Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21255 and RR42_RS21260 are separated by 118 nucleotides RR42_RS21260 and RR42_RS21265 are separated by 106 nucleotides
RR42_RS21255: RR42_RS21255 - LysR family transcriptional regulator, at 94,069 to 94,947
_RS21255
RR42_RS21260: RR42_RS21260 - isovaleryl-CoA dehydrogenase, at 95,066 to 96,862
_RS21260
RR42_RS21265: RR42_RS21265 - ABC transporter substrate-binding protein, at 96,969 to 97,949
_RS21265
Position (kb) 95
96
97 Strain fitness (log2 ratio) -2
-1
0
1
2 at 94.142 kb on + strand at 94.142 kb on + strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.143 kb on - strand at 94.301 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.340 kb on + strand, within RR42_RS21255 at 94.341 kb on - strand, within RR42_RS21255 at 94.344 kb on - strand, within RR42_RS21255 at 94.471 kb on + strand, within RR42_RS21255 at 94.640 kb on + strand, within RR42_RS21255 at 94.641 kb on - strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.842 kb on + strand, within RR42_RS21255 at 94.925 kb on + strand at 94.962 kb on + strand at 95.010 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.013 kb on + strand at 95.014 kb on - strand at 95.027 kb on + strand at 95.060 kb on + strand at 95.061 kb on - strand at 95.061 kb on - strand at 95.198 kb on + strand at 95.198 kb on + strand at 95.199 kb on - strand at 95.287 kb on + strand, within RR42_RS21260 at 95.287 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.369 kb on + strand, within RR42_RS21260 at 95.474 kb on + strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.475 kb on - strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.486 kb on + strand, within RR42_RS21260 at 95.630 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.888 kb on + strand, within RR42_RS21260 at 95.969 kb on + strand, within RR42_RS21260 at 95.969 kb on + strand, within RR42_RS21260 at 96.251 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.353 kb on + strand, within RR42_RS21260 at 96.354 kb on - strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.355 kb on + strand, within RR42_RS21260 at 96.356 kb on - strand, within RR42_RS21260 at 96.644 kb on + strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.645 kb on - strand, within RR42_RS21260 at 96.868 kb on + strand at 96.868 kb on + strand at 96.952 kb on + strand at 96.953 kb on - strand at 96.953 kb on - strand at 97.194 kb on - strand, within RR42_RS21265 at 97.233 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.299 kb on + strand, within RR42_RS21265 at 97.300 kb on - strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.437 kb on + strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.438 kb on - strand, within RR42_RS21265 at 97.525 kb on - strand, within RR42_RS21265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 94,142 + -1.6 94,142 + -0.9 94,143 - +0.3 94,143 - +0.2 94,143 - -0.5 94,143 - -0.7 94,301 + RR42_RS21255 0.26 +0.5 94,340 + RR42_RS21255 0.31 +1.4 94,340 + RR42_RS21255 0.31 +0.4 94,341 - RR42_RS21255 0.31 -1.3 94,344 - RR42_RS21255 0.31 -0.2 94,471 + RR42_RS21255 0.46 -0.4 94,640 + RR42_RS21255 0.65 +0.4 94,641 - RR42_RS21255 0.65 +0.7 94,842 + RR42_RS21255 0.88 -0.0 94,842 + RR42_RS21255 0.88 +1.2 94,925 + +0.0 94,962 + -0.7 95,010 + -0.5 95,013 + -1.0 95,013 + +0.7 95,013 + -2.7 95,014 - -0.6 95,027 + -0.1 95,060 + -0.9 95,061 - +0.6 95,061 - +1.1 95,198 + -0.9 95,198 + -1.1 95,199 - +0.1 95,287 + RR42_RS21260 0.12 +0.4 95,287 + RR42_RS21260 0.12 -0.2 95,369 + RR42_RS21260 0.17 +0.8 95,369 + RR42_RS21260 0.17 +0.1 95,369 + RR42_RS21260 0.17 +0.3 95,474 + RR42_RS21260 0.23 +1.4 95,475 - RR42_RS21260 0.23 +0.1 95,475 - RR42_RS21260 0.23 -0.7 95,486 + RR42_RS21260 0.23 +1.1 95,486 + RR42_RS21260 0.23 +2.1 95,486 + RR42_RS21260 0.23 -2.2 95,486 + RR42_RS21260 0.23 -0.1 95,486 + RR42_RS21260 0.23 -0.3 95,630 + RR42_RS21260 0.31 +0.7 95,888 + RR42_RS21260 0.46 -1.0 95,888 + RR42_RS21260 0.46 +0.8 95,888 + RR42_RS21260 0.46 -0.9 95,969 + RR42_RS21260 0.50 -0.5 95,969 + RR42_RS21260 0.50 -1.3 96,251 + RR42_RS21260 0.66 -2.2 96,353 + RR42_RS21260 0.72 -1.3 96,353 + RR42_RS21260 0.72 -0.5 96,354 - RR42_RS21260 0.72 -0.3 96,355 + RR42_RS21260 0.72 -0.2 96,355 + RR42_RS21260 0.72 -0.5 96,356 - RR42_RS21260 0.72 +0.9 96,644 + RR42_RS21260 0.88 -1.4 96,645 - RR42_RS21260 0.88 +0.8 96,645 - RR42_RS21260 0.88 +0.1 96,868 + +0.6 96,868 + -0.7 96,952 + +0.3 96,953 - +1.3 96,953 - +0.8 97,194 - RR42_RS21265 0.23 -0.2 97,233 + RR42_RS21265 0.27 +1.0 97,299 + RR42_RS21265 0.34 +1.2 97,299 + RR42_RS21265 0.34 -0.2 97,299 + RR42_RS21265 0.34 -0.2 97,300 - RR42_RS21265 0.34 -1.4 97,437 + RR42_RS21265 0.48 +0.4 97,437 + RR42_RS21265 0.48 +1.4 97,437 + RR42_RS21265 0.48 -0.0 97,437 + RR42_RS21265 0.48 +1.8 97,437 + RR42_RS21265 0.48 +1.3 97,437 + RR42_RS21265 0.48 +0.4 97,437 + RR42_RS21265 0.48 -0.1 97,437 + RR42_RS21265 0.48 +0.1 97,437 + RR42_RS21265 0.48 -1.2 97,438 - RR42_RS21265 0.48 -1.0 97,438 - RR42_RS21265 0.48 +0.3 97,438 - RR42_RS21265 0.48 +0.4 97,438 - RR42_RS21265 0.48 -1.9 97,525 - RR42_RS21265 0.57 -2.2
Or see this region's nucleotide sequence