Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07645 and RR42_RS07650 are separated by 49 nucleotides RR42_RS07650 and RR42_RS07655 are separated by 155 nucleotides RR42_RS07655 and RR42_RS07660 are separated by 53 nucleotides
RR42_RS07645: RR42_RS07645 - acetyl-CoA acetyltransferase, at 1,637,768 to 1,638,952
_RS07645
RR42_RS07650: RR42_RS07650 - hypothetical protein, at 1,639,002 to 1,639,649
_RS07650
RR42_RS07655: RR42_RS07655 - cystathionine beta-lyase, at 1,639,805 to 1,640,983
_RS07655
RR42_RS07660: RR42_RS07660 - phosphoserine phosphatase, at 1,641,037 to 1,641,891
_RS07660
Position (kb)
1639
1640
1641 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1638.887 kb on + strand at 1638.997 kb on + strand at 1638.997 kb on + strand at 1638.998 kb on - strand at 1638.998 kb on - strand at 1639.153 kb on + strand, within RR42_RS07650 at 1639.153 kb on + strand, within RR42_RS07650 at 1639.154 kb on - strand, within RR42_RS07650 at 1639.154 kb on - strand, within RR42_RS07650 at 1639.257 kb on + strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.258 kb on - strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.275 kb on + strand, within RR42_RS07650 at 1639.356 kb on + strand, within RR42_RS07650 at 1639.357 kb on - strand, within RR42_RS07650 at 1639.357 kb on - strand, within RR42_RS07650 at 1639.604 kb on + strand at 1639.605 kb on - strand at 1639.605 kb on - strand at 1639.609 kb on - strand at 1639.609 kb on - strand at 1639.609 kb on - strand at 1639.714 kb on - strand at 1639.870 kb on + strand at 1639.871 kb on - strand at 1639.871 kb on - strand at 1639.943 kb on + strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.944 kb on - strand, within RR42_RS07655 at 1639.952 kb on + strand, within RR42_RS07655 at 1639.953 kb on - strand, within RR42_RS07655 at 1639.966 kb on + strand, within RR42_RS07655 at 1639.967 kb on - strand, within RR42_RS07655 at 1640.067 kb on - strand, within RR42_RS07655 at 1640.117 kb on + strand, within RR42_RS07655 at 1640.177 kb on + strand, within RR42_RS07655 at 1640.180 kb on + strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.181 kb on - strand, within RR42_RS07655 at 1640.335 kb on + strand, within RR42_RS07655 at 1640.336 kb on - strand, within RR42_RS07655 at 1640.357 kb on + strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.412 kb on - strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.433 kb on + strand, within RR42_RS07655 at 1640.531 kb on + strand, within RR42_RS07655 at 1640.739 kb on - strand, within RR42_RS07655 at 1640.739 kb on - strand, within RR42_RS07655 at 1640.816 kb on - strand, within RR42_RS07655 at 1640.909 kb on + strand at 1640.910 kb on - strand at 1640.910 kb on - strand at 1640.910 kb on - strand at 1641.970 kb on - strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.974 kb on + strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.975 kb on - strand at 1641.976 kb on + strand at 1641.976 kb on + strand at 1641.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,638,887 + -2.2 1,638,997 + -0.9 1,638,997 + +0.9 1,638,998 - -2.6 1,638,998 - -0.3 1,639,153 + RR42_RS07650 0.23 +0.0 1,639,153 + RR42_RS07650 0.23 +0.4 1,639,154 - RR42_RS07650 0.23 -0.6 1,639,154 - RR42_RS07650 0.23 +0.2 1,639,257 + RR42_RS07650 0.39 +0.6 1,639,258 - RR42_RS07650 0.40 +1.6 1,639,258 - RR42_RS07650 0.40 -0.8 1,639,258 - RR42_RS07650 0.40 +0.2 1,639,275 + RR42_RS07650 0.42 -0.8 1,639,275 + RR42_RS07650 0.42 +0.7 1,639,275 + RR42_RS07650 0.42 -0.6 1,639,275 + RR42_RS07650 0.42 -0.1 1,639,356 + RR42_RS07650 0.55 -1.8 1,639,357 - RR42_RS07650 0.55 -1.2 1,639,357 - RR42_RS07650 0.55 -1.6 1,639,604 + -0.4 1,639,605 - -0.9 1,639,605 - +1.6 1,639,609 - +0.0 1,639,609 - -1.8 1,639,609 - -0.6 1,639,714 - +2.2 1,639,870 + -0.1 1,639,871 - +0.7 1,639,871 - +2.6 1,639,943 + RR42_RS07655 0.12 +0.6 1,639,944 - RR42_RS07655 0.12 +0.6 1,639,944 - RR42_RS07655 0.12 -0.1 1,639,944 - RR42_RS07655 0.12 -1.0 1,639,952 + RR42_RS07655 0.12 -0.4 1,639,953 - RR42_RS07655 0.13 +0.2 1,639,966 + RR42_RS07655 0.14 -0.1 1,639,967 - RR42_RS07655 0.14 +0.7 1,640,067 - RR42_RS07655 0.22 +0.5 1,640,117 + RR42_RS07655 0.26 -1.0 1,640,177 + RR42_RS07655 0.32 +0.2 1,640,180 + RR42_RS07655 0.32 +0.5 1,640,181 - RR42_RS07655 0.32 -0.4 1,640,181 - RR42_RS07655 0.32 +0.9 1,640,181 - RR42_RS07655 0.32 -0.9 1,640,181 - RR42_RS07655 0.32 +0.8 1,640,335 + RR42_RS07655 0.45 -0.7 1,640,336 - RR42_RS07655 0.45 -0.5 1,640,357 + RR42_RS07655 0.47 -0.0 1,640,412 - RR42_RS07655 0.51 -1.7 1,640,412 - RR42_RS07655 0.51 -0.6 1,640,412 - RR42_RS07655 0.51 -0.4 1,640,412 - RR42_RS07655 0.51 -1.2 1,640,433 + RR42_RS07655 0.53 -0.8 1,640,433 + RR42_RS07655 0.53 +0.0 1,640,433 + RR42_RS07655 0.53 -2.5 1,640,531 + RR42_RS07655 0.62 -0.5 1,640,739 - RR42_RS07655 0.79 -0.4 1,640,739 - RR42_RS07655 0.79 +0.2 1,640,816 - RR42_RS07655 0.86 +0.5 1,640,909 + +0.7 1,640,910 - -0.9 1,640,910 - -0.2 1,640,910 - -1.2 1,641,970 - -0.7 1,641,974 + -1.3 1,641,974 + -0.4 1,641,974 + -1.1 1,641,974 + +0.6 1,641,974 + +1.4 1,641,975 - +0.2 1,641,975 - +2.2 1,641,975 - +1.4 1,641,975 - +0.4 1,641,975 - -0.5 1,641,975 - -0.5 1,641,975 - +0.5 1,641,975 - -0.0 1,641,975 - +0.3 1,641,975 - -0.1 1,641,975 - -0.4 1,641,976 + -0.4 1,641,976 + -0.5 1,641,977 - -0.9
Or see this region's nucleotide sequence