Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12250

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12245 and RR42_RS12250 overlap by 4 nucleotidesRR42_RS12250 and RR42_RS12255 overlap by 4 nucleotidesRR42_RS12255 and RR42_RS12260 are separated by 1 nucleotides RR42_RS12245: RR42_RS12245 - transcriptional regulator, at 2,631,635 to 2,632,582 _RS12245 RR42_RS12250: RR42_RS12250 - sulfate ABC transporter ATP-binding protein, at 2,632,579 to 2,633,703 _RS12250 RR42_RS12255: RR42_RS12255 - sulfate/thiosulfate transporter permease subunit, at 2,633,700 to 2,634,641 _RS12255 RR42_RS12260: RR42_RS12260 - sulfate transporter, at 2,634,643 to 2,635,587 _RS12260 Position (kb) 2632 2633 2634Strain fitness (log2 ratio) -1 0 1 2 3at 2631.735 kb on + strand, within RR42_RS12245at 2631.841 kb on - strand, within RR42_RS12245at 2631.998 kb on - strand, within RR42_RS12245at 2632.090 kb on + strand, within RR42_RS12245at 2632.090 kb on + strand, within RR42_RS12245at 2632.091 kb on - strand, within RR42_RS12245at 2632.092 kb on + strand, within RR42_RS12245at 2632.092 kb on + strand, within RR42_RS12245at 2632.165 kb on + strand, within RR42_RS12245at 2632.228 kb on - strand, within RR42_RS12245at 2632.266 kb on + strand, within RR42_RS12245at 2632.266 kb on + strand, within RR42_RS12245at 2632.429 kb on - strand, within RR42_RS12245at 2632.501 kb on + strandat 2632.501 kb on + strandat 2632.501 kb on + strandat 2632.502 kb on - strandat 2632.503 kb on + strandat 2632.503 kb on + strandat 2632.503 kb on + strandat 2632.503 kb on + strandat 2632.504 kb on - strandat 2632.868 kb on + strand, within RR42_RS12250at 2632.868 kb on + strand, within RR42_RS12250at 2632.933 kb on - strand, within RR42_RS12250at 2633.030 kb on + strand, within RR42_RS12250at 2633.031 kb on - strand, within RR42_RS12250at 2633.785 kb on + strandat 2633.869 kb on + strand, within RR42_RS12255at 2633.869 kb on + strand, within RR42_RS12255at 2633.992 kb on + strand, within RR42_RS12255at 2633.992 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.244 kb on + strand, within RR42_RS12255at 2634.245 kb on - strand, within RR42_RS12255at 2634.245 kb on - strand, within RR42_RS12255at 2634.449 kb on - strand, within RR42_RS12255at 2634.449 kb on - strand, within RR42_RS12255at 2634.478 kb on + strand, within RR42_RS12255at 2634.479 kb on - strand, within RR42_RS12255at 2634.479 kb on - strand, within RR42_RS12255at 2634.548 kb on - strandat 2634.548 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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2,631,735 + RR42_RS12245 0.11 +0.6
2,631,841 - RR42_RS12245 0.22 -0.8
2,631,998 - RR42_RS12245 0.38 -0.3
2,632,090 + RR42_RS12245 0.48 +0.6
2,632,090 + RR42_RS12245 0.48 -0.7
2,632,091 - RR42_RS12245 0.48 +2.1
2,632,092 + RR42_RS12245 0.48 +0.2
2,632,092 + RR42_RS12245 0.48 +0.5
2,632,165 + RR42_RS12245 0.56 +1.0
2,632,228 - RR42_RS12245 0.63 +1.6
2,632,266 + RR42_RS12245 0.67 -0.5
2,632,266 + RR42_RS12245 0.67 -1.0
2,632,429 - RR42_RS12245 0.84 -0.8
2,632,501 + +0.6
2,632,501 + -1.2
2,632,501 + -0.9
2,632,502 - +0.1
2,632,503 + +0.4
2,632,503 + -0.2
2,632,503 + -0.1
2,632,503 + +0.1
2,632,504 - +3.1
2,632,868 + RR42_RS12250 0.26 +1.3
2,632,868 + RR42_RS12250 0.26 -1.2
2,632,933 - RR42_RS12250 0.31 +0.4
2,633,030 + RR42_RS12250 0.40 +2.1
2,633,031 - RR42_RS12250 0.40 -0.4
2,633,785 + -0.9
2,633,869 + RR42_RS12255 0.18 -0.2
2,633,869 + RR42_RS12255 0.18 +1.4
2,633,992 + RR42_RS12255 0.31 -0.7
2,633,992 + RR42_RS12255 0.31 -0.7
2,634,244 + RR42_RS12255 0.58 +1.1
2,634,244 + RR42_RS12255 0.58 -1.5
2,634,244 + RR42_RS12255 0.58 +0.1
2,634,244 + RR42_RS12255 0.58 +1.8
2,634,244 + RR42_RS12255 0.58 +0.1
2,634,244 + RR42_RS12255 0.58 +0.5
2,634,244 + RR42_RS12255 0.58 -1.3
2,634,245 - RR42_RS12255 0.58 +0.3
2,634,245 - RR42_RS12255 0.58 +1.7
2,634,449 - RR42_RS12255 0.80 +1.5
2,634,449 - RR42_RS12255 0.80 +2.1
2,634,478 + RR42_RS12255 0.83 +2.1
2,634,479 - RR42_RS12255 0.83 -0.2
2,634,479 - RR42_RS12255 0.83 +0.6
2,634,548 - +1.0
2,634,548 - +0.8

Or see this region's nucleotide sequence