Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04260 and CA265_RS04265 are separated by 52 nucleotides CA265_RS04265 and CA265_RS04270 are separated by 157 nucleotides CA265_RS04270 and CA265_RS04275 overlap by 17 nucleotides
CA265_RS04260: CA265_RS04260 - trigger factor, at 1,031,256 to 1,032,608
_RS04260
CA265_RS04265: CA265_RS04265 - hypothetical protein, at 1,032,661 to 1,033,017
_RS04265
CA265_RS04270: CA265_RS04270 - ATP-dependent Clp protease proteolytic subunit, at 1,033,175 to 1,033,882
_RS04270
CA265_RS04275: CA265_RS04275 - ATP-dependent protease ATP-binding subunit ClpX, at 1,033,866 to 1,035,104
_RS04275
Position (kb)
1033
1034 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1032.282 kb on + strand, within CA265_RS04260 at 1032.283 kb on - strand, within CA265_RS04260 at 1032.370 kb on + strand, within CA265_RS04260 at 1032.371 kb on - strand, within CA265_RS04260 at 1032.415 kb on - strand, within CA265_RS04260 at 1032.415 kb on - strand, within CA265_RS04260 at 1032.453 kb on + strand, within CA265_RS04260 at 1032.454 kb on - strand, within CA265_RS04260 at 1032.508 kb on + strand at 1032.509 kb on - strand at 1032.509 kb on - strand at 1032.553 kb on + strand at 1032.574 kb on - strand at 1032.630 kb on - strand at 1032.641 kb on - strand at 1032.642 kb on + strand at 1032.642 kb on + strand at 1032.690 kb on - strand at 1032.690 kb on - strand at 1032.705 kb on + strand, within CA265_RS04265 at 1032.707 kb on + strand, within CA265_RS04265 at 1032.707 kb on + strand, within CA265_RS04265 at 1032.720 kb on + strand, within CA265_RS04265 at 1032.725 kb on + strand, within CA265_RS04265 at 1032.803 kb on + strand, within CA265_RS04265 at 1032.803 kb on + strand, within CA265_RS04265 at 1032.804 kb on - strand, within CA265_RS04265 at 1032.808 kb on - strand, within CA265_RS04265 at 1032.834 kb on + strand, within CA265_RS04265 at 1032.840 kb on + strand, within CA265_RS04265 at 1032.957 kb on - strand, within CA265_RS04265 at 1032.978 kb on - strand, within CA265_RS04265 at 1033.053 kb on - strand at 1033.078 kb on - strand at 1033.111 kb on + strand at 1034.040 kb on - strand, within CA265_RS04275 at 1034.041 kb on + strand, within CA265_RS04275 at 1034.085 kb on + strand, within CA265_RS04275 at 1034.086 kb on - strand, within CA265_RS04275 at 1034.120 kb on - strand, within CA265_RS04275 at 1034.120 kb on - strand, within CA265_RS04275 at 1034.169 kb on - strand, within CA265_RS04275 at 1034.341 kb on + strand, within CA265_RS04275 at 1034.398 kb on + strand, within CA265_RS04275 at 1034.399 kb on - strand, within CA265_RS04275 at 1034.399 kb on - strand, within CA265_RS04275 at 1034.403 kb on - strand, within CA265_RS04275 at 1034.418 kb on - strand, within CA265_RS04275 at 1034.448 kb on - strand, within CA265_RS04275 at 1034.507 kb on + strand, within CA265_RS04275 at 1034.515 kb on + strand, within CA265_RS04275 at 1034.516 kb on - strand, within CA265_RS04275 at 1034.582 kb