Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23490 and CA265_RS23495 are separated by 298 nucleotides CA265_RS23495 and CA265_RS23500 are separated by 79 nucleotides CA265_RS23500 and CA265_RS23505 are separated by 3 nucleotides
CA265_RS23490: CA265_RS23490 - phosphoenolpyruvate carboxykinase (ATP), at 5,745,725 to 5,747,323
_RS23490
CA265_RS23495: CA265_RS23495 - flagellar motor protein MotA, at 5,747,622 to 5,748,452
_RS23495
CA265_RS23500: CA265_RS23500 - biopolymer transporter ExbD, at 5,748,532 to 5,749,146
_RS23500
CA265_RS23505: CA265_RS23505 - biopolymer transporter ExbD, at 5,749,150 to 5,749,656
_RS23505
Position (kb)
5747
5748
5749 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5746.659 kb on - strand, within CA265_RS23490 at 5746.661 kb on - strand, within CA265_RS23490 at 5746.661 kb on - strand, within CA265_RS23490 at 5746.661 kb on - strand, within CA265_RS23490 at 5746.661 kb on - strand, within CA265_RS23490 at 5746.661 kb on - strand, within CA265_RS23490 at 5746.663 kb on + strand, within CA265_RS23490 at 5746.664 kb on - strand, within CA265_RS23490 at 5746.664 kb on - strand, within CA265_RS23490 at 5746.698 kb on - strand, within CA265_RS23490 at 5746.704 kb on + strand, within CA265_RS23490 at 5746.705 kb on - strand, within CA265_RS23490 at 5746.705 kb on - strand, within CA265_RS23490 at 5746.705 kb on - strand, within CA265_RS23490 at 5746.709 kb on + strand, within CA265_RS23490 at 5746.709 kb on + strand, within CA265_RS23490 at 5746.737 kb on + strand, within CA265_RS23490 at 5746.847 kb on + strand, within CA265_RS23490 at 5746.897 kb on - strand, within CA265_RS23490 at 5746.945 kb on + strand, within CA265_RS23490 at 5746.950 kb on - strand, within CA265_RS23490 at 5746.960 kb on - strand, within CA265_RS23490 at 5746.960 kb on - strand, within CA265_RS23490 at 5746.989 kb on + strand, within CA265_RS23490 at 5746.990 kb on - strand, within CA265_RS23490 at 5747.063 kb on - strand, within CA265_RS23490 at 5747.078 kb on - strand, within CA265_RS23490 at 5747.078 kb on - strand, within CA265_RS23490 at 5747.100 kb on + strand, within CA265_RS23490 at 5747.110 kb on + strand, within CA265_RS23490 at 5747.110 kb on + strand, within CA265_RS23490 at 5747.111 kb on - strand, within CA265_RS23490 at 5747.116 kb on + strand, within CA265_RS23490 at 5747.117 kb on - strand, within CA265_RS23490 at 5747.217 kb on - strand at 5747.223 kb on + strand at 5747.333 kb on - strand at 5747.427 kb on - strand at 5747.427 kb on - strand at 5747.542 kb on + strand at 5747.558 kb on + strand at 5747.561 kb on + strand at 5748.437 kb on + strand at 5748.437 kb on + strand at 5748.528 kb on + strand at 5749.160 kb on + strand at 5749.197 kb on + strand at 5749.217 kb on + strand, within CA265_RS23505 at 5749.218 kb on - strand, within CA265_RS23505 at 5749.260 kb on - strand, within CA265_RS23505 at 5749.387 kb on + strand, within CA265_RS23505 at 5749.449 kb on + strand, within CA265_RS23505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 5,746,659 - CA265_RS23490 0.58 +0.3 5,746,661 - CA265_RS23490 0.59 +0.8 5,746,661 - CA265_RS23490 0.59 +0.4 5,746,661 - CA265_RS23490 0.59 +0.3 5,746,661 - CA265_RS23490 0.59 -0.7 5,746,661 - CA265_RS23490 0.59 -0.2 5,746,663 + CA265_RS23490 0.59 +1.1 5,746,664 - CA265_RS23490 0.59 +0.8 5,746,664 - CA265_RS23490 0.59 +0.6 5,746,698 - CA265_RS23490 0.61 +0.9 5,746,704 + CA265_RS23490 0.61 +0.6 5,746,705 - CA265_RS23490 0.61 +0.6 5,746,705 - CA265_RS23490 0.61 +0.8 5,746,705 - CA265_RS23490 0.61 +0.0 5,746,709 + CA265_RS23490 0.62 +0.6 5,746,709 + CA265_RS23490 0.62 -1.6 5,746,737 + CA265_RS23490 0.63 +0.3 5,746,847 + CA265_RS23490 0.70 +0.6 5,746,897 - CA265_RS23490 0.73 +0.4 5,746,945 + CA265_RS23490 0.76 +0.5 5,746,950 - CA265_RS23490 0.77 -0.1 5,746,960 - CA265_RS23490 0.77 +0.6 5,746,960 - CA265_RS23490 0.77 -0.1 5,746,989 + CA265_RS23490 0.79 +1.0 5,746,990 - CA265_RS23490 0.79 +0.4 5,747,063 - CA265_RS23490 0.84 +0.1 5,747,078 - CA265_RS23490 0.85 +0.5 5,747,078 - CA265_RS23490 0.85 +0.4 5,747,100 + CA265_RS23490 0.86 +0.4 5,747,110 + CA265_RS23490 0.87 +0.8 5,747,110 + CA265_RS23490 0.87 +0.5 5,747,111 - CA265_RS23490 0.87 +0.6 5,747,116 + CA265_RS23490 0.87 +0.5 5,747,117 - CA265_RS23490 0.87 +0.4 5,747,217 - -1.3 5,747,223 + +0.0 5,747,333 - +1.2 5,747,427 - +0.8 5,747,427 - +1.5 5,747,542 + -1.5 5,747,558 + -4.7 5,747,561 + -2.8 5,748,437 + +0.2 5,748,437 + +1.2 5,748,528 + +2.3 5,749,160 + +2.4 5,749,197 + +2.4 5,749,217 + CA265_RS23505 0.13 +0.5 5,749,218 - CA265_RS23505 0.13 +2.6 5,749,260 - CA265_RS23505 0.22 +1.7 5,749,387 + CA265_RS23505 0.47 +2.0 5,749,449 + CA265_RS23505 0.59 +2.1
Or see this region's nucleotide sequence