Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06420 and RR42_RS06425 are separated by 305 nucleotides RR42_RS06425 and RR42_RS06430 are separated by 85 nucleotides RR42_RS06430 and RR42_RS06435 are separated by 21 nucleotides
RR42_RS06420: RR42_RS06420 - DSBA oxidoreductase, at 1,406,715 to 1,407,365
_RS06420
RR42_RS06425: RR42_RS06425 - anhydrase, at 1,407,671 to 1,408,213
_RS06425
RR42_RS06430: RR42_RS06430 - hypothetical protein, at 1,408,299 to 1,408,529
_RS06430
RR42_RS06435: RR42_RS06435 - 2-hydroxyacid dehydrogenase, at 1,408,551 to 1,409,972
_RS06435
Position (kb)
1407
1408
1409 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1406.682 kb on - strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.742 kb on + strand at 1406.743 kb on - strand at 1406.835 kb on + strand, within RR42_RS06420 at 1406.836 kb on - strand, within RR42_RS06420 at 1406.985 kb on + strand, within RR42_RS06420 at 1406.986 kb on - strand, within RR42_RS06420 at 1406.986 kb on - strand, within RR42_RS06420 at 1407.103 kb on - strand, within RR42_RS06420 at 1407.139 kb on + strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.185 kb on - strand, within RR42_RS06420 at 1407.367 kb on - strand at 1407.668 kb on - strand at 1407.756 kb on + strand, within RR42_RS06425 at 1407.963 kb on + strand, within RR42_RS06425 at 1407.964 kb on - strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.088 kb on + strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.089 kb on - strand, within RR42_RS06425 at 1408.153 kb on + strand, within RR42_RS06425 at 1408.154 kb on - strand, within RR42_RS06425 at 1408.167 kb on - strand at 1408.239 kb on + strand at 1408.355 kb on + strand, within RR42_RS06430 at 1408.356 kb on - strand, within RR42_RS06430 at 1408.795 kb on + strand, within RR42_RS06435 at 1408.795 kb on + strand, within RR42_RS06435 at 1408.795 kb on + strand, within RR42_RS06435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,406,682 - -1.3 1,406,742 + +0.4 1,406,742 + +3.0 1,406,742 + +0.5 1,406,742 + -0.9 1,406,742 + +2.2 1,406,743 - +0.2 1,406,835 + RR42_RS06420 0.18 -0.2 1,406,836 - RR42_RS06420 0.19 -0.4 1,406,985 + RR42_RS06420 0.41 +0.2 1,406,986 - RR42_RS06420 0.42 -0.7 1,406,986 - RR42_RS06420 0.42 -0.7 1,407,103 - RR42_RS06420 0.60 +0.2 1,407,139 + RR42_RS06420 0.65 +2.9 1,407,185 - RR42_RS06420 0.72 -0.6 1,407,185 - RR42_RS06420 0.72 -1.3 1,407,185 - RR42_RS06420 0.72 -0.1 1,407,367 - +0.7 1,407,668 - +0.9 1,407,756 + RR42_RS06425 0.16 +1.0 1,407,963 + RR42_RS06425 0.54 +0.2 1,407,964 - RR42_RS06425 0.54 +0.7 1,408,088 + RR42_RS06425 0.77 -0.6 1,408,088 + RR42_RS06425 0.77 -0.3 1,408,089 - RR42_RS06425 0.77 -0.2 1,408,089 - RR42_RS06425 0.77 +0.2 1,408,089 - RR42_RS06425 0.77 +0.3 1,408,089 - RR42_RS06425 0.77 -0.0 1,408,153 + RR42_RS06425 0.89 -0.7 1,408,154 - RR42_RS06425 0.89 +0.1 1,408,167 - +0.4 1,408,239 + -0.5 1,408,355 + RR42_RS06430 0.24 -1.1 1,408,356 - RR42_RS06430 0.25 +0.6 1,408,795 + RR42_RS06435 0.17 +0.5 1,408,795 + RR42_RS06435 0.17 +1.2 1,408,795 + RR42_RS06435 0.17 +0.6
Or see this region's nucleotide sequence