Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00405 and RR42_RS00410 are separated by 121 nucleotides RR42_RS00410 and RR42_RS00415 are separated by 339 nucleotides RR42_RS00415 and RR42_RS00420 are separated by 128 nucleotides RR42_RS00420 and RR42_RS00425 are separated by 126 nucleotides
RR42_RS00405: RR42_RS00405 - glutamate carboxypeptidase, at 108,369 to 109,688
_RS00405
RR42_RS00410: RR42_RS00410 - transcriptional regulator, at 109,810 to 110,286
_RS00410
RR42_RS00415: RR42_RS00415 - ABC transporter permease, at 110,626 to 111,465
_RS00415
RR42_RS00420: RR42_RS00420 - hypothetical protein, at 111,594 to 111,884
_RS00420
RR42_RS00425: RR42_RS00425 - ABC transporter substrate-binding protein, at 112,011 to 113,024
_RS00425
Position (kb) 110
111
112 Strain fitness (log2 ratio) -1
0
1
2 at 109.674 kb on - strand at 109.674 kb on - strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.736 kb on + strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.737 kb on - strand at 109.921 kb on + strand, within RR42_RS00410 at 109.921 kb on + strand, within RR42_RS00410 at 109.922 kb on - strand, within RR42_RS00410 at 110.105 kb on + strand, within RR42_RS00410 at 110.106 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.200 kb on - strand, within RR42_RS00410 at 110.286 kb on + strand at 110.446 kb on + strand at 110.609 kb on + strand at 110.610 kb on - strand at 110.665 kb on + strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.666 kb on - strand at 110.818 kb on - strand, within RR42_RS00415 at 110.818 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.227 kb on - strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.262 kb on + strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.263 kb on - strand, within RR42_RS00415 at 111.362 kb on + strand, within RR42_RS00415 at 111.363 kb on - strand, within RR42_RS00415 at 111.496 kb on - strand at 111.616 kb on + strand at 111.616 kb on + strand at 111.796 kb on + strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.797 kb on - strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.807 kb on + strand, within RR42_RS00420 at 111.808 kb on - strand, within RR42_RS00420 at 111.883 kb on - strand at 112.129 kb on - strand, within RR42_RS00425 at 112.374 kb on + strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425 at 112.375 kb on - strand, within RR42_RS00425
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 109,674 - -0.1 109,674 - +0.1 109,736 + +0.1 109,736 + -0.7 109,736 + -1.2 109,737 - -1.7 109,737 - +2.1 109,737 - -0.2 109,921 + RR42_RS00410 0.23 +0.5 109,921 + RR42_RS00410 0.23 -0.6 109,922 - RR42_RS00410 0.23 +0.1 110,105 + RR42_RS00410 0.62 +0.4 110,106 - RR42_RS00410 0.62 +0.7 110,200 - RR42_RS00410 0.82 -0.8 110,200 - RR42_RS00410 0.82 +0.1 110,286 + +0.5 110,446 + -0.1 110,609 + -0.1 110,610 - +1.5 110,665 + +0.0 110,666 - -0.7 110,666 - -0.9 110,666 - +0.1 110,818 - RR42_RS00415 0.23 -0.2 110,818 - RR42_RS00415 0.23 +0.0 111,227 - RR42_RS00415 0.72 +1.5 111,227 - RR42_RS00415 0.72 -1.5 111,262 + RR42_RS00415 0.76 +0.9 111,262 + RR42_RS00415 0.76 -0.3 111,262 + RR42_RS00415 0.76 -0.1 111,263 - RR42_RS00415 0.76 +0.2 111,263 - RR42_RS00415 0.76 -0.8 111,263 - RR42_RS00415 0.76 +1.5 111,362 + RR42_RS00415 0.88 -0.6 111,363 - RR42_RS00415 0.88 -1.5 111,496 - -0.1 111,616 + +0.5 111,616 + -0.5 111,796 + RR42_RS00420 0.69 +1.0 111,797 - RR42_RS00420 0.70 -0.4 111,797 - RR42_RS00420 0.70 -0.0 111,807 + RR42_RS00420 0.73 +1.2 111,807 + RR42_RS00420 0.73 -0.3 111,808 - RR42_RS00420 0.74 -0.1 111,883 - -0.2 112,129 - RR42_RS00425 0.12 -0.4 112,374 + RR42_RS00425 0.36 -0.1 112,375 - RR42_RS00425 0.36 +0.5 112,375 - RR42_RS00425 0.36 -1.0 112,375 - RR42_RS00425 0.36 -0.0 112,375 - RR42_RS00425 0.36 -0.8
Or see this region's nucleotide sequence