Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20995 and CA265_RS21000 are separated by 44 nucleotides CA265_RS21000 and CA265_RS21005 are separated by 27 nucleotides CA265_RS21005 and CA265_RS21010 are separated by 64 nucleotides CA265_RS21010 and CA265_RS21015 are separated by 0 nucleotides
CA265_RS20995: CA265_RS20995 - biotin--[acetyl-CoA-carboxylase] ligase, at 5,172,470 to 5,173,246
_RS20995
CA265_RS21000: CA265_RS21000 - ribosome silencing factor, at 5,173,291 to 5,173,665
_RS21000
CA265_RS21005: CA265_RS21005 - peptidase M41, at 5,173,693 to 5,175,708
_RS21005
CA265_RS21010: CA265_RS21010 - hypothetical protein, at 5,175,773 to 5,176,417
_RS21010
CA265_RS21015: CA265_RS21015 - hypothetical protein, at 5,176,418 to 5,177,524
_RS21015
Position (kb) 5173
5174
5175
5176 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5173.220 kb on + strand at 5173.262 kb on + strand at 5173.307 kb on + strand at 5173.320 kb on + strand at 5173.352 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.374 kb on + strand, within CA265_RS21000 at 5173.425 kb on + strand, within CA265_RS21000 at 5173.478 kb on + strand, within CA265_RS21000 at 5173.479 kb on - strand, within CA265_RS21000 at 5173.490 kb on + strand, within CA265_RS21000 at 5173.493 kb on - strand at 5173.494 kb on + strand, within CA265_RS21000 at 5173.552 kb on - strand, within CA265_RS21000 at 5173.616 kb on + strand, within CA265_RS21000 at 5173.668 kb on + strand at 5173.670 kb on + strand at 5173.670 kb on + strand at 5174.224 kb on - strand, within CA265_RS21005 at 5174.562 kb on - strand, within CA265_RS21005 at 5174.844 kb on - strand, within CA265_RS21005 at 5175.688 kb on - strand at 5175.688 kb on - strand at 5175.711 kb on - strand at 5175.758 kb on + strand at 5175.856 kb on + strand, within CA265_RS21010 at 5175.890 kb on - strand, within CA265_RS21010 at 5175.906 kb on + strand, within CA265_RS21010 at 5175.910 kb on - strand, within CA265_RS21010 at 5175.931 kb on + strand, within CA265_RS21010 at 5176.022 kb on + strand, within CA265_RS21010 at 5176.076 kb on + strand, within CA265_RS21010 at 5176.213 kb on + strand, within CA265_RS21010 at 5176.213 kb on + strand, within CA265_RS21010 at 5176.253 kb on - strand, within CA265_RS21010 at 5176.270 kb on - strand, within CA265_RS21010 at 5176.280 kb on - strand, within CA265_RS21010 at 5176.280 kb on - strand, within CA265_RS21010 at 5176.284 kb on - strand, within CA265_RS21010 at 5176.362 kb on + strand at 5176.379 kb on - strand at 5176.420 kb on - strand at 5176.428 kb on + strand at 5176.429 kb on - strand at 5176.439 kb on + strand at 5176.439 kb on + strand at 5176.440 kb on - strand at 5176.440 kb on - strand at 5176.484 kb on - strand at 5176.559 kb on + strand, within CA265_RS21015 at 5176.559 kb on + strand, within CA265_RS21015 at 5176.588 kb on + strand, within CA265_RS21015 at 5176.591 kb on + strand, within CA265_RS21015 at 5176.607 kb on + strand, within CA265_RS21015 at 5176.612 kb on + strand, within CA265_RS21015 at 5176.612 kb on + strand, within CA265_RS21015 at 5176.613 kb on - strand, within CA265_RS21015 at 5176.613 kb on - strand, within CA265_RS21015 at 5176.614 kb on + strand, within CA265_RS21015 at 5176.621 kb on + strand, within CA265_RS21015 at 5176.654 kb on - strand, within CA265_RS21015
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 5,173,220 + -0.3 5,173,262 + +0.8 5,173,307 + +0.2 5,173,320 + -1.0 5,173,352 + CA265_RS21000 0.16 +0.2 5,173,374 + CA265_RS21000 0.22 +0.2 5,173,374 + CA265_RS21000 0.22 -0.3 5,173,425 + CA265_RS21000 0.36 +0.3 5,173,478 + CA265_RS21000 0.50 -0.1 5,173,479 - CA265_RS21000 0.50 -1.3 5,173,490 + CA265_RS21000 0.53 +0.4 5,173,493 - +0.2 5,173,494 + CA265_RS21000 0.54 +0.2 5,173,552 - CA265_RS21000 0.70 +0.6 5,173,616 + CA265_RS21000 0.87 -0.4 5,173,668 + +1.2 5,173,670 + +0.5 5,173,670 + +0.2 5,174,224 - CA265_RS21005 0.26 -0.4 5,174,562 - CA265_RS21005 0.43 +2.2 5,174,844 - CA265_RS21005 0.57 -0.4 5,175,688 - -1.3 5,175,688 - -1.4 5,175,711 - -2.5 5,175,758 + -0.9 5,175,856 + CA265_RS21010 0.13 +0.5 5,175,890 - CA265_RS21010 0.18 +0.8 5,175,906 + CA265_RS21010 0.21 +0.2 5,175,910 - CA265_RS21010 0.21 -0.3 5,175,931 + CA265_RS21010 0.24 -0.7 5,176,022 + CA265_RS21010 0.39 +0.4 5,176,076 + CA265_RS21010 0.47 -1.2 5,176,213 + CA265_RS21010 0.68 +1.4 5,176,213 + CA265_RS21010 0.68 +0.1 5,176,253 - CA265_RS21010 0.74 -1.2 5,176,270 - CA265_RS21010 0.77 -0.7 5,176,280 - CA265_RS21010 0.79 -0.4 5,176,280 - CA265_RS21010 0.79 -1.0 5,176,284 - CA265_RS21010 0.79 -0.4 5,176,362 + +0.1 5,176,379 - -0.7 5,176,420 - +0.2 5,176,428 + -0.4 5,176,429 - -0.8 5,176,439 + +0.4 5,176,439 + +0.0 5,176,440 - +0.4 5,176,440 - +0.8 5,176,484 - +0.9 5,176,559 + CA265_RS21015 0.13 +0.9 5,176,559 + CA265_RS21015 0.13 -0.1 5,176,588 + CA265_RS21015 0.15 +2.8 5,176,591 + CA265_RS21015 0.16 -0.2 5,176,607 + CA265_RS21015 0.17 -0.3 5,176,612 + CA265_RS21015 0.18 -0.2 5,176,612 + CA265_RS21015 0.18 +0.2 5,176,613 - CA265_RS21015 0.18 -0.6 5,176,613 - CA265_RS21015 0.18 -0.3 5,176,614 + CA265_RS21015 0.18 -0.1 5,176,621 + CA265_RS21015 0.18 -0.4 5,176,654 - CA265_RS21015 0.21 +1.0
Or see this region's nucleotide sequence