Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07450 and CA265_RS07455 overlap by 4 nucleotides CA265_RS07455 and CA265_RS07460 are separated by 42 nucleotides CA265_RS07460 and CA265_RS07465 are separated by 78 nucleotides CA265_RS07465 and CA265_RS07470 are separated by 413 nucleotides CA265_RS07470 and CA265_RS07475 are separated by 165 nucleotides
CA265_RS07450: CA265_RS07450 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 1,882,742 to 1,883,530
_RS07450
CA265_RS07455: CA265_RS07455 - hypothetical protein, at 1,883,527 to 1,883,730
_RS07455
CA265_RS07460: CA265_RS07460 - deoxynucleoside kinase, at 1,883,773 to 1,884,387
_RS07460
CA265_RS07465: CA265_RS07465 - tryptophan--tRNA ligase, at 1,884,466 to 1,885,464
_RS07465
CA265_RS07470: CA265_RS07470 - hypothetical protein, at 1,885,878 to 1,886,078
_RS07470
CA265_RS07475: CA265_RS07475 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,886,244 to 1,886,969
_RS07475
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1883.530 kb on + strand at 1883.531 kb on - strand at 1883.586 kb on + strand, within CA265_RS07455 at 1883.588 kb on + strand, within CA265_RS07455 at 1883.589 kb on - strand, within CA265_RS07455 at 1883.735 kb on - strand at 1883.742 kb on + strand at 1883.742 kb on + strand at 1883.743 kb on - strand at 1883.747 kb on - strand at 1883.747 kb on - strand at 1883.775 kb on - strand at 1883.800 kb on + strand at 1883.802 kb on + strand at 1883.802 kb on + strand at 1883.848 kb on + strand, within CA265_RS07460 at 1883.853 kb on + strand, within CA265_RS07460 at 1883.983 kb on - strand, within CA265_RS07460 at 1883.994 kb on + strand, within CA265_RS07460 at 1883.995 kb on - strand, within CA265_RS07460 at 1883.998 kb on + strand, within CA265_RS07460 at 1884.049 kb on - strand, within CA265_RS07460 at 1884.052 kb on - strand, within CA265_RS07460 at 1884.052 kb on - strand, within CA265_RS07460 at 1884.075 kb on + strand, within CA265_RS07460 at 1884.098 kb on - strand, within CA265_RS07460 at 1884.106 kb on - strand, within CA265_RS07460 at 1884.130 kb on - strand, within CA265_RS07460 at 1884.169 kb on - strand, within CA265_RS07460 at 1884.363 kb on + strand at 1884.364 kb on - strand at 1884.461 kb on - strand at 1884.461 kb on - strand at 1885.635 kb on + strand at 1885.636 kb on - strand at 1885.636 kb on - strand at 1885.636 kb on - strand at 1885.772 kb on - strand at 1885.803 kb on + strand at 1885.812 kb on - strand at 1885.983 kb on - strand, within CA265_RS07470 at 1885.997 kb on - strand, within CA265_RS07470 at 1886.017 kb on + strand, within CA265_RS07470 at 1886.088 kb on - strand at 1886.324 kb on - strand, within CA265_RS07475 at 1886.326 kb on - strand, within CA265_RS07475 at 1886.329 kb on - strand, within CA265_RS07475 at 1886.463 kb on + strand, within CA265_RS07475 at 1886.464 kb on - strand, within CA265_RS07475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 1,883,530 + -3.5 1,883,531 - +0.2 1,883,586 + CA265_RS07455 0.29 -3.7 1,883,588 + CA265_RS07455 0.30 -0.8 1,883,589 - CA265_RS07455 0.30 +0.0 1,883,735 - -0.2 1,883,742 + -2.0 1,883,742 + -1.4 1,883,743 - +0.2 1,883,747 - -0.7 1,883,747 - -0.1 1,883,775 - -0.4 1,883,800 + +0.8 1,883,802 + -0.6 1,883,802 + -1.0 1,883,848 + CA265_RS07460 0.12 -0.8 1,883,853 + CA265_RS07460 0.13 -1.1 1,883,983 - CA265_RS07460 0.34 +1.3 1,883,994 + CA265_RS07460 0.36 -0.6 1,883,995 - CA265_RS07460 0.36 +1.1 1,883,998 + CA265_RS07460 0.37 -1.6 1,884,049 - CA265_RS07460 0.45 +1.2 1,884,052 - CA265_RS07460 0.45 +1.7 1,884,052 - CA265_RS07460 0.45 +1.1 1,884,075 + CA265_RS07460 0.49 -2.0 1,884,098 - CA265_RS07460 0.53 +1.5 1,884,106 - CA265_RS07460 0.54 +1.6 1,884,130 - CA265_RS07460 0.58 +1.5 1,884,169 - CA265_RS07460 0.64 +1.2 1,884,363 + -2.3 1,884,364 - +1.2 1,884,461 - -0.2 1,884,461 - +0.0 1,885,635 + -1.4 1,885,636 - -0.4 1,885,636 - +0.5 1,885,636 - -0.2 1,885,772 - -0.5 1,885,803 + +0.9 1,885,812 - +0.8 1,885,983 - CA265_RS07470 0.52 -0.0 1,885,997 - CA265_RS07470 0.59 +0.2 1,886,017 + CA265_RS07470 0.69 -0.0 1,886,088 - +0.5 1,886,324 - CA265_RS07475 0.11 -1.0 1,886,326 - CA265_RS07475 0.11 -1.6 1,886,329 - CA265_RS07475 0.12 -0.5 1,886,463 + CA265_RS07475 0.30 -0.1 1,886,464 - CA265_RS07475 0.30 +0.1
Or see this region's nucleotide sequence