Experiment: R2A with Nickel (II) chloride 0.35 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt RR42_RS12945 and RR42_RS12950 are separated by 28 nucleotides RR42_RS12950 and RR42_RS12955 are separated by 1 nucleotides RR42_RS12955 and RR42_RS12960 are separated by 68 nucleotides RR42_RS12960 and RR42_RS12965 are separated by 12 nucleotides 
        RR42_RS12945: RR42_RS12945 - membrane protein, at 2,785,664 to 2,787,259 
        _RS12945 
         
        
        RR42_RS12950: RR42_RS12950 - membrane protein, at 2,787,288 to 2,788,034 
        _RS12950 
         
        
        RR42_RS12955: RR42_RS12955 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 2,788,036 to 2,789,139 
        _RS12955 
         
        
        RR42_RS12960: RR42_RS12960 - glycerol-3-phosphate transporter membrane protein, at 2,789,208 to 2,790,056 
        _RS12960 
         
        
        RR42_RS12965: RR42_RS12965 - glycerol-3-phosphate transporter permease, at 2,790,069 to 2,790,950 
        _RS12965 
         Position (kb) 2788 
2789 
2790 Strain fitness (log2 ratio) -1 
0 
1 
2 
3 
4 at 2787.120 kb on + strand at 2787.201 kb on + strand at 2787.202 kb on - strand at 2787.290 kb on - strand at 2787.386 kb on + strand, within RR42_RS12950 at 2787.387 kb on - strand, within RR42_RS12950 at 2787.388 kb on + strand, within RR42_RS12950 at 2787.388 kb on + strand, within RR42_RS12950 at 2787.720 kb on - strand, within RR42_RS12950 at 2787.721 kb on + strand, within RR42_RS12950 at 2787.721 kb on + strand, within RR42_RS12950 at 2787.722 kb on - strand, within RR42_RS12950 at 2787.722 kb on - strand, within RR42_RS12950 at 2788.012 kb on + strand at 2788.013 kb on - strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.131 kb on + strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.132 kb on - strand at 2788.178 kb on + strand, within RR42_RS12955 at 2788.179 kb on - strand, within RR42_RS12955 at 2788.179 kb on - strand, within RR42_RS12955 at 2788.185 kb on - strand, within RR42_RS12955 at 2788.261 kb on - strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.472 kb on + strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.473 kb on - strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.571 kb on + strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.572 kb on - strand, within RR42_RS12955 at 2788.691 kb on - strand, within RR42_RS12955 at 2788.691 kb on - strand, within RR42_RS12955 at 2788.851 kb on + strand, within RR42_RS12955 at 2788.851 kb on + strand, within RR42_RS12955 at 2788.852 kb on - strand, within RR42_RS12955 at 2788.869 kb on + strand, within RR42_RS12955 at 2788.869 kb on + strand, within RR42_RS12955 at 2788.878 kb on - strand, within RR42_RS12955 at 2788.953 kb on + strand, within RR42_RS12955 at 2788.953 kb on + strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2788.954 kb on - strand, within RR42_RS12955 at 2789.102 kb on + strand at 2789.103 kb on - strand at 2789.103 kb on - strand at 2789.114 kb on - strand at 2789.143 kb on + strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.144 kb on - strand at 2789.413 kb on + strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.429 kb on - strand, within RR42_RS12960 at 2789.623 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.659 kb on + strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.747 kb on - strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.884 kb on + strand, within RR42_RS12960 at 2789.974 kb on + strand at 2789.974 kb on + strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2789.975 kb on - strand at 2790.074 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction R2A with Nickel (II) chloride 0.35 mM remove 2,787,120 +  -0.9 2,787,201 +  +0.6 2,787,202 -  +0.5 2,787,290 -  +0.8 2,787,386 +  RR42_RS12950 0.13  +0.1 2,787,387 -  RR42_RS12950 0.13  +0.3 2,787,388 +  RR42_RS12950 0.13  +0.3 2,787,388 +  RR42_RS12950 0.13  -0.6 2,787,720 -  RR42_RS12950 0.58  -0.5 2,787,721 +  RR42_RS12950 0.58  +0.3 2,787,721 +  RR42_RS12950 0.58  -0.3 2,787,722 -  RR42_RS12950 0.58  +2.2 2,787,722 -  RR42_RS12950 0.58  -1.6 2,788,012 +  +0.5 2,788,013 -  +0.6 2,788,131 +  -0.8 2,788,131 +  -0.4 2,788,131 +  +0.3 2,788,131 +  +0.2 2,788,131 +  -0.5 2,788,131 +  -1.4 2,788,132 -  +0.4 2,788,132 -  -0.1 2,788,132 -  +0.9 2,788,132 -  +0.7 2,788,132 -  +0.0 2,788,178 +  RR42_RS12955 0.13  -0.5 2,788,179 -  RR42_RS12955 0.13  +0.6 2,788,179 -  RR42_RS12955 0.13  +0.8 2,788,185 -  RR42_RS12955 0.13  -0.7 2,788,261 -  RR42_RS12955 0.20  +0.9 2,788,472 +  RR42_RS12955 0.39  -0.3 2,788,472 +  RR42_RS12955 0.39  -0.2 2,788,472 +  RR42_RS12955 0.39  +0.4 2,788,473 -  RR42_RS12955 0.40  -0.5 2,788,473 -  RR42_RS12955 0.40  +0.9 2,788,473 -  RR42_RS12955 0.40  +0.7 2,788,571 +  RR42_RS12955 0.48  +0.1 2,788,571 +  RR42_RS12955 0.48  +0.7 2,788,571 +  +2.2 2,788,571 +  RR42_RS12955 0.48  +0.2 2,788,571 +  RR42_RS12955 0.48  +0.5 2,788,571 +  RR42_RS12955 0.48  -1.7 2,788,572 -  RR42_RS12955 0.49  +0.2 2,788,572 -  RR42_RS12955 0.49  +0.7 2,788,572 -  RR42_RS12955 0.49  -1.0 2,788,572 -  RR42_RS12955 0.49  +0.6 2,788,572 -  RR42_RS12955 0.49  +0.6 2,788,572 -  RR42_RS12955 0.49  -1.7 2,788,572 -  RR42_RS12955 0.49  +0.6 2,788,572 -  RR42_RS12955 0.49  -0.7 2,788,572 -  RR42_RS12955 0.49  -0.4 2,788,572 -  RR42_RS12955 0.49  +0.5 2,788,572 -  RR42_RS12955 0.49  +2.2 2,788,691 -  RR42_RS12955 0.59  +0.3 2,788,691 -  RR42_RS12955 0.59  +0.1 2,788,851 +  RR42_RS12955 0.74  +2.2 2,788,851 +  RR42_RS12955 0.74  +1.5 2,788,852 -  RR42_RS12955 0.74  +0.1 2,788,869 +  RR42_RS12955 0.75  -0.0 2,788,869 +  RR42_RS12955 0.75  +0.4 2,788,878 -  RR42_RS12955 0.76  +2.6 2,788,953 +  RR42_RS12955 0.83  +1.4 2,788,953 +  RR42_RS12955 0.83  +1.5 2,788,954 -  RR42_RS12955 0.83  -1.0 2,788,954 -  RR42_RS12955 0.83  -0.4 2,788,954 -  RR42_RS12955 0.83  +0.5 2,789,102 +  +1.9 2,789,103 -  -1.1 2,789,103 -  -0.2 2,789,114 -  -0.4 2,789,143 +  -0.6 2,789,144 -  -0.8 2,789,144 -  -1.1 2,789,144 -  -1.6 2,789,144 -  -1.5 2,789,144 -  +0.6 2,789,413 +  RR42_RS12960 0.24  -0.8 2,789,429 -  RR42_RS12960 0.26  +0.2 2,789,429 -  RR42_RS12960 0.26  +1.6 2,789,623 +  RR42_RS12960 0.49  +4.2 2,789,659 +  RR42_RS12960 0.53  +0.7 2,789,659 +  RR42_RS12960 0.53  -1.1 2,789,747 -  RR42_RS12960 0.63  +0.7 2,789,747 -  RR42_RS12960 0.63  -0.4 2,789,884 +  RR42_RS12960 0.80  +0.9 2,789,884 +  RR42_RS12960 0.80  +1.2 2,789,974 +  -0.1 2,789,974 +  +0.1 2,789,975 -  -0.3 2,789,975 -  -1.0 2,789,975 -  +0.4 2,789,975 -  +2.2 2,789,975 -  +0.5 2,789,975 -  -0.0 2,790,074 -  +0.2 
Or see this region's nucleotide sequence