Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12405 and CA265_RS12410 are separated by 146 nucleotides CA265_RS12410 and CA265_RS12415 are separated by 143 nucleotides CA265_RS12415 and CA265_RS12420 overlap by 35 nucleotides
CA265_RS12405: CA265_RS12405 - hypothetical protein, at 3,094,681 to 3,095,808
_RS12405
CA265_RS12410: CA265_RS12410 - hypothetical protein, at 3,095,955 to 3,096,950
_RS12410
CA265_RS12415: CA265_RS12415 - hypothetical protein, at 3,097,094 to 3,097,606
_RS12415
CA265_RS12420: CA265_RS12420 - hypothetical protein, at 3,097,572 to 3,099,518
_RS12420
Position (kb)
3095
3096
3097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3094.960 kb on - strand, within CA265_RS12405 at 3094.965 kb on - strand, within CA265_RS12405 at 3095.015 kb on + strand, within CA265_RS12405 at 3095.016 kb on - strand, within CA265_RS12405 at 3095.050 kb on - strand, within CA265_RS12405 at 3095.115 kb on - strand, within CA265_RS12405 at 3095.131 kb on - strand, within CA265_RS12405 at 3095.320 kb on - strand, within CA265_RS12405 at 3095.341 kb on + strand, within CA265_RS12405 at 3095.342 kb on - strand, within CA265_RS12405 at 3095.342 kb on - strand, within CA265_RS12405 at 3095.344 kb on - strand, within CA265_RS12405 at 3095.353 kb on - strand, within CA265_RS12405 at 3095.443 kb on - strand, within CA265_RS12405 at 3095.517 kb on + strand, within CA265_RS12405 at 3095.547 kb on - strand, within CA265_RS12405 at 3095.547 kb on - strand, within CA265_RS12405 at 3095.552 kb on - strand, within CA265_RS12405 at 3095.607 kb on + strand, within CA265_RS12405 at 3095.607 kb on + strand, within CA265_RS12405 at 3095.629 kb on + strand, within CA265_RS12405 at 3095.629 kb on + strand, within CA265_RS12405 at 3095.654 kb on + strand, within CA265_RS12405 at 3095.661 kb on - strand, within CA265_RS12405 at 3095.661 kb on - strand, within CA265_RS12405 at 3095.664 kb on + strand, within CA265_RS12405 at 3095.722 kb on + strand at 3095.722 kb on + strand at 3095.726 kb on - strand at 3095.847 kb on - strand at 3095.878 kb on + strand at 3095.879 kb on - strand at 3095.924 kb on - strand at 3095.998 kb on - strand at 3096.042 kb on + strand at 3096.046 kb on + strand at 3096.072 kb on + strand, within CA265_RS12410 at 3096.129 kb on + strand, within CA265_RS12410 at 3096.165 kb on - strand, within CA265_RS12410 at 3096.165 kb on - strand, within CA265_RS12410 at 3096.165 kb on - strand, within CA265_RS12410 at 3096.220 kb on + strand, within CA265_RS12410 at 3096.225 kb on - strand, within CA265_RS12410 at 3096.241 kb on + strand, within CA265_RS12410 at 3096.254 kb on + strand, within CA265_RS12410 at 3096.266 kb on - strand, within CA265_RS12410 at 3096.297 kb on + strand, within CA265_RS12410 at 3096.298 kb on - strand, within CA265_RS12410 at 3096.298 kb on - strand, within CA265_RS12410 at 3096.305 kb on + strand, within CA265_RS12410 at 3096.305 kb on + strand, within CA265_RS12410 at 3096.316 kb on - strand, within CA265_RS12410 at 3096.327 kb on + strand, within CA265_RS12410 at 3096.328 kb on - strand, within CA265_RS12410 at 3096.374 kb on + strand, within CA265_RS12410 at 3096.375 kb on - strand, within CA265_RS12410 at 3096.419 kb on + strand, within CA265_RS12410 at 3096.420 kb on - strand, within CA265_RS12410 at 3096.423 kb on - strand, within CA265_RS12410 at 3096.476 kb on + strand, within CA265_RS12410 at 3096.476 kb on + strand, within CA265_RS12410 