_RS21430 0.66 +0.6 137,840 - RR42_RS21430 0.66 +0.6 137,840 - RR42_RS21430 0.66 -1.3 137,840 - RR42_RS21430 0.66 -2.9 137,841 + RR42_RS21430 0.66 -1.3 137,841 + RR42_RS21430 0.66 +0.6 137,841 + RR42_RS21430 0.66 -0.8 137,841 + RR42_RS21430 0.66 +1.3 137,841 + RR42_RS21430 0.66 +0.8 137,841 + RR42_RS21430 0.66 +1.0 137,842 - RR42_RS21430 0.66 -0.2 137,842 - RR42_RS21430 0.66 +1.9 137,842 - RR42_RS21430 0.66 -0.2 137,842 - RR42_RS21430 0.66 +0.1 137,842 - RR42_RS21430 0.66 -0.8 137,860 - RR42_RS21430 0.67 +1.1 137,937 + RR42_RS21430 0.73 -0.4 137,937 + RR42_RS21430 0.73 +0.5 137,938 - RR42_RS21430 0.73 +1.0 137,938 - RR42_RS21430 0.73 -2.3
Or see this region's nucleotide sequence