Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS04725

Experiment: R2A with Nickel (II) chloride 0.35 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS04715 and RR42_RS04720 are separated by 15 nucleotidesRR42_RS04720 and RR42_RS04725 are separated by 10 nucleotidesRR42_RS04725 and RR42_RS04730 are separated by 222 nucleotides RR42_RS04715: RR42_RS04715 - ribosome silencing factor RsfS, at 1,049,937 to 1,050,695 _RS04715 RR42_RS04720: RR42_RS04720 - nicotinate-nucleotide adenylyltransferase, at 1,050,711 to 1,051,481 _RS04720 RR42_RS04725: RR42_RS04725 - coproporphyrinogen III oxidase, at 1,051,492 to 1,052,403 _RS04725 RR42_RS04730: RR42_RS04730 - phosphoribosylamine--glycine ligase, at 1,052,626 to 1,053,894 _RS04730 Position (kb) 1051 1052 1053Strain fitness (log2 ratio) -2 -1 0 1 2at 1050.622 kb on + strandat 1050.623 kb on - strandat 1050.623 kb on - strandat 1050.689 kb on + strandat 1050.690 kb on - strandat 1052.407 kb on - strandat 1052.407 kb on - strandat 1052.490 kb on + strandat 1052.490 kb on + strandat 1052.490 kb on + strandat 1052.490 kb on + strandat 1052.491 kb on - strandat 1052.491 kb on - strandat 1052.524 kb on - strandat 1052.628 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM
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1,050,622 + +0.1
1,050,623 - -0.9
1,050,623 - +2.3
1,050,689 + -0.7
1,050,690 - +1.3
1,052,407 - +0.9
1,052,407 - +2.4
1,052,490 + -0.0
1,052,490 + -0.2
1,052,490 + -0.8
1,052,490 + +1.1
1,052,491 - +2.3
1,052,491 - -1.8
1,052,524 - +1.0
1,052,628 - -1.0

Or see this region's nucleotide sequence