Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2364 and Shew_2365 are separated by 106 nucleotides Shew_2365 and Shew_2366 are separated by 99 nucleotides Shew_2366 and Shew_2367 are separated by 102 nucleotides Shew_2367 and Shew_2368 are separated by 138 nucleotides
Shew_2364: Shew_2364 - aldehyde dehydrogenase (RefSeq), at 2,764,957 to 2,766,522
_2364
Shew_2365: Shew_2365 - GntR family transcriptional regulator (RefSeq), at 2,766,629 to 2,767,300
_2365
Shew_2366: Shew_2366 - 3-oxoacyl-(acyl carrier protein) synthase III (RefSeq), at 2,767,400 to 2,768,467
_2366
Shew_2367: Shew_2367 - hypothetical protein (RefSeq), at 2,768,570 to 2,769,160
_2367
Shew_2368: Shew_2368 - exonuclease, RNase T and DNA polymerase III (RefSeq), at 2,769,299 to 2,769,979
_2368
Position (kb)
2767
2768
2769 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2766.504 kb on - strand at 2766.783 kb on + strand, within Shew_2365 at 2767.485 kb on + strand at 2767.776 kb on - strand, within Shew_2366 at 2768.041 kb on - strand, within Shew_2366 at 2768.565 kb on - strand at 2768.668 kb on + strand, within Shew_2367 at 2768.880 kb on - strand, within Shew_2367 at 2768.994 kb on + strand, within Shew_2367 at 2769.181 kb on + strand at 2769.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 2,766,504 - -0.6 2,766,783 + Shew_2365 0.23 -4.8 2,767,485 + +1.6 2,767,776 - Shew_2366 0.35 -0.3 2,768,041 - Shew_2366 0.60 +4.2 2,768,565 - +0.5 2,768,668 + Shew_2367 0.17 -0.1 2,768,880 - Shew_2367 0.52 +0.9 2,768,994 + Shew_2367 0.72 +0.1 2,769,181 + -0.4 2,769,189 - +0.1
Or see this region's nucleotide sequence