Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21100 and CA265_RS21105 are separated by 61 nucleotides CA265_RS21105 and CA265_RS21110 are separated by 78 nucleotides CA265_RS21110 and CA265_RS21115 are separated by 43 nucleotides CA265_RS21115 and CA265_RS21120 overlap by 32 nucleotides
CA265_RS21100: CA265_RS21100 - acyl-CoA thioesterase, at 5,200,294 to 5,200,680
_RS21100
CA265_RS21105: CA265_RS21105 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase, at 5,200,742 to 5,201,407
_RS21105
CA265_RS21110: CA265_RS21110 - phosphatidate cytidylyltransferase, at 5,201,486 to 5,202,295
_RS21110
CA265_RS21115: CA265_RS21115 - hypothetical protein, at 5,202,339 to 5,202,557
_RS21115
CA265_RS21120: CA265_RS21120 - CPBP family intramembrane metalloprotease domain-containing protein, at 5,202,526 to 5,203,452
_RS21120
Position (kb)
5201
5202
5203 Strain fitness (log2 ratio)
-2
-1
0
1 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.509 kb on + strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.510 kb on - strand, within CA265_RS21100 at 5200.533 kb on - strand, within CA265_RS21100 at 5200.548 kb on + strand, within CA265_RS21100 at 5200.577 kb on - strand, within CA265_RS21100 at 5200.591 kb on + strand, within CA265_RS21100 at 5200.777 kb on + strand at 5200.847 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5200.948 kb on - strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.077 kb on + strand, within CA265_RS21105 at 5201.078 kb on - strand, within CA265_RS21105 at 5201.125 kb on + strand, within CA265_RS21105 at 5201.126 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.133 kb on - strand, within CA265_RS21105 at 5201.156 kb on + strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.157 kb on - strand, within CA265_RS21105 at 5201.245 kb on + strand, within CA265_RS21105 at 5201.292 kb on + strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.312 kb on - strand, within CA265_RS21105 at 5201.354 kb on - strand at 5201.381 kb on + strand at 5201.384 kb on + strand at 5201.392 kb on - strand at 5202.298 kb on - strand at 5202.326 kb on - strand at 5202.334 kb on + strand at 5202.334 kb on + strand at 5202.364 kb on + strand, within CA265_RS21115 at 5202.365 kb on - strand, within CA265_RS21115 at 5202.365 kb on - strand, within CA265_RS21115 at 5202.365 kb on - strand, within CA265_RS21115 at 5202.365 kb on - strand, within CA265_RS21115 at 5202.365 kb on - strand, within CA265_RS21115 at 5202.387 kb on + strand, within CA265_RS21115 at 5202.388 kb on - strand, within CA265_RS21115 at 5202.388 kb on - strand, within CA265_RS21115 at 5202.409 kb on - strand, within CA265_RS21115 at 5202.423 kb on - strand, within CA265_RS21115 at 5202.423 kb on - strand, within CA265_RS21115 at 5202.519 kb on - strand, within CA265_RS21115 at 5202.519 kb on - strand, within CA265_RS21115 at 5202.519 kb on - strand, within CA265_RS21115 at 5202.519 kb on - strand, within CA265_RS21115 at 5202.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 5,200,509 + CA265_RS21100 0.56 -1.7 5,200,509 + CA265_RS21100 0.56 -2.3 5,200,510 - CA265_RS21100 0.56 -0.5 5,200,510 - CA265_RS21100 0.56 -0.9 5,200,533 - CA265_RS21100 0.62 -0.4 5,200,548 + CA265_RS21100 0.66 +0.5 5,200,577 - CA265_RS21100 0.73 -1.1 5,200,591 + CA265_RS21100 0.77 -0.1 5,200,777 + +0.4 5,200,847 - CA265_RS21105 0.16 +0.1 5,200,948 - CA265_RS21105 0.31 +0.7 5,200,948 - CA265_RS21105 0.31 -0.2 5,201,077 + CA265_RS21105 0.50 -0.4 5,201,077 + CA265_RS21105 0.50 +0.0 5,201,078 - CA265_RS21105 0.50 -0.7 5,201,125 + CA265_RS21105 0.58 +0.3 5,201,126 - CA265_RS21105 0.58 -1.4 5,201,133 - CA265_RS21105 0.59 +0.1 5,201,133 - CA265_RS21105 0.59 +0.0 5,201,156 + CA265_RS21105 0.62 +0.0 5,201,157 - CA265_RS21105 0.62 -0.6 5,201,157 - CA265_RS21105 0.62 +1.0 5,201,245 + CA265_RS21105 0.76 -0.5 5,201,292 + CA265_RS21105 0.83 -0.3 5,201,312 - CA265_RS21105 0.86 +0.0 5,201,312 - CA265_RS21105 0.86 -0.0 5,201,354 - +0.6 5,201,381 + +0.3 5,201,384 + +0.3 5,201,392 - +1.2 5,202,298 - -0.8 5,202,326 - -0.6 5,202,334 + +0.5 5,202,334 + -1.0 5,202,364 + CA265_RS21115 0.11 +0.0 5,202,365 - CA265_RS21115 0.12 +0.2 5,202,365 - CA265_RS21115 0.12 +0.5 5,202,365 - CA265_RS21115 0.12 +0.1 5,202,365 - CA265_RS21115 0.12 +1.2 5,202,365 - CA265_RS21115 0.12 +1.3 5,202,387 + CA265_RS21115 0.22 -0.4 5,202,388 - CA265_RS21115 0.22 -0.3 5,202,388 - CA265_RS21115 0.22 -0.1 5,202,409 - CA265_RS21115 0.32 +0.2 5,202,423 - CA265_RS21115 0.38 -0.6 5,202,423 - CA265_RS21115 0.38 -0.9 5,202,519 - CA265_RS21115 0.82 +0.6 5,202,519 - CA265_RS21115 0.82 +0.9 5,202,519 - CA265_RS21115 0.82 -0.6 5,202,519 - CA265_RS21115 0.82 +0.7 5,202,581 - -1.4
Or see this region's nucleotide sequence