Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19130 and CA265_RS19135 are separated by 357 nucleotides CA265_RS19135 and CA265_RS19140 are separated by 83 nucleotides CA265_RS19140 and CA265_RS19145 are separated by 255 nucleotides CA265_RS19145 and CA265_RS19150 are separated by 927 nucleotides
CA265_RS19130: CA265_RS19130 - 23S ribosomal RNA, at 4,752,409 to 4,755,288
_RS19130
CA265_RS19135: CA265_RS19135 - tRNA-Ala, at 4,755,646 to 4,755,722
_RS19135
CA265_RS19140: CA265_RS19140 - tRNA-Ile, at 4,755,806 to 4,755,880
_RS19140
CA265_RS19145: CA265_RS19145 - 16S ribosomal RNA, at 4,756,136 to 4,757,656
_RS19145
CA265_RS19150: CA265_RS19150 - amidophosphoribosyltransferase, at 4,758,584 to 4,759,282
_RS19150
Position (kb)
4756
4757
4758 Strain fitness (log2 ratio)
-1
0
1 at 4755.619 kb on - strand at 4755.740 kb on + strand at 4755.843 kb on - strand, within CA265_RS19140 at 4756.073 kb on - strand at 4756.078 kb on - strand at 4758.491 kb on + strand at 4758.491 kb on + strand at 4758.495 kb on + strand at 4758.495 kb on + strand at 4758.495 kb on + strand at 4758.495 kb on + strand at 4758.496 kb on - strand at 4758.497 kb on + strand at 4758.497 kb on + strand at 4758.500 kb on - strand at 4758.508 kb on + strand at 4758.605 kb on - strand at 4758.624 kb on + strand at 4758.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 4,755,619 - +0.6 4,755,740 + +1.6 4,755,843 - CA265_RS19140 0.49 +0.1 4,756,073 - -0.7 4,756,078 - +0.4 4,758,491 + +0.4 4,758,491 + +0.1 4,758,495 + +0.8 4,758,495 + +0.2 4,758,495 + +0.5 4,758,495 + +1.4 4,758,496 - -1.6 4,758,497 + -0.5 4,758,497 + +0.6 4,758,500 - -0.3 4,758,508 + +0.6 4,758,605 - +0.2 4,758,624 + -0.7 4,758,639 + +1.7
Or see this region's nucleotide sequence