Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS06015 and RR42_RS06020 are separated by 80 nucleotides RR42_RS06020 and RR42_RS06025 are separated by 68 nucleotides RR42_RS06025 and RR42_RS06030 are separated by 62 nucleotides RR42_RS06030 and RR42_RS06035 are separated by 31 nucleotides RR42_RS06035 and RR42_RS06040 are separated by 28 nucleotides
RR42_RS06015: RR42_RS06015 - recombinase, at 1,321,784 to 1,323,874
_RS06015
RR42_RS06020: RR42_RS06020 - Fe-S cluster assembly transcriptional regulator IscR, at 1,323,955 to 1,324,509
_RS06020
RR42_RS06025: RR42_RS06025 - cysteine desulfurase, at 1,324,578 to 1,325,795
_RS06025
RR42_RS06030: RR42_RS06030 - Fe-S cluster assembly scaffold IscU, at 1,325,858 to 1,326,259
_RS06030
RR42_RS06035: RR42_RS06035 - heme biosynthesis protein HemY, at 1,326,291 to 1,326,611
_RS06035
RR42_RS06040: RR42_RS06040 - GNAT family acetyltransferase, at 1,326,640 to 1,326,873
_RS06040
Position (kb) 1324
1325
1326 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1324.248 kb on + strand, within RR42_RS06020 at 1325.828 kb on + strand at 1325.828 kb on + strand at 1325.832 kb on + strand at 1326.627 kb on + strand at 1326.720 kb on + strand, within RR42_RS06040 at 1326.720 kb on + strand, within RR42_RS06040
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 1,324,248 + RR42_RS06020 0.53 -2.4 1,325,828 + +1.4 1,325,828 + -0.9 1,325,832 + -0.5 1,326,627 + -0.5 1,326,720 + RR42_RS06040 0.34 +2.3 1,326,720 + RR42_RS06040 0.34 +0.4
Or see this region's nucleotide sequence