Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17240 and RR42_RS17245 are separated by 82 nucleotides RR42_RS17245 and RR42_RS17250 are separated by 212 nucleotides RR42_RS17250 and RR42_RS17255 are separated by 13 nucleotides RR42_RS17255 and RR42_RS17260 are separated by 53 nucleotides
RR42_RS17240: RR42_RS17240 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 3,732,658 to 3,733,176
_RS17240
RR42_RS17245: RR42_RS17245 - deoxynucleoside kinase, at 3,733,259 to 3,733,900
_RS17245
RR42_RS17250: RR42_RS17250 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,734,113 to 3,734,934
_RS17250
RR42_RS17255: RR42_RS17255 - ABC transporter permease, at 3,734,948 to 3,735,646
_RS17255
RR42_RS17260: RR42_RS17260 - ABC transporter ATP-binding protein, at 3,735,700 to 3,736,374
_RS17260
Position (kb)
3734
3735 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3733.222 kb on + strand at 3733.222 kb on + strand at 3733.222 kb on + strand at 3733.223 kb on - strand at 3733.223 kb on - strand at 3733.223 kb on - strand at 3733.223 kb on - strand at 3733.223 kb on - strand at 3733.223 kb on - strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.225 kb on + strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.226 kb on - strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.229 kb on + strand at 3733.230 kb on - strand at 3733.230 kb on - strand at 3733.230 kb on - strand at 3733.230 kb on - strand at 3733.230 kb on - strand at 3733.230 kb on - strand at 3733.397 kb on + strand, within RR42_RS17245 at 3733.400 kb on + strand, within RR42_RS17245 at 3733.541 kb on + strand, within RR42_RS17245 at 3733.581 kb on - strand, within RR42_RS17245 at 3733.581 kb on - strand, within RR42_RS17245 at 3733.624 kb on - strand, within RR42_RS17245 at 3733.638 kb on + strand, within RR42_RS17245 at 3733.638 kb on + strand, within RR42_RS17245 at 3733.719 kb on - strand, within RR42_RS17245 at 3733.743 kb on - strand, within RR42_RS17245 at 3733.763 kb on + strand, within RR42_RS17245 at 3733.763 kb on + strand, within RR42_RS17245 at 3733.764 kb on - strand, within RR42_RS17245 at 3733.764 kb on - strand, within RR42_RS17245 at 3733.834 kb on + strand, within RR42_RS17245 at 3733.835 kb on - strand, within RR42_RS17245 at 3733.839 kb on + strand at 3733.839 kb on + strand at 3733.839 kb on + strand at 3733.993 kb on + strand at 3735.089 kb on + strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.090 kb on - strand, within RR42_RS17255 at 3735.188 kb on + strand, within RR42_RS17255 at 3735.238 kb on + strand, within RR42_RS17255 at 3735.485 kb on - strand, within RR42_RS17255 at 3735.556 kb on + strand, within RR42_RS17255 at 3735.557 kb on - strand, within RR42_RS17255 at 3735.741 kb on - strand at 3735.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 3,733,222 + -1.3 3,733,222 + +0.1 3,733,222 + -0.3 3,733,223 - -0.0 3,733,223 - -0.7 3,733,223 - -0.1 3,733,223 - +0.4 3,733,223 - +0.2 3,733,223 - -1.1 3,733,225 + -0.9 3,733,225 + +0.7 3,733,225 + +1.1 3,733,225 + -0.9 3,733,225 + +1.1 3,733,225 + -1.6 3,733,225 + +1.1 3,733,225 + +0.5 3,733,226 - +0.1 3,733,226 - +0.6 3,733,226 - -2.5 3,733,226 - +0.8 3,733,226 - -0.3 3,733,226 - +0.2 3,733,226 - -1.7 3,733,226 - -1.3 3,733,229 + -0.3 3,733,229 + -0.5 3,733,229 + +1.2 3,733,229 + -0.3 3,733,229 + -0.6 3,733,229 + +0.0 3,733,229 + -0.8 3,733,229 + +0.4 3,733,230 - +0.1 3,733,230 - -0.2 3,733,230 - +0.6 3,733,230 - +0.3 3,733,230 - +0.8 3,733,230 - -0.9 3,733,397 + RR42_RS17245 0.21 -1.0 3,733,400 + RR42_RS17245 0.22 -1.0 3,733,541 + RR42_RS17245 0.44 -0.8 3,733,581 - RR42_RS17245 0.50 -0.0 3,733,581 - RR42_RS17245 0.50 -0.3 3,733,624 - RR42_RS17245 0.57 -1.7 3,733,638 + RR42_RS17245 0.59 +2.4 3,733,638 + RR42_RS17245 0.59 +0.2 3,733,719 - RR42_RS17245 0.72 -0.8 3,733,743 - RR42_RS17245 0.75 +1.4 3,733,763 + RR42_RS17245 0.79 -0.5 3,733,763 + RR42_RS17245 0.79 -2.4 3,733,764 - RR42_RS17245 0.79 +1.4 3,733,764 - RR42_RS17245 0.79 -0.2 3,733,834 + RR42_RS17245 0.90 +0.2 3,733,835 - RR42_RS17245 0.90 +0.4 3,733,839 + +0.8 3,733,839 + -0.6 3,733,839 + -0.1 3,733,993 + -0.4 3,735,089 + RR42_RS17255 0.20 +0.5 3,735,090 - RR42_RS17255 0.20 -0.0 3,735,090 - RR42_RS17255 0.20 -1.9 3,735,188 + RR42_RS17255 0.34 +1.7 3,735,238 + RR42_RS17255 0.41 -0.2 3,735,485 - RR42_RS17255 0.77 +0.8 3,735,556 + RR42_RS17255 0.87 +0.4 3,735,557 - RR42_RS17255 0.87 +0.3 3,735,741 - -1.7 3,735,763 - -0.1
Or see this region's nucleotide sequence