Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgE and flgF are separated by 199 nucleotides flgF and flgG are separated by 10 nucleotides flgG and flgH are separated by 9 nucleotides flgH and flgI are separated by 11 nucleotides
Shew_1348: flgE - flagellar hook protein FlgE (RefSeq), at 1,556,368 to 1,557,729
flgE
Shew_1349: flgF - flagellar basal body rod protein FlgF (RefSeq), at 1,557,929 to 1,558,672
flgF
Shew_1350: flgG - flagellar basal body rod protein FlgG (RefSeq), at 1,558,683 to 1,559,471
flgG
Shew_1351: flgH - flagellar basal body L-ring protein (RefSeq), at 1,559,481 to 1,560,185
flgH
Shew_1352: flgI - flagellar basal body P-ring protein (RefSeq), at 1,560,197 to 1,561,288
flgI
Position (kb) 1558
1559
1560 Strain fitness (log2 ratio) -1
0
1
2 at 1557.710 kb on + strand at 1558.048 kb on + strand, within flgF at 1558.061 kb on - strand, within flgF at 1558.227 kb on - strand, within flgF at 1558.313 kb on + strand, within flgF at 1558.774 kb on + strand, within flgG at 1559.289 kb on + strand, within flgG at 1559.413 kb on + strand at 1560.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 1,557,710 + -0.3 1,558,048 + flgF Shew_1349 0.16 +2.2 1,558,061 - flgF Shew_1349 0.18 +0.2 1,558,227 - flgF Shew_1349 0.40 +0.7 1,558,313 + flgF Shew_1349 0.52 +0.2 1,558,774 + flgG Shew_1350 0.12 -0.2 1,559,289 + flgG Shew_1350 0.77 -0.4 1,559,413 + -0.6 1,560,280 + -0.1
Or see this region's nucleotide sequence