at 3096.519 kb on - strand, within CA265_RS12410 at 3096.531 kb on + strand, within CA265_RS12410 at 3096.532 kb on - strand, within CA265_RS12410 at 3096.555 kb on + strand, within CA265_RS12410 at 3096.566 kb on + strand, within CA265_RS12410 at 3096.566 kb on + strand, within CA265_RS12410 at 3096.566 kb on + strand, within CA265_RS12410 at 3096.630 kb on - strand, within CA265_RS12410 at 3096.645 kb on - strand, within CA265_RS12410 at 3096.651 kb on + strand, within CA265_RS12410 at 3096.651 kb on + strand, within CA265_RS12410 at 3096.652 kb on - strand, within CA265_RS12410 at 3096.652 kb on - strand, within CA265_RS12410 at 3096.652 kb on - strand, within CA265_RS12410 at 3096.708 kb on - strand, within CA265_RS12410 at 3096.841 kb on - strand, within CA265_RS12410 at 3096.866 kb on + strand at 3096.892 kb on - strand at 3096.912 kb on + strand at 3096.913 kb on - strand at 3096.913 kb on - strand at 3096.924 kb on - strand at 3096.935 kb on + strand at 3096.936 kb on - strand at 3096.956 kb on + strand at 3097.090 kb on + strand at 3097.095 kb on + strand at 3097.133 kb on - strand at 3097.158 kb on + strand, within CA265_RS12415 at 3097.159 kb on - strand, within CA265_RS12415 at 3097.159 kb on - strand, within CA265_RS12415 at 3097.192 kb on + strand, within CA265_RS12415 at 3097.224 kb on - strand, within CA265_RS12415 at 3097.224 kb on - strand, within CA265_RS12415 at 3097.256 kb on + strand, within CA265_RS12415 at 3097.257 kb on - strand, within CA265_RS12415 at 3097.280 kb on + strand, within CA265_RS12415 at 3097.313 kb on - strand, within CA265_RS12415 at 3097.316 kb on + strand, within CA265_RS12415 at 3097.325 kb on - strand, within CA265_RS12415 at 3097.365 kb on - strand, within CA265_RS12415 at 3097.380 kb on - strand, within CA265_RS12415 at 3097.451 kb on - strand, within CA265_RS12415 at 3097.457 kb on + strand, within CA265_RS12415 at 3097.516 kb on + strand, within CA265_RS12415 at 3097.519 kb on - strand, within CA265_RS12415 at 3097.524 kb on - strand, within CA265_RS12415 at 3097.552 kb on + strand, within CA265_RS12415 at 3097.573 kb on + strand at 3097.607 kb on + strand at 3097.643 kb on + strand at 3097.651 kb on - strand at 3097.654 kb on + strand at 3097.698 kb on - strand at 3097.758 kb on - strand at 3097.787 kb on + strand, within CA265_RS12420 at 3097.787 kb on + strand, within CA265_RS12420 at 3097.788 kb on - strand, within CA265_RS12420 at 3097.788 kb on - strand, within CA265_RS12420 at 3097.813 kb on + strand, within CA265_RS12420 at 3097.813 kb on + strand, within CA265_RS12420 at 3097.814 kb on - strand, within CA265_RS12420 at 3097.814 kb on - strand, within CA265_RS12420 at 3097.870 kb on + strand, within CA265_RS12420 at 3097.921 kb on - strand, within CA265_RS12420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 3,094,960 - CA265_RS12405 0.25 +0.7 3,094,965 - CA265_RS12405 0.25 -0.6 3,095,015 + CA265_RS12405 0.30 +0.5 3,095,016 - CA265_RS12405 0.30 +0.4 3,095,050 - CA265_RS12405 0.33 +0.8 3,095,115 - CA265_RS12405 0.38 -0.1 3,095,131 - CA265_RS12405 0.40 +0.3 3,095,320 - CA265_RS12405 0.57 -0.3 3,095,341 + CA265_RS12405 0.59 -1.9 3,095,342 - CA265_RS12405 0.59 -0.2 3,095,342 - CA265_RS12405 0.59 -0.2 3,095,344 - CA265_RS12405 0.59 -0.2 3,095,353 - CA265_RS12405 0.60 +0.1 3,095,443 - CA265_RS12405 0.68 +0.3 3,095,517 + CA265_RS12405 0.74 -1.0 3,095,547 - CA265_RS12405 0.77 -0.8 3,095,547 - CA265_RS12405 