on + strand, within CA265_RS04275 at 1034.583 kb on - strand, within CA265_RS04275 at 1034.583 kb on - strand, within CA265_RS04275 at 1034.587 kb on + strand, within CA265_RS04275 at 1034.588 kb on - strand, within CA265_RS04275 at 1034.588 kb on - strand, within CA265_RS04275 at 1034.592 kb on - strand, within CA265_RS04275 at 1034.645 kb on + strand, within CA265_RS04275 at 1034.713 kb on - strand, within CA265_RS04275 at 1034.714 kb on + strand, within CA265_RS04275 at 1034.715 kb on - strand, within CA265_RS04275 at 1034.715 kb on - strand, within CA265_RS04275 at 1034.802 kb on - strand, within CA265_RS04275 at 1034.856 kb on + strand, within CA265_RS04275 at 1034.857 kb on - strand, within CA265_RS04275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 1,032,282 + CA265_RS04260 0.76 -0.5 1,032,283 - CA265_RS04260 0.76 -1.0 1,032,370 + CA265_RS04260 0.82 -1.0 1,032,371 - CA265_RS04260 0.82 -1.5 1,032,415 - CA265_RS04260 0.86 -0.7 1,032,415 - CA265_RS04260 0.86 -1.0 1,032,453 + CA265_RS04260 0.88 -0.8 1,032,454 - CA265_RS04260 0.89 -1.3 1,032,508 + -0.4 1,032,509 - -1.4 1,032,509 - -0.5 1,032,553 + -0.6 1,032,574 - -1.8 1,032,630 - -0.9 1,032,641 - -0.2 1,032,642 + -0.3 1,032,642 + +0.5 1,032,690 - -1.0 1,032,690 - +0.6 1,032,705 + CA265_RS04265 0.12 +0.2 1,032,707 + CA265_RS04265 0.13 +1.2 1,032,707 + CA265_RS04265 0.13 -1.6 1,032,720 + CA265_RS04265 0.17 -0.4 1,032,725 + CA265_RS04265 0.18 +0.7 1,032,803 + CA265_RS04265 0.40 +0.7 1,032,803 + CA265_RS04265 0.40 -0.2 1,032,804 - CA265_RS04265 0.40 -1.4 1,032,808 - CA265_RS04265 0.41 -0.9 1,032,834 + CA265_RS04265 0.48 +1.0 1,032,840 + CA265_RS04265 0.50 +1.4 1,032,957 - CA265_RS04265 0.83 -2.0 1,032,978 - CA265_RS04265 0.89 -1.4 1,033,053 - -2.0 1,033,078 - +1.0 1,033,111 + +1.0 1,034,040 - CA265_RS04275 0.14 -2.4 1,034,041 + CA265_RS04275 0.14 +0.8 1,034,085 + CA265_RS04275 0.18 +2.0 1,034,086 - CA265_RS04275 0.18 -1.0 1,034,120 - CA265_RS04275 0.21 -1.4 1,034,120 - CA265_RS04275 0.21 -2.4 1,034,169 - CA265_RS04275 0.24 -4.3 1,034,341 + CA265_RS04275 0.38 +1.1 1,034,398 + CA265_RS04275 0.43 +0.4 1,034,399 - CA265_RS04275 0.43 -2.0 1,034,399 - CA265_RS04275 0.43 -2.3 1,034,403 - CA265_RS04275 0.43 -2.4 1,034,418 - CA265_RS04275 0.45 -2.4 1,034,448 - CA265_RS04275 0.47 -2.8 1,034,507 + CA265_RS04275 0.52 +1.7 1,034,515 + CA265_RS04275 0.52 +0.8 1,034,516 - CA265_RS04275 0.52 -2.5 1,034,582 + CA265_RS04275 0.58 +0.7 1,034,583 - CA265_RS04275 0.58 -2.4 1,034,583 - CA265_RS04275 0.58 -4.0 1,034,587 + CA265_RS04275 0.58 +0.3 1,034,588 - CA265_RS04275 0.58 -3.8 1,034,588 - CA265_RS04275 0.58 -4.6 1,034,592 - CA265_RS04275 0.59 +1.2 1,034,645 + CA265_RS04275 0.63 +1.4 1,034,713 - CA265_RS04275 0.68 -0.8 1,034,714 + CA265_RS04275 0.68 +1.5 1,034,715 - CA265_RS04275 0.69 -2.4 1,034,715 - CA265_RS04275 0.69 -3.9 1,034,802 - CA265_RS04275 0.76 -1.6 1,034,856 + CA265_RS04275 0.80 +0.7 1,034,857 - CA265_RS04275 0.80 -2.9
Or see this region's nucleotide sequence