Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12185 and RR42_RS12190 are separated by 123 nucleotides RR42_RS12190 and RR42_RS12195 are separated by 100 nucleotides RR42_RS12195 and RR42_RS12200 are separated by 423 nucleotides
RR42_RS12185: RR42_RS12185 - LysR family transcriptional regulator, at 2,620,878 to 2,621,831
_RS12185
RR42_RS12190: RR42_RS12190 - universal stress protein UspA, at 2,621,955 to 2,622,389
_RS12190
RR42_RS12195: RR42_RS12195 - peptidase, at 2,622,490 to 2,623,158
_RS12195
RR42_RS12200: RR42_RS12200 - isocitrate lyase, at 2,623,582 to 2,624,874
_RS12200
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.754 kb on + strand at 2621.755 kb on - strand at 2621.755 kb on - strand at 2621.830 kb on - strand at 2621.874 kb on + strand at 2621.874 kb on + strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2621.875 kb on - strand at 2622.060 kb on + strand, within RR42_RS12190 at 2622.060 kb on + strand, within RR42_RS12190 at 2622.061 kb on - strand, within RR42_RS12190 at 2622.078 kb on + strand, within RR42_RS12190 at 2622.084 kb on + strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.085 kb on - strand, within RR42_RS12190 at 2622.157 kb on + strand, within RR42_RS12190 at 2622.158 kb on - strand, within RR42_RS12190 at 2622.158 kb on - strand, within RR42_RS12190 at 2622.159 kb on + strand, within RR42_RS12190 at 2622.381 kb on + strand at 2622.381 kb on + strand at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.656 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.797 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.905 kb on + strand, within RR42_RS12195 at 2622.906 kb on - strand, within RR42_RS12195 at 2622.959 kb on + strand, within RR42_RS12195 at 2622.960 kb on - strand, within RR42_RS12195 at 2623.031 kb on + strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.032 kb on - strand, within RR42_RS12195 at 2623.359 kb on + strand at 2623.359 kb on + strand at 2623.388 kb on - strand at 2623.396 kb on + strand at 2623.490 kb on + strand at 2623.491 kb on - strand at 2623.686 kb on - strand at 2623.779 kb on + strand, within RR42_RS12200 at 2623.779 kb on + strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.780 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.794 kb on - strand, within RR42_RS12200 at 2623.799 kb on + strand, within RR42_RS12200 at 2623.820 kb on + strand, within RR42_RS12200 at 2623.952 kb on + strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2623.953 kb on - strand, within RR42_RS12200 at 2624.063 kb on + strand, within RR42_RS12200 at 2624.111 kb on + strand, within RR42_RS12200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 2,621,754 + +2.5 2,621,754 + -0.6 2,621,754 + -0.3 2,621,754 + +1.3 2,621,754 + +0.4 2,621,755 - -1.1 2,621,755 - -0.6 2,621,830 - -0.3 2,621,874 + -1.1 2,621,874 + -0.2 2,621,875 - +2.1 2,621,875 - -0.5 2,621,875 - -1.2 2,621,875 - -1.3 2,622,060 + RR42_RS12190 0.24 -0.8 2,622,060 + RR42_RS12190 0.24 -1.4 2,622,061 - RR42_RS12190 0.24 -0.9 2,622,078 + RR42_RS12190 0.28 -0.3 2,622,084 + RR42_RS12190 0.30 -0.5 2,622,085 - RR42_RS12190 0.30 -0.9 2,622,085 - RR42_RS12190 0.30 -2.1 2,622,085 - RR42_RS12190 0.30 -0.6 2,622,157 + RR42_RS12190 0.46 -0.9 2,622,158 - RR42_RS12190 0.47 -1.8 2,622,158 - RR42_RS12190 0.47 -0.1 2,622,159 + RR42_RS12190 0.47 -1.7 2,622,381 + +0.1 2,622,381 + +0.3 2,622,656 + RR42_RS12195 0.25 +0.6 2,622,656 + RR42_RS12195 0.25 +1.9 2,622,656 + RR42_RS12195 0.25 +0.1 2,622,797 + RR42_RS12195 0.46 +0.0 2,622,797 + RR42_RS12195 0.46 -1.6 2,622,905 + RR42_RS12195 0.62 -0.7 2,622,905 + RR42_RS12195 0.62 +0.1 2,622,906 - RR42_RS12195 0.62 -1.2 2,622,959 + RR42_RS12195 0.70 -0.6 2,622,960 - RR42_RS12195 0.70 -0.5 2,623,031 + RR42_RS12195 0.81 -0.5 2,623,032 - RR42_RS12195 0.81 +0.2 2,623,032 - RR42_RS12195 0.81 -1.0 2,623,359 + -0.7 2,623,359 + +0.1 2,623,388 - +0.0 2,623,396 + +2.5 2,623,490 + -2.0 2,623,491 - +2.1 2,623,686 - +0.8 2,623,779 + RR42_RS12200 0.15 -0.4 2,623,779 + RR42_RS12200 0.15 -0.8 2,623,780 - RR42_RS12200 0.15 +0.4 2,623,780 - RR42_RS12200 0.15 -0.1 2,623,780 - RR42_RS12200 0.15 -0.8 2,623,794 - RR42_RS12200 0.16 +0.9 2,623,794 - RR42_RS12200 0.16 +0.5 2,623,799 + RR42_RS12200 0.17 -0.9 2,623,820 + RR42_RS12200 0.18 -0.7 2,623,952 + RR42_RS12200 0.29 -0.4 2,623,953 - RR42_RS12200 0.29 +1.8 2,623,953 - RR42_RS12200 0.29 +1.2 2,623,953 - RR42_RS12200 0.29 +0.9 2,624,063 + RR42_RS12200 0.37 +1.4 2,624,111 + RR42_RS12200 0.41 -0.1
Or see this region's nucleotide sequence