Experiment: R2A with Nickel (II) chloride 0.35 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00190 and RR42_RS00195 are separated by 131 nucleotides RR42_RS00195 and RR42_RS00200 are separated by 58 nucleotides
RR42_RS00190: RR42_RS00190 - ABC transporter substrate-binding protein, at 62,998 to 64,179
_RS00190
RR42_RS00195: RR42_RS00195 - ABC transporter permease, at 64,311 to 65,240
_RS00195
RR42_RS00200: RR42_RS00200 - ABC transporter permease, at 65,299 to 66,429
_RS00200
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.412 kb on + strand, within RR42_RS00190 at 63.413 kb on - strand, within RR42_RS00190 at 63.420 kb on + strand, within RR42_RS00190 at 63.421 kb on - strand, within RR42_RS00190 at 63.421 kb on - strand, within RR42_RS00190 at 63.421 kb on - strand, within RR42_RS00190 at 63.503 kb on - strand, within RR42_RS00190 at 63.553 kb on - strand, within RR42_RS00190 at 63.559 kb on + strand, within RR42_RS00190 at 63.682 kb on + strand, within RR42_RS00190 at 63.683 kb on - strand, within RR42_RS00190 at 63.787 kb on + strand, within RR42_RS00190 at 63.787 kb on + strand, within RR42_RS00190 at 63.787 kb on + strand, within RR42_RS00190 at 63.788 kb on - strand, within RR42_RS00190 at 63.788 kb on - strand, within RR42_RS00190 at 63.835 kb on - strand, within RR42_RS00190 at 63.889 kb on + strand, within RR42_RS00190 at 63.889 kb on + strand, within RR42_RS00190 at 63.890 kb on - strand, within RR42_RS00190 at 63.925 kb on + strand, within RR42_RS00190 at 63.925 kb on + strand, within RR42_RS00190 at 64.099 kb on + strand at 64.099 kb on + strand at 64.099 kb on + strand at 64.099 kb on + strand at 64.100 kb on - strand at 64.100 kb on - strand at 64.100 kb on - strand at 64.100 kb on - strand at 64.144 kb on + strand at 64.178 kb on - strand at 64.329 kb on + strand at 64.330 kb on - strand at 64.363 kb on - strand at 64.385 kb on + strand at 64.385 kb on + strand at 64.472 kb on + strand, within RR42_RS00195 at 64.616 kb on + strand, within RR42_RS00195 at 64.616 kb on + strand, within RR42_RS00195 at 64.617 kb on - strand, within RR42_RS00195 at 64.823 kb on + strand, within RR42_RS00195 at 64.972 kb on - strand, within RR42_RS00195 at 65.149 kb on - strand at 65.335 kb on + strand at 65.335 kb on + strand at 65.336 kb on - strand at 65.524 kb on + strand, within RR42_RS00200 at 65.569 kb on + strand, within RR42_RS00200 at 65.569 kb on + strand, within RR42_RS00200 at 65.656 kb on - strand, within RR42_RS00200 at 65.656 kb on - strand, within RR42_RS00200 at 65.659 kb on + strand, within RR42_RS00200 at 65.660 kb on - strand, within RR42_RS00200 at 65.660 kb on - strand, within RR42_RS00200 at 65.785 kb on + strand, within RR42_RS00200 at 65.786 kb on - strand, within RR42_RS00200 at 65.900 kb on - strand, within RR42_RS00200 at 65.908 kb on - strand, within RR42_RS00200 at 65.956 kb on + strand, within RR42_RS00200 at 65.957 kb on - strand, within RR42_RS00200 at 65.957 kb on - strand, within RR42_RS00200 at 66.087 kb on + strand, within RR42_RS00200 at 66.154 kb on + strand, within RR42_RS00200 at 66.154 kb on + strand, within RR42_RS00200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.35 mM remove 63,412 + RR42_RS00190 0.35 -0.2 63,413 - RR42_RS00190 0.35 +0.7 63,420 + RR42_RS00190 0.36 -0.6 63,421 - RR42_RS00190 0.36 -1.0 63,421 - RR42_RS00190 0.36 +0.1 63,421 - RR42_RS00190 0.36 -2.2 63,503 - RR42_RS00190 0.43 -1.4 63,553 - RR42_RS00190 0.47 -0.2 63,559 + RR42_RS00190 0.47 +1.5 63,682 + RR42_RS00190 0.58 -0.3 63,683 - RR42_RS00190 0.58 -2.1 63,787 + RR42_RS00190 0.67 -1.7 63,787 + RR42_RS00190 0.67 -0.9 63,787 + RR42_RS00190 0.67 -0.8 63,788 - RR42_RS00190 0.67 +0.6 63,788 - RR42_RS00190 0.67 -0.3 63,835 - RR42_RS00190 0.71 -1.3 63,889 + RR42_RS00190 0.75 +0.1 63,889 + RR42_RS00190 0.75 +0.9 63,890 - RR42_RS00190 0.75 +0.9 63,925 + RR42_RS00190 0.78 -1.1 63,925 + RR42_RS00190 0.78 +0.8 64,099 + -0.7 64,099 + -0.6 64,099 + -0.1 64,099 + +1.5 64,100 - +0.2 64,100 - -2.5 64,100 - -0.1 64,100 - -0.9 64,144 + -0.4 64,178 - +0.5 64,329 + -2.4 64,330 - -0.5 64,363 - -0.3 64,385 + -0.6 64,385 + -0.3 64,472 + RR42_RS00195 0.17 -0.5 64,616 + RR42_RS00195 0.33 -0.7 64,616 + RR42_RS00195 0.33 +1.1 64,617 - RR42_RS00195 0.33 +1.3 64,823 + RR42_RS00195 0.55 +1.1 64,972 - RR42_RS00195 0.71 -0.1 65,149 - -0.4 65,335 + +0.5 65,335 + -1.0 65,336 - -1.9 65,524 + RR42_RS00200 0.20 -0.2 65,569 + RR42_RS00200 0.24 +0.3 65,569 + RR42_RS00200 0.24 -0.6 65,656 - RR42_RS00200 0.32 -0.5 65,656 - RR42_RS00200 0.32 +1.2 65,659 + RR42_RS00200 0.32 +0.4 65,660 - RR42_RS00200 0.32 -0.4 65,660 - RR42_RS00200 0.32 -0.3 65,785 + RR42_RS00200 0.43 -0.5 65,786 - RR42_RS00200 0.43 -0.5 65,900 - RR42_RS00200 0.53 +1.7 65,908 - RR42_RS00200 0.54 -0.8 65,956 + RR42_RS00200 0.58 -1.9 65,957 - RR42_RS00200 0.58 +0.5 65,957 - RR42_RS00200 0.58 -1.2 66,087 + RR42_RS00200 0.70 -0.6 66,154 + RR42_RS00200 0.76 -0.7 66,154 + RR42_RS00200 0.76 +2.1
Or see this region's nucleotide sequence