Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01495 and RR42_RS01500 are separated by 322 nucleotides RR42_RS01500 and RR42_RS01505 are separated by 149 nucleotides RR42_RS01505 and RR42_RS01510 are separated by 258 nucleotides
RR42_RS01495: RR42_RS01495 - diguanylate cyclase, at 337,571 to 339,352
_RS01495
RR42_RS01500: RR42_RS01500 - hypothetical protein, at 339,675 to 340,094
_RS01500
RR42_RS01505: RR42_RS01505 - tRNA-Cys, at 340,244 to 340,318
_RS01505
RR42_RS01510: RR42_RS01510 - N-acetylglutamate synthase, at 340,577 to 341,020
_RS01510
Position (kb) 339
340
341 Strain fitness (log2 ratio) -2
-1
0
1
2 at 338.820 kb on + strand, within RR42_RS01495 at 338.858 kb on + strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.131 kb on - strand, within RR42_RS01495 at 339.150 kb on + strand, within RR42_RS01495 at 339.275 kb on + strand at 339.275 kb on + strand at 339.275 kb on + strand at 339.336 kb on + strand at 339.337 kb on - strand at 339.337 kb on - strand at 339.340 kb on + strand at 339.341 kb on - strand at 339.482 kb on + strand at 339.482 kb on + strand at 339.482 kb on + strand at 339.519 kb on + strand at 339.562 kb on + strand at 339.563 kb on - strand at 339.633 kb on + strand at 339.642 kb on + strand at 339.912 kb on + strand, within RR42_RS01500 at 339.912 kb on + strand, within RR42_RS01500 at 339.913 kb on - strand, within RR42_RS01500 at 339.913 kb on - strand, within RR42_RS01500 at 340.161 kb on + strand at 340.162 kb on - strand at 340.162 kb on - strand at 340.285 kb on + strand, within RR42_RS01505 at 340.321 kb on - strand at 340.321 kb on - strand at 340.422 kb on - strand at 340.509 kb on + strand at 340.510 kb on - strand at 340.510 kb on - strand at 340.510 kb on - strand at 340.570 kb on - strand at 340.696 kb on + strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.780 kb on - strand, within RR42_RS01510 at 340.817 kb on - strand, within RR42_RS01510 at 340.817 kb on - strand, within RR42_RS01510 at 340.896 kb on - strand, within RR42_RS01510 at 340.896 kb on - strand, within RR42_RS01510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 338,820 + RR42_RS01495 0.70 -0.8 338,858 + RR42_RS01495 0.72 +1.8 339,131 - RR42_RS01495 0.88 -1.8 339,131 - RR42_RS01495 0.88 +0.7 339,131 - RR42_RS01495 0.88 -1.0 339,131 - RR42_RS01495 0.88 +0.4 339,150 + RR42_RS01495 0.89 +1.8 339,275 + +0.4 339,275 + -0.7 339,275 + -0.1 339,336 + -0.6 339,337 - -0.1 339,337 - +0.4 339,340 + +0.1 339,341 - -0.3 339,482 + +0.8 339,482 + -2.2 339,482 + -0.5 339,519 + +0.8 339,562 + +0.7 339,563 - -1.0 339,633 + +0.7 339,642 + +1.0 339,912 + RR42_RS01500 0.56 -0.6 339,912 + RR42_RS01500 0.56 +0.6 339,913 - RR42_RS01500 0.57 +0.6 339,913 - RR42_RS01500 0.57 +0.1 340,161 + +2.0 340,162 - -0.0 340,162 - -0.7 340,285 + RR42_RS01505 0.55 +0.1 340,321 - -0.5 340,321 - -1.4 340,422 - -0.1 340,509 + +0.8 340,510 - -0.9 340,510 - -0.9 340,510 - -0.7 340,570 - +0.3 340,696 + RR42_RS01510 0.27 -2.3 340,697 - RR42_RS01510 0.27 +0.3 340,697 - RR42_RS01510 0.27 -0.8 340,697 - RR42_RS01510 0.27 +2.3 340,780 - RR42_RS01510 0.46 -0.2 340,817 - RR42_RS01510 0.54 -1.0 340,817 - RR42_RS01510 0.54 +1.3 340,896 - RR42_RS01510 0.72 +0.6 340,896 - RR42_RS01510 0.72 +0.1
Or see this region's nucleotide sequence