Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01525 and RR42_RS01530 are separated by 53 nucleotides RR42_RS01530 and RR42_RS01535 are separated by 4 nucleotides RR42_RS01535 and RR42_RS01540 are separated by 13 nucleotides
RR42_RS01525: RR42_RS01525 - LacI family transcriptional regulator, at 343,426 to 344,427
_RS01525
RR42_RS01530: RR42_RS01530 - isopropylmalate isomerase, at 344,481 to 345,899
_RS01530
RR42_RS01535: RR42_RS01535 - 3-isopropylmalate dehydratase, at 345,904 to 346,551
_RS01535
RR42_RS01540: RR42_RS01540 - alpha/beta hydrolase, at 346,565 to 347,455
_RS01540
Position (kb) 344
345
346 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.005 kb on + strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.006 kb on - strand, within RR42_RS01525 at 344.191 kb on + strand, within RR42_RS01525 at 344.463 kb on + strand at 344.463 kb on + strand at 344.463 kb on + strand at 344.464 kb on - strand at 344.464 kb on - strand at 344.492 kb on + strand at 344.493 kb on - strand at 344.496 kb on + strand at 344.496 kb on + strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.497 kb on - strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.586 kb on + strand at 344.587 kb on - strand at 344.886 kb on + strand, within RR42_RS01530 at 344.964 kb on + strand, within RR42_RS01530 at 345.045 kb on - strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.210 kb on + strand, within RR42_RS01530 at 345.211 kb on - strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.459 kb on + strand, within RR42_RS01530 at 345.461 kb on + strand, within RR42_RS01530 at 345.462 kb on - strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.736 kb on + strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.737 kb on - strand, within RR42_RS01530 at 345.771 kb on - strand at 345.988 kb on - strand, within RR42_RS01535 at 345.988 kb on - strand, within RR42_RS01535 at 346.044 kb on + strand, within RR42_RS01535 at 346.187 kb on - strand, within RR42_RS01535 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.691 kb on - strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.793 kb on + strand, within RR42_RS01540 at 346.794 kb on - strand, within RR42_RS01540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 344,005 + RR42_RS01525 0.58 -0.8 344,005 + RR42_RS01525 0.58 -2.9 344,005 + RR42_RS01525 0.58 -0.3 344,005 + RR42_RS01525 0.58 -0.7 344,005 + RR42_RS01525 0.58 -1.5 344,005 + RR42_RS01525 0.58 -0.2 344,006 - RR42_RS01525 0.58 -1.6 344,006 - RR42_RS01525 0.58 +2.9 344,006 - RR42_RS01525 0.58 +1.3 344,006 - RR42_RS01525 0.58 +1.0 344,006 - RR42_RS01525 0.58 +0.3 344,006 - RR42_RS01525 0.58 -1.3 344,006 - RR42_RS01525 0.58 +0.4 344,191 + RR42_RS01525 0.76 +1.0 344,463 + +1.1 344,463 + -0.7 344,463 + +0.1 344,464 - +0.2 344,464 - -0.5 344,492 + +1.0 344,493 - -0.5 344,496 + +1.0 344,496 + -0.2 344,497 - -0.7 344,497 - +0.8 344,497 - -2.5 344,586 + +0.2 344,586 + -1.0 344,586 + -0.3 344,587 - -0.5 344,886 + RR42_RS01530 0.29 -0.4 344,964 + RR42_RS01530 0.34 +0.0 345,045 - RR42_RS01530 0.40 -0.5 345,210 + RR42_RS01530 0.51 -0.7 345,210 + RR42_RS01530 0.51 -0.3 345,211 - RR42_RS01530 0.51 -0.3 345,459 + RR42_RS01530 0.69 -0.7 345,459 + RR42_RS01530 0.69 -0.8 345,461 + RR42_RS01530 0.69 +0.0 345,462 - RR42_RS01530 0.69 -1.0 345,736 + RR42_RS01530 0.88 -0.7 345,736 + RR42_RS01530 0.88 -1.1 345,737 - RR42_RS01530 0.89 -1.1 345,737 - RR42_RS01530 0.89 -0.8 345,771 - +2.3 345,988 - RR42_RS01535 0.13 -0.1 345,988 - RR42_RS01535 0.13 +0.3 346,044 + RR42_RS01535 0.22 +0.2 346,187 - RR42_RS01535 0.44 +0.3 346,691 - RR42_RS01540 0.14 +0.0 346,691 - RR42_RS01540 0.14 -0.7 346,691 - RR42_RS01540 0.14 +1.0 346,793 + RR42_RS01540 0.26 +1.3 346,793 + RR42_RS01540 0.26 -0.1 346,793 + RR42_RS01540 0.26 +0.8 346,794 - RR42_RS01540 0.26 -0.3
Or see this region's nucleotide sequence