0.77 +1.0 3,095,552 - CA265_RS12405 0.77 +0.9 3,095,607 + CA265_RS12405 0.82 -0.3 3,095,607 + CA265_RS12405 0.82 -0.1 3,095,629 + CA265_RS12405 0.84 -0.4 3,095,629 + CA265_RS12405 0.84 +0.1 3,095,654 + CA265_RS12405 0.86 +0.5 3,095,661 - CA265_RS12405 0.87 -0.1 3,095,661 - CA265_RS12405 0.87 +2.3 3,095,664 + CA265_RS12405 0.87 -0.7 3,095,722 + -0.5 3,095,722 + -0.6 3,095,726 - +0.1 3,095,847 - -0.4 3,095,878 + -0.1 3,095,879 - +0.1 3,095,924 - -1.4 3,095,998 - +0.2 3,096,042 + -1.3 3,096,046 + -1.1 3,096,072 + CA265_RS12410 0.12 -1.3 3,096,129 + CA265_RS12410 0.17 -0.3 3,096,165 - CA265_RS12410 0.21 +0.3 3,096,165 - CA265_RS12410 0.21 +0.3 3,096,165 - CA265_RS12410 0.21 +0.0 3,096,220 + CA265_RS12410 0.27 -0.8 3,096,225 - CA265_RS12410 0.27 -0.3 3,096,241 + CA265_RS12410 0.29 +0.3 3,096,254 + CA265_RS12410 0.30 -0.3 3,096,266 - CA265_RS12410 0.31 +0.5 3,096,297 + CA265_RS12410 0.34 +0.3 3,096,298 - CA265_RS12410 0.34 -0.2 3,096,298 - CA265_RS12410 0.34 +0.5 3,096,305 + CA265_RS12410 0.35 -0.1 3,096,305 + CA265_RS12410 0.35 +0.9 3,096,316 - CA265_RS12410 0.36 -2.7 3,096,327 + CA265_RS12410 0.37 +0.9 3,096,328 - CA265_RS12410 0.37 -0.5 3,096,374 + CA265_RS12410 0.42 +0.5 3,096,375 - CA265_RS12410 0.42 -0.3 3,096,419 + CA265_RS12410 0.47 -0.8 3,096,420 - CA265_RS12410 0.47 -0.4 3,096,423 - CA265_RS12410 0.47 +0.5 3,096,476 + CA265_RS12410 0.52 -1.6 3,096,476 + CA265_RS12410 0.52 -2.3 3,096,519 - CA265_RS12410 0.57 -1.2 3,096,531 + CA265_RS12410 0.58 -1.7 3,096,532 - CA265_RS12410 0.58 -0.0 3,096,555 + CA265_RS12410 0.60 -0.5 3,096,566 + CA265_RS12410 0.61 -0.5 3,096,566 + CA265_RS12410 0.61 -0.0 3,096,566 + CA265_RS12410 0.61 +0.5 3,096,630 - CA265_RS12410 0.68 -0.4 3,096,645 - CA265_RS12410 0.69 -0.3 3,096,651 + CA265_RS12410 0.70 -0.3 3,096,651 + CA265_RS12410 0.70 -0.3 3,096,652 - CA265_RS12410 0.70 +1.0 3,096,652 - CA265_RS12410 0.70 -0.4 3,096,652 - CA265_RS12410 0.70 -0.2 3,096,708 - CA265_RS12410 0.76 -0.0 3,096,841 - CA265_RS12410 0.89 -0.4 3,096,866 + -0.2 3,096,892 - +1.0 3,096,912 + -0.1 3,096,913 - -0.1 3,096,913 - -0.2 3,096,924 - +0.5 3,096,935 + -0.3 3,096,936 - +0.0 3,096,956 + -0.3 3,097,090 + -0.9 3,097,095 + -1.1 3,097,133 - -0.2 3,097,158 + CA265_RS12415 0.12 +0.1 3,097,159 - CA265_RS12415 0.13 -1.5 3,097,159 - CA265_RS12415 0.13 -0.7 3,097,192 + CA265_RS12415 0.19 -0.6 3,097,224 - CA265_RS12415 0.25 -0.5 3,097,224 - CA265_RS12415 0.25 -0.1 3,097,256 + CA265_RS12415 0.32 -0.1 3,097,257 - CA265_RS12415 0.32 -1.0 3,097,280 + CA265_RS12415 0.36 -0.0 3,097,313 - CA265_RS12415 0.43 +0.1 3,097,316 + CA265_RS12415 0.43 -0.7 3,097,325 - CA265_RS12415 0.45 +0.0 3,097,365 - CA265_RS12415 0.53 -0.4 3,097,380 - CA265_RS12415 0.56 +0.2 3,097,451 - CA265_RS12415 0.70 +0.0 3,097,457 + CA265_RS12415 0.71 +0.5 3,097,516 + CA265_RS12415 0.82 -0.1 3,097,519 - CA265_RS12415 0.83 -0.1 3,097,524 - CA265_RS12415 0.84 +0.3 3,097,552 + CA265_RS12415 0.89 +0.1 3,097,573 + -1.2 3,097,607 + +0.0 3,097,643 + +1.5 3,097,651 - -0.6 3,097,654 + -0.4 3,097,698 - +0.5 3,097,758 - -0.5 3,097,787 + CA265_RS12420 0.11 +1.0 3,097,787 + CA265_RS12420 0.11 +0.4 3,097,788 - CA265_RS12420 0.11 -0.0 3,097,788 - CA265_RS12420 0.11 +0.2 3,097,813 + CA265_RS12420 0.12 -0.7 3,097,813 + CA265_RS12420 0.12 -3.4 3,097,814 - CA265_RS12420 0.12 -0.0 3,097,814 - CA265_RS12420 0.12 -0.1 3,097,870 + CA265_RS12420 0.15 +1.0 3,097,921 - CA265_RS12420 0.18 +0.1
Or see this region's nucleotide sequence