Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12195

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12185 and RR42_RS12190 are separated by 123 nucleotidesRR42_RS12190 and RR42_RS12195 are separated by 100 nucleotidesRR42_RS12195 and RR42_RS12200 are separated by 423 nucleotides RR42_RS12185: RR42_RS12185 - LysR family transcriptional regulator, at 2,620,878 to 2,621,831 _RS12185 RR42_RS12190: RR42_RS12190 - universal stress protein UspA, at 2,621,955 to 2,622,389 _RS12190 RR42_RS12195: RR42_RS12195 - peptidase, at 2,622,490 to 2,623,158 _RS12195 RR42_RS12200: RR42_RS12200 - isocitrate lyase, at 2,623,582 to 2,624,874 _RS12200 Position (kb) 2622 2623 2624Strain fitness (log2 ratio) -2 -1 0 1 2at 2621.754 kb on + strandat 2621.754 kb on + strandat 2621.754 kb on + strandat 2621.754 kb on + strandat 2621.754 kb on + strandat 2621.755 kb on - strandat 2621.755 kb on - strandat 2621.830 kb on - strandat 2621.874 kb on + strandat 2621.874 kb on + strandat 2621.875 kb on - strandat 2621.875 kb on - strandat 2621.875 kb on - strandat 2621.875 kb on - strandat 2622.060 kb on + strand, within RR42_RS12190at 2622.060 kb on + strand, within RR42_RS12190at 2622.061 kb on - strand, within RR42_RS12190at 2622.078 kb on + strand, within RR42_RS12190at 2622.084 kb on + strand, within RR42_RS12190at 2622.085 kb on - strand, within RR42_RS12190at 2622.085 kb on - strand, within RR42_RS12190at 2622.085 kb on - strand, within RR42_RS12190at 2622.157 kb on + strand, within RR42_RS12190at 2622.158 kb on - strand, within RR42_RS12190at 2622.158 kb on - strand, within RR42_RS12190at 2622.159 kb on + strand, within RR42_RS12190at 2622.381 kb on + strandat 2622.381 kb on + strandat 2622.656 kb on + strand, within RR42_RS12195at 2622.656 kb on + strand, within RR42_RS12195at 2622.656 kb on + strand, within RR42_RS12195at 2622.797 kb on + strand, within RR42_RS12195at 2622.797 kb on + strand, within RR42_RS12195at 2622.905 kb on + strand, within RR42_RS12195at 2622.905 kb on + strand, within RR42_RS12195at 2622.906 kb on - strand, within RR42_RS12195at 2622.959 kb on + strand, within RR42_RS12195at 2622.960 kb on - strand, within RR42_RS12195at 2623.031 kb on + strand, within RR42_RS12195at 2623.032 kb on - strand, within RR42_RS12195at 2623.032 kb on - strand, within RR42_RS12195at 2623.359 kb on + strandat 2623.359 kb on + strandat 2623.388 kb on - strandat 2623.396 kb on + strandat 2623.490 kb on + strandat 2623.491 kb on - strandat 2623.686 kb on - strandat 2623.779 kb on + strand, within RR42_RS12200at 2623.779 kb on + strand, within RR42_RS12200at 2623.780 kb on - strand, within RR42_RS12200at 2623.780 kb on - strand, within RR42_RS12200at 2623.780 kb on - strand, within RR42_RS12200at 2623.794 kb on - strand, within RR42_RS12200at 2623.794 kb on - strand, within RR42_RS12200at 2623.799 kb on + strand, within RR42_RS12200at 2623.820 kb on + strand, within RR42_RS12200at 2623.952 kb on + strand, within RR42_RS12200at 2623.953 kb on - strand, within RR42_RS12200at 2623.953 kb on - strand, within RR42_RS12200at 2623.953 kb on - strand, within RR42_RS12200at 2624.063 kb on + strand, within RR42_RS12200at 2624.111 kb on + strand, within RR42_RS12200

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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2,621,754 + +2.5
2,621,754 + -0.6
2,621,754 + -0.3
2,621,754 + +1.3
2,621,754 + +0.4
2,621,755 - -1.1
2,621,755 - -0.6
2,621,830 - -0.3
2,621,874 + -1.1
2,621,874 + -0.2
2,621,875 - +2.1
2,621,875 - -0.5
2,621,875 - -1.2
2,621,875 - -1.3
2,622,060 + RR42_RS12190 0.24 -0.8
2,622,060 + RR42_RS12190 0.24 -1.4
2,622,061 - RR42_RS12190 0.24 -0.9
2,622,078 + RR42_RS12190 0.28 -0.3
2,622,084 + RR42_RS12190 0.30 -0.5
2,622,085 - RR42_RS12190 0.30 -0.9
2,622,085 - RR42_RS12190 0.30 -2.1
2,622,085 - RR42_RS12190 0.30 -0.6
2,622,157 + RR42_RS12190 0.46 -0.9
2,622,158 - RR42_RS12190 0.47 -1.8
2,622,158 - RR42_RS12190 0.47 -0.1
2,622,159 + RR42_RS12190 0.47 -1.7
2,622,381 + +0.1
2,622,381 + +0.3
2,622,656 + RR42_RS12195 0.25 +0.6
2,622,656 + RR42_RS12195 0.25 +1.9
2,622,656 + RR42_RS12195 0.25 +0.1
2,622,797 + RR42_RS12195 0.46 +0.0
2,622,797 + RR42_RS12195 0.46 -1.6
2,622,905 + RR42_RS12195 0.62 -0.7
2,622,905 + RR42_RS12195 0.62 +0.1
2,622,906 - RR42_RS12195 0.62 -1.2
2,622,959 + RR42_RS12195 0.70 -0.6
2,622,960 - RR42_RS12195 0.70 -0.5
2,623,031 + RR42_RS12195 0.81 -0.5
2,623,032 - RR42_RS12195 0.81 +0.2
2,623,032 - RR42_RS12195 0.81 -1.0
2,623,359 + -0.7
2,623,359 + +0.1
2,623,388 - +0.0
2,623,396 + +2.5
2,623,490 + -2.0
2,623,491 - +2.1
2,623,686 - +0.8
2,623,779 + RR42_RS12200 0.15 -0.4
2,623,779 + RR42_RS12200 0.15 -0.8
2,623,780 - RR42_RS12200 0.15 +0.4
2,623,780 - RR42_RS12200 0.15 -0.1
2,623,780 - RR42_RS12200 0.15 -0.8
2,623,794 - RR42_RS12200 0.16 +0.9
2,623,794 - RR42_RS12200 0.16 +0.5
2,623,799 + RR42_RS12200 0.17 -0.9
2,623,820 + RR42_RS12200 0.18 -0.7
2,623,952 + RR42_RS12200 0.29 -0.4
2,623,953 - RR42_RS12200 0.29 +1.8
2,623,953 - RR42_RS12200 0.29 +1.2
2,623,953 - RR42_RS12200 0.29 +0.9
2,624,063 + RR42_RS12200 0.37 +1.4
2,624,111 + RR42_RS12200 0.41 -0.1

Or see this region's nucleotide sequence